The Coexistence of Multiple Bluetongue Virus Electropherotypes in Individual Cattle during Natural Infection

Oberst, Richard D.; Squire, Kevin; Stott, Jeffrey L; Chuang, Ronald Y.; Osburn, Bennie

doi:10.1099/0022-1317-66-9-1901

Cited by 36 publications

(21 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This has been shown to occur experimentally in dually infected ruminants. 17 Our results differ from those of Oberst et al 16 in that the double-banded electropherotype reported here was stable over a period of 4 years. In the present study, single-banded variants of the doublebanded electropherotypes could only be isolated after extensive serial plaque purifications, and reassortant strains could not be demonstrated in ruminants.…”

Section: Discussioncontrasting

confidence: 57%

“…3 It has been shown that viral strains of a single serotype, exhibiting more than 1 electropherotype, may coexist within a single animal or herd. 3,16 In addition, predominant electropherotypes have been shown to change during the course of a single bluetongue outbreak. 3 We report here the genetic transitions of BTV Received for publication May 20, 1988. infecting domestic ruminants in eastern Colorado over a 4-year period and the continued predominance of an electropherotype apparently resulting from multiple infections.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Genotypic Transitions among Bluetongue Viral Isolates from Domestic Ruminants in Colorado during 1981–1984

et al. 1989

View full text Add to dashboard Cite

Abstract. Two predominant electropherotypes of bluetongue virus (BTV) serotype 11 isolates from cattle during a 198l-l984 field study in eastern Colorado were characterized. The genomes of strains isolated from the first 2 years of the study had 1 predominant electropherotype (CO81), with the exception of 1 isolate that differed only in the migration of segment 3. A second electropherotype (CO83), with differences in the migration of 4 segments, coexisted in the same region during 1983 and 1984 with strains having the CO81 RNA profile. The genomes of CO81 and CO83 were also distinguishable from those of the US prototype of BTV 11. Analysis of the polypeptides of representative strains of each electropherotype by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the proteins were very similar. The occurrence of the CO81 electropherotype was apparently the result of multiple viral infections since the positions of 7 segments had faint second bands and single-banded variants were isolated after serial plaque purifications. In addition, protein 7 of 1 of the CO81 isolates and protein 7 of the single-banded variant differed as shown by reverse phase-high performance liquid chromatography of 35 S-methionine-labeled tryptic peptides.Bluetongue virus (BTV), an arthropod-borne virus replicating in both Culicoides vectors and ruminant hosts, causes a disease characterized by fever, inflammation, and congestion of oral and nasal mucous membranes. Sheep, in particular, may suffer from a severe, fatal form of the disease; in cattle and goats, bluetongue is generally subclinical, and virus can frequently be isolated from apparently healthy animals. 2 There are 5 known serotypes of BTV in the US: 2, 10, 11, 13, and 17. 1,7 In nature, more than 1 serotype has been isolated from the blood of a single anima1. 20 Serological data have been expanded by genomic analysis of BTV isolates. 3,8,12,18 Genomic variation among naturally occurring BTV, as with other members of the family Reoviridae, has been demonstrated by electrophoretic separation of the 10 double-stranded RNA genomic segments. Concurrent polypeptide analysis has also established translational significance of observed genomic differences. 3 It has been shown that viral strains of a single serotype, exhibiting more than 1 electropherotype, may coexist within a single animal or herd. 3,16 In addition, predominant electropherotypes have been shown to change during the course of a single bluetongue outbreak. 3 We report here the genetic transitions of BTV Received for publication May 20, 1988. infecting domestic ruminants in eastern Colorado over a 4-year period and the continued predominance of an electropherotype apparently resulting from multiple infections. Materials and methodsViruses. Blood samples from ruminants were collected yearly from May through September 198l-l984. Bluetongue virus field strains were isolated from heparinized blood collected from individual cattle, sheep, and goats. Isolations were made by intravascular inoculation of 1...

show abstract

Section: Discussioncontrasting

confidence: 57%

mentioning

confidence: 99%

Genotypic Transitions among Bluetongue Viral Isolates from Domestic Ruminants in Colorado during 1981–1984

et al. 1989

View full text Add to dashboard Cite

show abstract

“…Thus, an individual fly could become mixedly infected with different BTV strains by sequential feeding on hosts viraemic for different viruses, or by feeding on a single mixedly infected vertebrate host. The feasibility of either mode of acquiring a mixed infection is supported by the presence of multiple BTV serotypes in many infected herds (Osburn et al, 1981) and the recovery of multiple serotypes from individual infected sheep and cattle (Stott et al, 1982;Oberst et al, 1985). The genetic consequence of mixed BTV infection of C. variipennis is not known.…”

confidence: 99%

Mixed Infection of Culicoides variipennis with Bluetongue Virus Serotypes 10 and 17: Evidence for High Frequency Reassortment in the Vector

Samal

el-Hussein

Holbrook³

et al. 1987

Journal of General Virology

View full text Add to dashboard Cite

“…This has previously been described for bluetongue virus (Squire et al, 1983;Oberst et al, 1985;Knudson et al, 1982).…”

Section: All Of the Zimbabwe Isolates Cross-reacted With Each Other Imentioning

confidence: 99%

Characterization of Potentially Foetotropic Palyam Serogroup Orbiviruses Isolated in Zimbabwe

Whistler

Swanepoel

1988

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYTwelve Palyam serogroup orbiviruses isolated in Zimbabwe from aborted cattle foetuses, plus one isolated from the visceral organs of a cow and another from vulture faeces, were examined in comparison with known members of the serogroup by complement fixation, indirect immunofluorescence, fluorescent focus reduction neutralization tests and PAGE of the segmented, dsRNA genomes. The viruses were indistinguishable from known members of the serogroup by complement fixation and indirect immunofluorescence, but two novel viruses, for which the names Gweru and Marondera are proposed, and two previously described viruses, Nyabira and Abadina, were identified by neutralization tests. The dsRNA profiles of Abadina serotype isolates differed from that of the Abadina prototype virus, indicating that different electropherotypes may occur within serotypes.

show abstract

The Coexistence of Multiple Bluetongue Virus Electropherotypes in Individual Cattle during Natural Infection

Cited by 36 publications

References 33 publications

Genotypic Transitions among Bluetongue Viral Isolates from Domestic Ruminants in Colorado during 1981–1984

Genotypic Transitions among Bluetongue Viral Isolates from Domestic Ruminants in Colorado during 1981–1984

Mixed Infection of Culicoides variipennis with Bluetongue Virus Serotypes 10 and 17: Evidence for High Frequency Reassortment in the Vector

Characterization of Potentially Foetotropic Palyam Serogroup Orbiviruses Isolated in Zimbabwe

Contact Info

Product

Resources

About