The Clinicopathological Effects of the Double Immunization with Formalized Killed Vaccine against Pasteurella multocida Challenge in Rabbits

Abstract

The disease caused by Pasteurella multocida is an extremely common and difficult issue of rabbits utilized for biomedical research. The vaccination techniques are dependably the most powerful preventive measures. In the present study, 30 New Zealand rabbits (1.5 kg average body weight and 6-8 weeks old) were divided into 3 groups, control group (I), challenged nonvaccinated group (II), and challenged double vaccinated group (III). 1 st dose was administered at the 1 st day followed by similar booster dose after 21 days. At the end of the 2 nd , 4 th , and 6 th weeks of the experiment, blood samples were collected from the ear vein for hematological, plasma, and serum examination. At the end of the 6 th week, the rabbits were anaesthetized and sacrificed to collect the tissue specimens from liver, kidneys, spleen, lungs and heart for histopathological study. The results showed that double immunization with killed vaccine of P. multocida increased the immune response of the animals and the leukocyte phagocytic activity against P. multocida and also improved the clinicopathological and histopathological findings. Taken together, our findings proved that double immunization with killed vaccine of P. multocida increased the phagocytic activity of the immune cells and the immune status of animals against infection.

Histopathological Effects of using Grape Seed Extract Alone or in Combination with Ofloxacin against Pasteurella Multocida in Rabbits

“…Alveolar collapse, tissue destruction and compensatory emphysema. Our results were in agreement with (Mohamed et al, 2019) who proved that lung of Pasteurella infected rabbit revealed extensive hemorrhage with presence of large numbers of extravasated RBCs in the alveolar lumens. (Alam et al, 2018) observed that the lung tissue of normal control rabbits exhibited normal bronchial and bronchiolar structures with normal alveolar and perialveolar capillaries.…”

“…On the other hand, spleen of infected non treated group showed severe congestion and focal depletion of white pulp. our study was in agreement of (Mohamed et al, 2019) who observed severe lymphoid depletion of the lymphoid follicle associated with hemosiderosis. Spleen showed necrosis, edema and lymphoid depletion of lymphoid follicles of the white pulp (El-Demerdash et al, 2015).…”

“…Heart of control group showed normal heart muscles while heart of group II showed severe congestion of blood vessels and interstitial inflammatory reaction, degeneration and intermuscular edema. (Mohamed et al, 2019) recorded extensive hemorrhages, intermuscular edema, with vacuolation of the cardiomyocytes in heart of Pasteurella infected group. (Wada et al, 2010) reported mild to moderate congestion of blood vessels and hemorrhage in the intermycium of the heart in cases of fowl cholera.…”

“…Hepatic cords were arranged in radiating manner around the central veins and had normal hexagonal hepatic cells with abundant eosinophilic cytoplasm. (Mohamed et al, 2019) proved that liver of Pasteurella infected rabbit showed peliosis and hepatitis (multiple, randomly distributed, bloodfilled cavities throughout the liver. In our study, liver of group II revealed severe congestion of hepatic blood vessels, hyperplasia of bile ducts, and massive lymphocytic infiltration along with fibrosis around the hepatic area.…”

“…Group II showed degeneration of renal tubules, proliferation of some glomeruli and focal interstitial aggregations with lymphocytes. vascular congestion and severe hydropic degeneration in the epithelial lining of the tubular epithelium of kidney were recorded (Mohamed et al, 2019).…”