The cold shock response inLactococcus lactis subsp.lactis

Panoff, Jean-Michel; Legrand, Stéphane; Thammavongs, Bouachanh; Boutibonnes, P.

doi:10.1007/bf01570156

Cited by 52 publications

(33 citation statements)

References 34 publications

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“…Indeed, the variations were more important (C16.0 and D24.5) and/or happened earlier (C7.0 and C8.0) and/or lasted longer (C7.0, C8.0, C17.0, D24.5, D30.2, E7.0, E8.0, and E31.0) in the 30-to-5°C cold shock. This relationship between the downshift amplitude and protein variations has been observed for other bacteria (1,3,18,31,37) and the psychrotrophic yeast T. pullullans (22). This finding indicates that to cope with cold shock, microorganisms regulate protein expression and particularly a subset of polypeptides which probably play an essential role in the adaptation to low temperature.…”

Section: Discussionsupporting

confidence: 69%

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The cold shock response of the psychrotrophic bacterium Pseudomonas fragi involves four low-molecular-mass nucleic acid-binding proteins

Michel¹,

Lehoux²,

Depret³

et al. 1997

J Bacteriol

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The psychrotrophic bacterium Pseudomonas fragi was subjected to cold shocks from 30 or 20 to 5°C. The downshifts were followed by a lag phase before growth resumed at a characteristic 5°C growth rate. The analysis of protein patterns by two-dimentional gel electrophoresis revealed overexpression of 25 or 17 proteins and underexpression of 12 proteins following the 30-or 20-to-5°C shift, respectively. The two downshifts shared similar variations of synthesis of 20 proteins. The kinetic analysis distinguished the induced proteins into cold shock proteins (Csps), which were rapidly but transiently overexpressed, and cold acclimation proteins (Caps), which were more or less rapidly induced but still overexpressed several hours after the downshifts. Among the cold-induced proteins, four low-molecular-mass proteins, two of them previously characterized as Caps (CapA and CapB), and heat acclimation proteins (Haps) as well as heat shock proteins (Hsps) for the two others (TapA and TapB) displayed higher levels of induction. Partial amino acid sequences, obtained by microsequencing, were used to design primers to amplify by PCR the four genes and then determine their nucleotide sequences. A BamHI-EcoRI restriction fragment of 1.9 kb, containing the complete coding sequence for capB, was cloned and sequenced. The four peptides belong to the family of small nucleic acid-binding proteins as CspA, the major Escherichia coli Csp. They are likely to play a major role in the adaptative response of P. fragi to environmental temperature changes.

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Section: Discussionsupporting

confidence: 69%

“…(1) and the mesophile Lactococcus lactis subsp. lactis (37) or B. subtilis (29) had no lag phase consequent to a cold treatment. Their growth continued at an intermediate rate followed by a growth rate characteristic of final low temperature.…”

Section: Discussionmentioning

confidence: 95%

The cold shock response of the psychrotrophic bacterium Pseudomonas fragi involves four low-molecular-mass nucleic acid-binding proteins

Michel¹,

Lehoux²,

Depret³

et al. 1997

J Bacteriol

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“…Although several genes involved in the heat shock response, such as dnaK, dnaJ, and groESL, have been shown to be conserved in L. lactis (3,16,37), no gene involved in the cold shock response has yet been identified. However, previous studies indicated that L. lactis responds to cold shock by induction of synthesis of a dozen proteins (25) and cold adaptation can be observed (26). Recently, a gene homologous to the E. coli cspA gene was partially cloned and sequenced after PCR amplification (6).…”

mentioning

confidence: 99%

Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene

et al. 1997

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Upon temperature downshift, the major cold shock protein CspA is highly induced in Escherichia coli. This protein being conserved in other bacteria, we used a PCR-based approach with a pair of degenerate primers derived from highly conserved regions of the CspA-related proteins to evidence the presence of at least three related genes in Lactococcus lactis. One of them, cspB, was cloned and sequenced. It encodes a 66-residue protein which possesses 60% sequence identity with E. coli CspA. Following a cold shock from 30 to 15°C, the level of the cspB mRNA transcript increased, as shown by Northern blot hybridization. In addition, induction of cspB-directed ␤-galactosidase activity was observed. These results indicate that the L. lactis cspB gene is cold shock inducible.

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“…Furthermore, a long-term survival of Lactococcus lactis ssp. lactis after freeze-drying was observed in the presence of sucrose (Chavarri et al, 1988).It was suggested that, by raising the glass-phase transition temperature, sugars can protect lactic acid bacteria cells to reaching the glassy phase without nucleating intracellular ice, it implied that, sugars, especially trehalose and sucrose, play a critical role in maintaining the cytoplasmic membrane properties stably during freeze drying (Panoff et al, 1994, Carvalho et al, 2003, Passot et al, 2015. Additionally, during dry-process, sugars were also suggested to perform desiccation tolerance by forming hydrogen bonds to proteins so that the tertiary protein structure was maintained in the absence of water (Leslie et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

Zhang

Hui

Chen

et al. 2017

Emir. J. Food Agric

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The cold shock response inLactococcus lactis subsp.lactis

Cited by 52 publications

References 34 publications

The cold shock response of the psychrotrophic bacterium Pseudomonas fragi involves four low-molecular-mass nucleic acid-binding proteins

The cold shock response of the psychrotrophic bacterium Pseudomonas fragi involves four low-molecular-mass nucleic acid-binding proteins

Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

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