A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv.
            <i>vitians</i>

Morinière, Lucas; Mirabel, Laurène; Gueguen, Erwan; Bertolla, Franck

doi:10.1128/msystems.01290-21

Cited by 11 publications

(5 citation statements)

References 115 publications

(195 reference statements)

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“…Tn-seq is a powerful method that has successfully been used to characterize essential genes in various conditions and many different species. For example, it has been used to identify essential genes for human gut colonization of Bacteroides thetaiotaomicron (16), mouse colonization by human pathogens such as Vibrio cholerae, Pseudomonas aeruginosa, or Streptococcus pneumoniae (14,17,18), plant colonization by phytopathogens (19)(20)(21), tobramycin resistance genes of P. aeruginosa (22), toxic compound resistance genes in P. putida (23) and identification of genetic targets for improved tolerance of P. putida towards compounds relevant to lignin conversion (24). This technique has also been also employed to identify gold, silver and copper resistance genes in Burkholderia cenocepacia (25,26).…”

Section: Introductionmentioning

confidence: 99%

High-throughput Tn-seq screens identify both known and novelPseudomonas putidaKT2440 genes involved in metal resistance

Royet,

Kergoat,

Lutz

et al. 2024

Preprint

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Chemical waste with toxic effects is released into the environment by industrial and urban activities.Pseudomonas putida, a rhizosphere bacterium, harbors a wide variety of genes capable of degrading hydrocarbons and xenobiotic compounds in its natural environment. This bacterium harbors also a large set of metal resistance genes. Most studies that identify genes involved in metal resistance inP. putidafocus on over/underexpressed genes and may miss other genes important for metal resistance whose expression does not change. In this study, we used a Tn-seq approach to determine the essential genome ofP. putidarequired for growth in the presence of an excess of metals in a culture medium. Tn-seq enables the detection of mutants with reduced or increased fitness in the presence of metal excess. We validated our screen by identifying known metal resistance gene such asczcA-1(PP_0043),cadA-3(PP_5139),cadR(PP_5140) andpcoA2(PP_5380). Their mutants were underrepresented in the presence of zinc, cadmium (forcadA-3andcadR) or copper respectively. In this study, we demonstrate by targeted mutagenesis and complementation assay thatPP_5337andPP_0887are putative transcriptional regulators involved in copper and cadmium resistance, respectively, inP. putida. The study revealed the role of two genes,PP_1663andPP_5002, in cadmium and cobalt resistance respectively. This is the first evidence linking these genes to metal resistance and highlights the incomplete understanding of metal resistance mechanisms inP. putida.

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Section: Introductionmentioning

confidence: 99%

High-throughput Tn-seq screens identify both known and novelPseudomonas putidaKT2440 genes involved in metal resistance

Royet,

Kergoat,

Lutz

et al. 2024

Preprint

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“…Transposon-sequencing (Tn-seq) identifies insertional mutations that confer an advantage or a defect under a given condition. In several plant pathogens, Tn-seq unveiled genes involved in the competitive colonization of lesions caused by necrotrophic and biotrophic pathogens ( Duong et al., 2018 ; Helmann et al., 2019 ; Royet et al., 2019 ; Su et al., 2021 ; Torres et al., 2021 ; Helmann et al., 2022 ; Luneau et al., 2022 ; Morinière et al., 2022 ). Tn-seq analyses revealed a large set of genes associated with the colonization of lesions provoked by D. dadantii on chicory leaves and by D. dianthicola and D. dadantii on potato tubers ( Royet et al., 2019 ; Helmann et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Dissimilar gene repertoires of Dickeya solani involved in the colonization of lesions and roots of Solanum tuberosum

Robic

Munier²,

Effantin

et al. 2023

Front. Plant Sci.

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Dickeya and Pectobacterium species are necrotrophic pathogens that macerate stems (blackleg disease) and tubers (soft rot disease) of Solanum tuberosum. They proliferate by exploiting plant cell remains. They also colonize roots, even if no symptoms are observed. The genes involved in pre-symptomatic root colonization are poorly understood. Here, transposon-sequencing (Tn-seq) analysis of Dickeya solani living in macerated tissues revealed 126 genes important for competitive colonization of tuber lesions and 207 for stem lesions, including 96 genes common to both conditions. Common genes included acr genes involved in the detoxification of plant defense phytoalexins and kduD, kduI, eda (=kdgA), gudD, garK, garL, and garR genes involved in the assimilation of pectin and galactarate. In root colonization, Tn-seq highlighted 83 genes, all different from those in stem and tuber lesion conditions. They encode the exploitation of organic and mineral nutrients (dpp, ddp, dctA, and pst) including glucuronate (kdgK and yeiQ) and synthesis of metabolites: cellulose (celY and bcs), aryl polyene (ape), and oocydin (ooc). We constructed in-frame deletion mutants of bcsA, ddpA, apeH, and pstA genes. All mutants were virulent in stem infection assays, but they were impaired in the competitive colonization of roots. In addition, the ΔpstA mutant was impaired in its capacity to colonize progeny tubers. Overall, this work distinguished two metabolic networks supporting either an oligotrophic lifestyle on roots or a copiotrophic lifestyle in lesions. This work revealed novel traits and pathways important for understanding how the D. solani pathogen efficiently survives on roots, persists in the environment, and colonizes progeny tubers.

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“…Such TnSeq approaches have been widely used to study animal pathogens and have led to the discovery of infectious processes including virulence factors, genes required for transmission between hosts or antibiotic resistance (reviewed in Cain et al ., 2020; van Opijnen & Levin, 2020) but have only recently been applied to plant pathogens. To date, TnSeq screens of genes contributing to in planta fitness have been performed in Pantoea stewartii (on corn; Duong et al ., 2018), Dickeya dadantii (on chicory; Royet et al ., 2019), Agrobacterium tumefaciens (on tomato; Gonzalez‐Mula et al ., 2019; Torres et al ., 2022), Pseudomonas syringae (on bean and pepper; Helmann et al ., 2019, 2020), Ralstonia solanacearum (on tomato; Su et al ., 2021) and Xanthomonas hortorum (on lettuce; Morinière et al ., 2022). While these studies provided diverse insights specific to each pathosystem, they all highlighted the importance of metabolic capacities and secretion systems for optimal growth in planta .…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

et al. 2022

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Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages.Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media.Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation.This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.

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A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

Cited by 11 publications

References 115 publications

High-throughput Tn-seq screens identify both known and novelPseudomonas putidaKT2440 genes involved in metal resistance

High-throughput Tn-seq screens identify both known and novelPseudomonas putidaKT2440 genes involved in metal resistance

Dissimilar gene repertoires of Dickeya solani involved in the colonization of lesions and roots of Solanum tuberosum

Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

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