Immunohistochemical basigin expression level in thyroid cancer tissues

Abstract

Background: Thyroid cancer (TC) is the most common endocrine malignancy; basigin (also known as BSG) plays a crucial role in tumor cell invasion, metastasis, and angiogenesis. This study was designed to identify the change of BSG expression in TC and its possible potential mechanism. Methods: The BSG expression levels in TC were demonstrated using data collected from in-house immunohistochemical (IHC), RNA-sequencing (RNA-seq), microarrays, and literatures. Integrated analysis was performed to determined BSG expression levels in TC comprehensively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed with the integration of BSG co-expressed genes and differentially expressed genes (DEGs) in TC tissues to explore the potential mechanisms of BSG in TC. Results: The protein expression level of BSG was significantly higher in TC cases based on the IHC experiments. In addition, the combined SMD for BSG expression was 0.39 (p < 0.0001), the diagnostic odds ratio was 3.69, and the AUC of the sROC curve was 0.6986 using 1182 TC cases and 437 non-cancerous cases from 17 independent datasets. Furthermore, BSG co-expressed genes tended to be enriched in gene terms of the extracellular matrix (ECM), cell adhesion, and cell-cell interactions. The expression levels of nine hub BSG co-expressed genes were markedly upregulated in TC cases. Conclusion: BSG expression levels were closely correlated with the progression of TC and may affect the signals of the ECM, cell adhesion, and cell-cell interactions.

Predicting factors of central lymph node metastasis and BRAFV600E mutation in Chinese population with papillary thyroid carcinoma

“…With the emerging understanding of molecular genetics in thyroid cancer, several specific molecular events in PTC have been identified that assist in the diagnosis and prognostic evaluation of PTC patients [11][12][13]. The Btype Raf kinase (BRAF) V600E mutation was found to be the most important candidate biomarker for PTC diagnosis because of its high prevalence and high specificity [14,15].…”

Defining Specific Cell States of MPTP-Induced Parkinson’s Disease by Single-Nucleus RNA Sequencing

“…We simulated the activation trajectory of ENDO cells and detected the specific deletions of reactive ENDO in PD, especially in the subset of cells of Bsg High . Bsg plays a crucial role in angiogenesis, and its co-expressed genes tended to be enriched in gene terms of the extracellular matrix, cell adhesion and cell-cell interactions [66]. We hypothesized that the loss of ENDO cells in PD resulted in the destruction of tight junctions between cells, thus enhancing the permeability of BBB and promoting the entry of MPP + toxins into the brain environment.…”

Defining specific cell states of MPTP-induced Parkinson’s disease by single-nucleus RNA sequencing

“…We simulated the activation trajectory of ENDO cells and detected the specific deletions of reactive ENDO in PD, especially in the subset of cells of Bsg High . Bsg plays a crucial role in angiogenesis, and its co-expressed genes tended to be enriched in gene terms of the extracellular matrix, cell adhesion, and cell-cell interactions 67 . We hypothesized that the loss of ENDO cells in PD resulted in the destruction of tight junctions between cells, thus enhancing the permeability of BBB and promoting the entry of MPP + toxins into brain environment.…”