The collagen and hexosamine content of the skin of normal and experimentally treated rats

Sobel, Harry; Zutrauen, Hermann A.; Marmorston, Jessie

doi:10.1016/0003-9861(53)90184-2

Cited by 48 publications

(12 citation statements)

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“…Changes in the cellular microenvironment contribute to or may play a direct role in the genesis of cellular age changes by reducing rate of transfer of molecules to and from the cells. These conclusions were derived from studies on the changes which occurr ed in the skin and femurs of growing rats [76,77]. Collagen accrued at a faster rate than the hexosamine-containing constituents of the ground substance of connective tissue.…”

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confidence: 99%

Aging of Connective Tissue and Molecular Transport

Sobel¹

1968

Gerontology

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confidence: 99%

Aging of Connective Tissue and Molecular Transport

Sobel¹

1968

Gerontology

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“…Several observations seem to prove that sodium salicylate decreases the uptake of sulfate-S85 by slices of cartilage in vitro (21) and the hexosamine content of the skin in vivo (22), effects which have 9 Brand name of 17-hydroxy-11-dehydrocorticosterone. l519 NICOLA DI FERRANTE been interpreted as indicative of inhibition in the synthesis of acid mucopolysaccharides produced by this drug.…”

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confidence: 99%

Urinary Excretion of Acid Mucopolysaccharides by Patients with Rheumatoid Arthritis1

Ferrante¹

1957

J. Clin. Invest.

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Several methods have been described for the determination of urinary acid mucopolysaccharides (1-4). It has been found that in normal individuals the daily urinary excretion varies with age and sex (4,5). A few attempts have been made to correlate urinary excretion of acid mucopolysaccharides with different disease states. Craddock and Kerby (6) have reported that patients with impairment of renal function have a urinary excretion of acid mucopolysaccharides lower than that of normal individuals. They also found no significant differences between normal individuals and diabetic patients without renal involvement. In patients with rheumatoid arthritis Badin, Schubert, and Vouras (7) have demonstrated a high plasma concentration of acid mucopolysaccharides, but there have hitherto been no data available concerning their urinary excretion. This paper presents data on the urinary excretion of acid mucopolysaccharides by patients with rheumatoid arthritis and discusses the identity of the urinary acid mucopolysaccharides excreted in this disease. MATERIAL AND METHODThe study was performed on eight patients hospitalized for active rheumatoid arthritis. All of them showed a moderately elevated erythrocyte sedimentation rate, low-grade fever, involvement of several joints, and typical radiographic alterations. All patients were untreated when the urine collection was started. Two of them, however (M.G. and P.C.), discontinued treatment a few days before the urine collection was started. Urinary acid mucopolysaccharides were determined according to the method described by Di Ferrante and Rich (4), by which the urinary acid mucopolysaccharides are precipitated, from an aliquot of the 24-hour urine, with cetyl-trimethyl-ammonium bromide. After removal of the precipitant the glucuronic acid content of the precipitate is determined. The results in Table I In five patients (P.C., B.E., E.E., W.J., and G.A.) the urinary excretion of acid mucopolysaccharides was measured both before and during periods of oral treatment with sodium salicylate.In order to collect an amount of urinary acid mucopolysaccharides sufficient for purification and analysis, 20 liters of pooled urine from patients with rheumatoid arthritis were adjusted to pH 5 with 2 N HCl, and 8 Gm. of cetyl-trimethyl-ammonium bromide were added. After 24 hours at 00 C., the fine precipitate present was collected, by filtration under suction, on a thin layer of Hyflo super-cel. The cake of Hyflo super-cel was then washed in a 250-ml. centrifuge bottle with several 150-ml. aliquots of 95 per cent ethyl alcohol saturated in NaCl, until the supernatant obtained after centrifugation was colorless. The sediment was dried in a desiccator, under vacuum and in presence of paraffin chips, and then extracted three times with 30 ml. of distilled water. The aqueous extract was reprecipitated with three volumes of 95 per cent ethyl alcohol saturated in NaCl.After 24 hours at 00 C., the precipitate was collected by centrifugation, dissolved in 100 ml. of water and cleared of insolub...

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“…It is to be stressed that in the great majority of works where the ratio AMPS/collagen has been calculated, a decrease with advancing age was reported to occur in the organs of the species examined [1,2,4,7,11]. This indicates a steady increase of the fibrous elements in proportion to the ground substance.…”

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confidence: 99%

“…The concentration of AMPS in various organs has been found to decrease [1][2][3][4], to increase [5][6][7], or to remain constant [8]. Total col lagen was generally found to increase with age in a number of organs examined [1,4,6,9,10,11]. McGavack and Kao [12], in their studies on the quantitative variations in total collagen content of rat tissues in relation to age, reported an increase in a number of tissues while in others -kidneys included -no increase was observed.…”

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confidence: 99%