The collection of Bathynellacea specimens of MNCN (CSIC) Madrid: microscope slices and DNA extract

Abstract

This is the first published database of a Bathynellacea Chappuis, 1915 collection of slices and DNA extracts. It includes all data of bathynellaceans (Crustacea: Syncarida) collected in the last 48 years (1968 to 2016) on the Iberian Peninsula and Balearic Islands, studied since 1984. It also includes specimens studied across many countries of Europe (Portugal, Romania, France, Italy, Slovenia, Bulgaria, and England), as well as some specimens obtained from samples of North America (Montana, Washington, Alaska and Texas), South America (Brazil, Chile and Argentina), Asia (China, Thailand, Vietnam, Mongolia and India), Africa (Morocco and Chad) and Australia (New South Wales –NSW- and Queensland). The samples come from groundwater (caves, springs, wells and hyporrheic habitat associated with rivers) obtained from both, sampling campaigns and occasional sampling efforts.The data set includes 3399 records (2657 slices and 742 DNA extracts) corresponding to three families (Parabathynellidae Noodt, 1965, Leptobathynellidae Noodt, 1965 and Bathynellidae Grobben, 1905) of the order Bathynellacea; the existence of three families is accepted, but this is a controversial issue and here is not the appropriate context to address this problem; 52 genera and 92 species formally described, in addition to 30 taxa under study and, thus, still unpublished. This represents more than half of all the genera known worldwide (80) and almost one third of the species currently known in the world (329, which increases every year).This dataset contains especially relevant collection that includes holotypes and type series of 43 new species of Bathynellacea (33 from the Parabathynellidae and ten from the Bathynellidae) described by Ana I. Camacho (AIC hereinafter); eleven of these are the type species for new genera described from all around the world, ten belonging to the Parabathynellidae and one from the Bathynellidae. As previously mentioned, these new species come from all continents, although 26 of them are from the Iberian Peninsula.The most important feature of this collection is that it has been created and reviewed by a specialist of the group (AIC), and each specimen, regardless of its shape (either permanent slices or DNA extracts), includes taxonomic, geographical and authorship information. The specialist has been involved in all stages of the process, from field sampling to the digitization of the results we are now presenting, and has worked in close collaboration with the curators responsible for the different collections involved in this project.

Molecular phylogeny of Parabathynellidae (Crustacea, Bathynellacea), and three new species from Thai caves

“…We included 25 specimens of 6 genera of non-Asiatic Parabathynellidae as outgroups (Iberobathynella Schminke, 1973, Paraiberobathynella Camacho and Serban, 1998, Parabathynella Chappuis, 1926, Hexabathynella Schminke, 1972, Habrobathynella and Paraeobathynella) sequenced previously by us, plus GenBank sequences of nine specimens of six Australian genera (Brevisomabathynella Cho, Park and Ranga Reddy, 2006, Billibathynella Cho, 2005, Atopobathynella Schminke, 1973, Octobathynella Camacho and Hancock, 2010, Lockyerenella Camacho and Little, 2016 and Arkaroolabathynella Abrams and King, 2013) and one of the cosmopolitan genus Hexabathynella (also from Australia; Table 2). The species Vejdovskybathynella edelweiss Camacho, 2007, from Spain -a representative of the Parabathynellidae's sister lineage Bathynellidae Grobben, 1905 (Camacho et al, 2017)-, and Anaspides tasmaniae Thomson, 1893, from Tasmania, were chosen as more distant outgroups.…”

“…1) parabathynellids were found in three different locations. These taxa represent three new species of the family Parabathynellidae Noodt, 1965, which is widespread around the world, with 45 genera and 209 species described thus far (Camacho et al, 2017). The family has been poorly studied in Asia, where 17 genera and 74 species occur (see Appendix 2) (Bandari et al, 2016(Bandari et al, , 2017Camacho, 2005a;Camacho et al, 2006Camacho et al, , 2011Cho et al, 2008Cho et al, , 2015Nam and Cho, 2014;Park and Cho, 2008Ranga Reddy, 2002, 2004Schminke, 2005, 2009;Ranga Reddy et al, 2008Totakura, 2010, 2015;Serban, 1994;Totakura and Reddy, 2014).…”

Revision of the subterranean genus Spelaeodiscus Brusina, 1886 (Gastropoda, Pulmonata, Spelaeodiscidae)

“…Boettger, 1935 and Tsoukatosia Gittenberger, 2000 have affinity to the superficial underground compartments (also known as “Mesovoid Shallow Substratum” or “Milieu Souterrain Superficiel”, widely abbreviated as MSS, see e.g. Juberthie et al 1980, 1981; Camacho 1992; Culver and Pipan 2009, Mammola et al 2016). …”

Range‐constrained co‐occurrence simulation reveals little niche partitioning among rock‐dwelling Montenegrina land snails (Gastropoda: Clausiliidae)

“…Several of these, including other genera in the subfamily Alopiinae, are obligate rock-dwellers like Montenegrina . Others can be found in the same rocky habitats as Montenegrina , but without being obligate rock-dwellers, whereas others inhabit the superficial underground compartments around the rocks (Camacho, 1992: 65).…”