The clpX gene plays an important role in bacterial attachment, stress tolerance, and virulence in Xanthomonas campestris pv. campestris

Lo, Hsueh-Hsia; Liao, Chao-Tsai; Li, Chih-En; Chiang, Ying-Chuan; Hsiao, Yi‐Min

doi:10.1007/s00203-019-01772-3

Cited by 16 publications

(18 citation statements)

References 44 publications

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“…Our data show that the Clp chaperone-protease genes are required for the bacterium to cause disease and thrive within the mammalian host. Like what has been observed in other bacterial pathogens [35][36][37], we have uncovered several specific roles for the A. baumannii Clp chaperone-protease system, including intracellular survival and biofilm formation. We confirmed that the defects observed in A. baumannii clp mutants after interaction with phagocytes or biofilm formation are not caused by aberrant growth phenotypes under standard laboratory conditions.…”

Section: Discussionsupporting

confidence: 67%

Acinetobacter baumannii Strains Deficient in the Clp Chaperone-Protease Genes Have Reduced Virulence in a Murine Model of Pneumonia

et al. 2021

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Acinetobacter baumannii has emerged as a significant opportunistic Gram-negative pathogen and causative agent of nosocomial pneumonia especially in immunocompromised individuals in intensive care units. Recent advances to understand the contribution and function of A. baumannii virulence factors in its pathogenesis have begun to elucidate how this bacterium interacts with immune cells and its interesting mechanisms for multi-antibiotic resistance. Taking advantage of the availability of the A. baumannii AB5075 transposon mutant library, we investigated the impact of the A. baumannii Clp genes, which encode for a chaperone-protease responsible for the degradation of misfolded proteins, on bacterial virulence in a model of pneumonia using C57BL/6 mice and survival within J774.16 macrophage-like cells. Clp-protease A. baumannii mutants exhibit decreased virulence in rodents, high phagocytic cell-mediated killing and reduced biofilm formation. Capsular staining showed evidence of encapsulation in A. baumannii AB5075 and Clp-mutant strains. Surprisingly, clpA and clpS mutants displayed irregular cell morphology, which may be important in the biofilm structural deficiencies observed in these strains. Interestingly, clpA showed apical-like growth, proliferation normally observed in filamentous fungi. These findings provide new information regarding A. baumannii pathogenesis and may be important for the development of therapies intended at reducing morbidity and mortality associated with this remarkable pathogen.

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Section: Discussionsupporting

confidence: 67%

Acinetobacter baumannii Strains Deficient in the Clp Chaperone-Protease Genes Have Reduced Virulence in a Murine Model of Pneumonia

et al. 2021

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“…The ClpXP protease recycles misfolded proteins in the cytoplasm ( 86 ), and its inactivation in X. campestris pv. campestris through clpX or clpP mutations leads to pleiotropic effects, including increased stress sensitivity ( 87 , 88 ). The clpX and clpP genes were also critical in planta in an R. solanacearum Tn-seq study ( Table 4 ) ( 32 ).…”

Section: Resultsmentioning

confidence: 99%

A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

et al. 2022

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Xanthomonas hortorum was recently the subject of renewed interest, as several studies highlighted that its members were responsible for diseases in a wide range of plant species, including crops of agricultural relevance (e.g., tomato and carrot). Among X. hortorum variants, X. hortorum pv. vitians is a reemerging foliar hemibiotrophic phytopathogen responsible for severe outbreaks of bacterial leaf spot of lettuce all around the world.

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“…The method of temperature tolerance assay was as described earlier (Lo et al 2020). Brie y, overnight cultures of tested strains were diluted with fresh LB broth to an initial density of OD 550 = 0.5 (5 × 10 8 cells/mL) and followed tenfold serial dilutions (5 × 10 7 to 5 × 10 5 cells/mL).…”

Section: Temperature Tolerance Assaymentioning

confidence: 99%

“…The X. campestris pv. campestris clpX was found to play an important role in bacterial attachment, stress tolerance, and virulence (Lo et al 2020). The X. campestris pv.…”

Section: Introductionmentioning

confidence: 99%

Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris

Chang

Liao

et al. 2022

Antonie van Leeuwenhoek

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ATP-dependent proteases (FtsH, Lon, and Clp family proteins) are ubiquitous in bacteria and play essential roles in many regulatory cell processes. Xanthomonas campestris pv. campestris is a Gramnegative pathogen and can cause black rot diseases in crucifers. The genome of X. campestris pv. campestris have several clp genes, namely clpS, clpA, clpX, clpP, clpQ, and clpY. Among them, only clpX and clpP were known to be required for pathogenicity. Here, we focused on two uncharacterized clp genes (clpS and clpA) that encode the adaptor (ClpS) and ATPase subunit (ClpA) of the ClpAP protease complex, respectively. Transcriptional analysis revealed that expression of both clpS and clpA is induced by heat shock. Inactivation of clpA but not clpS resulted in susceptibility to high temperature and revealed an attenuation of virulence on the host plant. The altered phenotypes of the clpA mutant could be complemented in trans. Site-directed mutagenesis revealed that K223 and K504 are critical amino acid residues for ClpA function in heat tolerance. The clpA mutant revealed different protein expression pro les as compared to the wild type in response to heat stress. Taken together, we characterized two clp genes (clpS and clpA) by examining their expression and function including stress tolerance and pathogenicity. We demonstrated that both clpS and clpA are expressed in a temperature dependent manner, and clpA is required for survival at high temperature and full virulence of X. campestris pv. campestris. This is the rst time that clpS and clpA has been characterized in Xanthomonas.

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The clpX gene plays an important role in bacterial attachment, stress tolerance, and virulence in Xanthomonas campestris pv. campestris

Cited by 16 publications

References 44 publications

Acinetobacter baumannii Strains Deficient in the Clp Chaperone-Protease Genes Have Reduced Virulence in a Murine Model of Pneumonia

Acinetobacter baumannii Strains Deficient in the Clp Chaperone-Protease Genes Have Reduced Virulence in a Murine Model of Pneumonia

A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris

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