The clinical significance and potential molecular mechanism of integrin subunit beta 4 in laryngeal squamous cell carcinoma

Zhong, Feng; Lu, Huiping; Chen, Gang; Dang, Yi‐Wu; Li, Guosheng; Chen, Xiaoyi; Qin, Yong‐Ying; Ying, Yang; Zhang, Xiao-Guohui; Liang, Yao; Li, Mingxuan; Mo, Miao; Zhang, Kai-Lang; Ding, Hua; Huang, Zhi‐Guang; Wei, Zhu‐Xin

doi:10.1016/j.prp.2019.152785

Cited by 12 publications

(9 citation statements)

References 40 publications

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“…Sopita et al demonstrated that ITGB4 was expressed in chondrocyte sheets (Wongin et al, 2020). ITGB4 also regulates ECM that exerts a crucial role in the pathogenesis of OA (Zhong et al, 2020). In addition, we found that miR-302d-3p, directly targeting ITGB4, was expressed with higher levels in OA compared with normal controls.…”

Section: Discussionsupporting

confidence: 56%

Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Sun

Zheng

Liu

et al. 2021

Front. Mol. Biosci.

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Background: Osteoarthritis (OA) is one of the most common degenerative joint diseases characterized by increased apoptosis and autophagy deficiency. The investigation was performed to examine the effect of valproic acid (VPA) and molecular mechanism related to miR-302d-3p/ITGB4 axis in OA.Methods: The OA clinical samples were obtained from the GEO database to analyze differentially expressed genes. An in vitro OA model was mimicked by LPS in CHON-001 cells. Autophagy-related genes were downloaded from the HADb website, and potential drugs were mined using the CTD website. The upstream factors of ITGB4 were predicted with bioinformatics analysis, which was validated by luciferase activity assay and RIP assay. Cell viability and apoptosis were evaluated using CCK-8 and flow cytometry. The expression levels, including ITGB4, miR-302d-3p, and autophagy-/PI3K-AKT pathway-related markers, were measured by qRT-PCR or/and western blot.Results: Our results showed that miR-302d-3p inhibited cell viability and promoted apoptosis of LPS-treated CHON-001 cells by targeting ITGB4. VPA treatment remarkably alleviated LPS-stimulated injury in CHON-001 cells. The inhibitory effect of VPA on LPS-stimulated damage in CHON-001 cells was weakened by miR-302d-3p overexpression, while it was intensified because of ITGB4 upregulation. Mechanistically, VPA treatment induced a significant decrease in the levels of p-PI3K and p-AKT in LPS-stimulated CHON-001 cells through regulating miR-302d-3p/ITGB4 axis.Conclusion: Overall, VPA treatment may ameliorate LPS-induced injury on chondrocytes via the regulation of miR-302d-3p/ITGB4 pair and the inactivation of the PI3K-AKT pathway.

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Section: Discussionsupporting

confidence: 56%

Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Sun

Zheng

Liu

et al. 2021

Front. Mol. Biosci.

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“…Furthermore, the summary receiver operating characteristic (sROC) curve was used to distinguish TC tissues from non-cancerous tissues, which were gathered from the above databases. Sensitivity and specificity, as well as the corresponding likelihood ratio (LR) and diagnostic odds ratio (DOR) were calculated using meta-disk Version 1.4 [ 28 – 30 ].…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical basigin expression level in thyroid cancer tissues

et al. 2020

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Background: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. Methods: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays, and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. Results: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly upregulated in TC cases. Conclusion: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions.

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“…Furthermore, the summary receiver operating characteristic (sROC) curve was used to distinguish TC tissues from noncancerous tissues, which were gathered from the above databases. Sensitivity and speci city, as well as the corresponding likelihood ratio (LR) and diagnostic odds ratio (DOR) were calculated using meta-disc Version 1.4 [28][29][30].…”

Section: Comprehensive Analysis Of Bsg Expression In Tcmentioning

confidence: 99%

Immunohistochemical Basigin Expression Level in Thyroid Cancer Tissues

Guo

Tang

Pang

et al. 2020

Preprint

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Background: Thyroid cancer (TC) is the most common endocrine malignancy, Basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. Methods: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. Results: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p<0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly up-regulated in TC cases. Conclusion: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions.

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The clinical significance and potential molecular mechanism of integrin subunit beta 4 in laryngeal squamous cell carcinoma

Cited by 12 publications

References 40 publications

Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Immunohistochemical basigin expression level in thyroid cancer tissues

Immunohistochemical Basigin Expression Level in Thyroid Cancer Tissues

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