Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene

Chapot‐Chartier, Marie‐Pierre; Schouler, Catherine; Lepeuple, A; Gripon, J.C.; Chopin, Marie-Christine

doi:10.1128/jb.179.17.5589-5593.1997

Cited by 29 publications

(12 citation statements)

References 38 publications

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“…One group consists of CspA and CspC, which have 80% identical residues, and these CSPs have a pI of 9; the other group includes CspB, CspD, and CspE, which have up to 85% identical residues, and these CSPs have a pI of 5 (35). Upon cold shock of L. lactis IL1403 by transfer from 30 to 15°C, 10-fold induction of cspB-directed ␤-galactosidase activity is observed (4). Similar cold-induced expression has been reported for E. coli cspA, and the data revealed that the transient induction of E. coli CspA occurs at the level of transcription (14) and at the level of mRNA stabilization (1,8,9).…”

supporting

confidence: 64%

Physiological and Regulatory Effects of Controlled Overproduction of Five Cold Shock Proteins of Lactococcus lactis MG1363

Wouters

Mailhes

Rombouts

et al. 2000

Appl Environ Microbiol

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The physiological and regulatory effects of overproduction of five cold shock proteins (CSPs) of Lactococcus lactis were studied. CspB, CspD, and CspE could be overproduced at high levels (up to 19% of the total protein), whereas for CspA and CspC limited overproduction (0.3 to 0.5% of the total protein) was obtained. Northern blot analysis revealed low abundance of the cspC transcript, indicating that the stability of cspC mRNA is low. The limited overproduction of CspA is likely to be caused by low stability of CspA since when there was an Arg-Pro mutation at position 58, the level of CspA production increased. Using two-dimensional gel electrophoresis, it was found that upon overproduction of the CSPs several proteins, including a number of coldinduced proteins of L. lactis, were induced. Strikingly, upon overproduction of CspC induction of CspB, putative CspF, and putative CspG was also observed. Overproduction of CspB and overproduction of CspE result in increased survival when L. lactis is frozen (maximum increases, 10-and 5-fold, respectively, after 4 freeze-thaw cycles). It is concluded that in L. lactis CSPs play a regulatory role in the cascade of events that are initiated by cold shock treatment and that they either have a direct protective effect during freezing (e.g., RNA stabilization) or induce other factors involved in the freeze-adaptive response or both.In a variety of bacteria, cold shock proteins (CSPs) are the major induced proteins upon exposure to cold shock. Different functions, e.g., as transcriptional activators, RNA chaperones, and anti-freeze proteins, have been attributed to CSPs (for reviews see references 11 and 36). CspA of Escherichia coli and CspB of Bacillus subtilis have been shown to bind singlestranded DNA, and E. coli CspA has been shown to act as a transcriptional activator for the hns and gyrA genes encoding proteins involved in DNA supercoiling (2,17,22). E. coli CspA and B. subtilis CspB B have very similar five-stranded ␤-barrel structures with several outward-facing residues important for single-stranded DNA binding. Furthermore, CSPs contain two highly conserved RNA-binding motifs, named RNP-1 and RNP-2, and indeed, mRNA-binding capacity has been demonstrated for E. coli CspA and B. subtilis CspB (13,15). It has been proposed that members of the CSP family bind to RNA in a cooperative manner and function as RNA chaperones, thereby facilitating the translation process (13). Disruption of B. subtilis cspB results in a freeze-sensitive phenotype (33) and also affects the level of induction of other cold-induced proteins (CIPs) upon temperature downshock in B. subtilis (12). Deletion of three CSPs in B. subtilis was shown to be lethal (13). Since not all members of the CSP family are cold induced, it has been suggested that CSPs play a role in multiple cellular processes, such as chromosomal condensation and/or cell division (36).The mesophilic lactic acid bacterium Lactococcus lactis is widely used to start industrial food fermentations. A variety of genes involved in t...

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supporting

confidence: 64%

Physiological and Regulatory Effects of Controlled Overproduction of Five Cold Shock Proteins of Lactococcus lactis MG1363

Wouters

Mailhes

Rombouts

et al. 2000

Appl Environ Microbiol

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“…In a third attempt, a central region of 75 bp of an L. casei csp gene was amplified with a set of degenerate primers (Table 1) previously used to amplify cspA ‐like gene fragments of Lactococcus lactis MG1363 (Chapot‐Chartier et al ., 1997) (Fig. 2a).…”

Section: Resultsmentioning

confidence: 99%

Cloning and characterization of a gene encoding a cold-shock protein inLactobacillus casei

Sauvageot

Beaufils

Mazé

et al. 2006

FEMS Microbiology Letters

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One csp-like gene, called cspA, from the lactic acid bacterium Lactobacillus casei was identified by an inverse polymerase chain reaction approach based on degenerate primers. cspA encodes a protein of 66 amino acid residues, which displays at least 74% identity with Csp proteins of Lactobacillus genera. Northern blot analysis revealed that cspA is transcribed monocistronically and that its expression is induced after a temperature downshift from 37 degrees C to 20 degrees C. The transcriptional start site has been determined and is situated 98 bp upstream of the initiation codon. A cspA mutant strain was constructed and it showed reduced growth rate compared with the wild type at both optimal and low temperatures, demonstrating that CspA plays an important role in the physiology of L. casei.

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“…(Chapot-Chartier et al 1997;Kim et al 1997Mayo et al 1997;Francis et al 1998;Wouters et al 1998;Ferretti et al 2001 ). As pointed out previously, the number of CSP genes may vary between subspecies and strains (Champomier-Verges et al 2002).…”

Section: Adaptive Response Of Lab To Temperature Downshiftmentioning

confidence: 88%

Lactic Acid Bacteria: Genetics, Metabolism and Applications

2002

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Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene

Cited by 29 publications

References 38 publications

Physiological and Regulatory Effects of Controlled Overproduction of Five Cold Shock Proteins of Lactococcus lactis MG1363

Physiological and Regulatory Effects of Controlled Overproduction of Five Cold Shock Proteins of Lactococcus lactis MG1363

Cloning and characterization of a gene encoding a cold-shock protein inLactobacillus casei

Lactic Acid Bacteria: Genetics, Metabolism and Applications

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