The Clinical Potential of Oral Microbiota as a Screening Tool for Oral Squamous Cell Carcinomas

Zhou, Xinxuan; Yu, Hao; Peng, Xian; Li, Bolei; Han, Qi; Ren, Biao; Li, Mingyun; Li, Longjiang; Li, Yang; Cheng, Guo; Li, Jiyao; Ma, Yue; Cheng, Lei

doi:10.3389/fcimb.2021.728933

Cited by 26 publications

(31 citation statements)

References 51 publications

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“… 87 , 88 Microbiome sequencing was used to evaluate the bacterial communities associated with OSCC, and it was found that the diversity and relative abundance of bacteria in the saliva of OSCC patients changed significantly. 74 , 89 When used as a diagnostic marker, oral bacteria were found to have 80% sensitivity and 83% specificity between the case group and the control group. 74 …”

Section: Oral Bacteria and Cancersmentioning

confidence: 99%

“… 74 , 89 When used as a diagnostic marker, oral bacteria were found to have 80% sensitivity and 83% specificity between the case group and the control group. 74 …”

Section: Oral Bacteria and Cancersmentioning

confidence: 99%

“… 71 – 73 The oral microbes that play a role in this can be used as potential biomarkers for the study of oral cancers and used to detect the development of oral cancer. 74 , 75 An in-depth understanding of the interaction mechanism between oral microbes and tumors will facilitate the design of subsequent new targeted drugs. This will play a great role in the diagnosis, treatment, and prognosis of patients with oral cancer in the future.…”

Section: Oral Bacteria and Cancersmentioning

confidence: 99%

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Oral microbiota in human systematic diseases

et al. 2022

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Oral bacteria directly affect the disease status of dental caries and periodontal diseases. The dynamic oral microbiota cooperates with the host to reflect the information and status of immunity and metabolism through two-way communication along the oral cavity and the systemic organs. The oral cavity is one of the most important interaction windows between the human body and the environment. The microenvironment at different sites in the oral cavity has different microbial compositions and is regulated by complex signaling, hosts, and external environmental factors. These processes may affect or reflect human health because certain health states seem to be related to the composition of oral bacteria, and the destruction of the microbial community is related to systemic diseases. In this review, we discussed emerging and exciting evidence of complex and important connections between the oral microbes and multiple human systemic diseases, and the possible contribution of the oral microorganisms to systemic diseases. This review aims to enhance the interest to oral microbes on the whole human body, and also improve clinician’s understanding of the role of oral microbes in systemic diseases. Microbial research in dentistry potentially enhances our knowledge of the pathogenic mechanisms of oral diseases, and at the same time, continuous advances in this frontier field may lead to a tangible impact on human health.

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Section: Oral Bacteria and Cancersmentioning

confidence: 99%

“… 74 , 89 When used as a diagnostic marker, oral bacteria were found to have 80% sensitivity and 83% specificity between the case group and the control group. 74 …”

Section: Oral Bacteria and Cancersmentioning

confidence: 99%

Section: Oral Bacteria and Cancersmentioning

confidence: 99%

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Oral microbiota in human systematic diseases

et al. 2022

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“…However, a wide heterogeneity was observed which made it impossible to perform an advanced statistical analysis. Eight studies were case–control [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ], two were cohort studies [ 35 , 36 ], and one was a cross-sectional cohort study [ 37 ]. Of the eleven studies, five were from Taiwan [ 30 , 34 , 35 , 36 , 37 ], three were from China [ 28 , 29 , 32 ], one was from the USA [ 27 ], one was from Sudan [ 31 ], and one was from Japan [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Salivary Microbiota Composition in Patients with Oral Squamous Cell Carcinoma: A Systematic Review

Mauceri

Coppini

Vacca

et al. 2022

Cancers

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Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers worldwide. Despite recent advances in diagnosis and treatment, in recent years, an increase in the incidence of OSCC has been registered, and the mortality rate is still high. This systematic review aims to identify a potential association between the composition of salivary microbiota and OSCC. Materials and Methods: The protocol for this study was designed following the PRISMA guidelines. Records were identified using different search engines (e.g., Medline/PubMed). Observational studies, in human subjects with histological diagnosis of OSCC, concerning the analysis of salivary microbiota, were selected. Results: Eleven papers were included. The salivary microbiomes of 1335 patients were analysed (n.687 OSCC and n.648 controls). Due to the great heterogeneity of the studies, it was not possible to profile a specific microbiota associated with OSCC. However, periodontal pathogens were the most common bacteria detected in patients with OSCC (i.e., Fusobacterium, Prevotella). Conclusions: Although there are evident alterations in the salivary microbiota composition in OSCC patients, it is still a challenge to identify a specific microbiota pattern in OSCC patients. If the associations between specific salivary microorganisms and OSCC are confirmed, microbiome analysis could be a useful tool for the screening and follow-up of patients affected by OSCC.

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“…Previous methods have either assessed biomolecules from the human side (e.g. CD44 protein [39] or RNA – 6 markers that include interleukins IL-1Beta, IL-8, OAZ1SAT1S100P, and DUSP1 [40] or difference in species with 16S rRNA gene sequencing and metagenomics [41] [42], but their results are less promising, and while they have reported discovery results, they have not been validated in independent cohorts. Large independent prospective studies are required to advance adoption of the new adjunctive technologies in clinical and eventually in home-based testing settings.…”

Section: Discussionmentioning

confidence: 99%

Detecting salivary host-microbiome RNA signature for aiding diagnosis of oral and throat cancer

Banavar

Ogundijo

Azcona

et al. 2022

Preprint

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Objectives Oral squamous cell carcinoma (OSCC) and oropharyngeal squamous cell carcinoma (OPSCC) are the two major subtypes of head and neck cancer (HNC) that can go undetected resulting in late detection and poor outcomes. We describe the development and validation of a convenient and easy-to-use test, called CancerDetect for Oral & Throat cancerTM (CDOT), to detect markers of OSCC and/or OPSCC within a high-risk population using salivary metatranscriptomics. Materials and Methods We collected saliva samples from 1,175 unique individuals who were 50 years or older, or adults who had a history of tobacco use. All saliva samples were processed through a metatranscriptomic method to isolate microbial organisms and functions, as well as human transcripts. Of the 1175 samples, 945 were used to train a classifier using machine learning methods, resulting in a salivary RNA metatranscriptomic signature. The classifier was then independently validated on the 230 remaining samples unseen by the classifier, consisting of 20 OSCC (all stages), 76 OPSCC (all stages), and 134 negatives (including 14 pre-malignant). Results On the validation cohort, the specificity of the CDOT test was 94%, sensitivity was 90% for participants with a histopathological diagnosis of OSCC, and 84.2% for participants with a diagnosis of OPSCC. Similar classification results were observed among people in early stage (stages I & II) vs late stage (stages III & IV) of OSCC and OPSCC. Conclusions CDOT is a non-invasive test that can be easily administered in dentist offices, primary care centers and specialized cancer clinics for early detection of OPSCC and OSCC. This test, having received breakthrough designation by the US Food and Drug Administration (FDA), will broadly enable early diagnosis of OSCC and OPSCC, saving lives and significantly reducing healthcare expenditure.

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The Clinical Potential of Oral Microbiota as a Screening Tool for Oral Squamous Cell Carcinomas

Cited by 26 publications

References 51 publications

Oral microbiota in human systematic diseases

Oral microbiota in human systematic diseases

Salivary Microbiota Composition in Patients with Oral Squamous Cell Carcinoma: A Systematic Review

Detecting salivary host-microbiome RNA signature for aiding diagnosis of oral and throat cancer

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