The Clinical Importance of Campylobacter concisus and Other Human Hosted Campylobacter Species

Liu, Fang; Ma, Ruonan; Wang, Yiming; Zhang, Li

doi:10.3389/fcimb.2018.00243

Cited by 92 publications

(97 citation statements)

References 239 publications

(228 reference statements)

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“…1 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, Sydney 2052, Australia. 2 Gastrointestinal and Liver Unit, Prince of Wales Hospital, University of New South Wales, Sydney, Australia.…”

Section: Supplementary Informationmentioning

confidence: 99%

“…Campylobacter species are fastidious Gram-negative, curved rod-shaped bacteria which require microaerobic to anaerobic conditions for growth [1]. Currently, the genus Campylobacter contains 40 species and subspecies [2]. While most Campylobacter species reside in the gastrointestinal tract of various animals as commensal bacterial species, some use humans as their natural host [2].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the genus Campylobacter contains 40 species and subspecies [2]. While most Campylobacter species reside in the gastrointestinal tract of various animals as commensal bacterial species, some use humans as their natural host [2].…”

Section: Introductionmentioning

confidence: 99%

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Analyses of energy metabolism and stress defence provide insights into Campylobacter concisus growth and pathogenicity

Yeow

Liu

et al. 2020

Gut Pathog

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Campylobacter concisus is an emerging enteric pathogen that is associated with inflammatory bowel disease. Previous studies demonstrated that C. concisus is non-saccharolytic and hydrogen gas (H 2) is a critical factor for C. concisus growth. In order to understand the molecular basis of the non-saccharolytic and H 2-dependent nature of C. concisus growth, in this study we examined the pathways involving energy metabolism and oxidative stress defence in C. concisus. Bioinformatic analysis of C. concisus genomes in comparison with the well-studied enteric pathogen Campylobacter jejuni was performed. This study found that C. concisus lacks a number of key enzymes in glycolysis, including glucokinase and phosphofructokinase, and the oxidative pentose phosphate pathway. C. concisus has an incomplete tricarboxylic acid cycle, with no identifiable succinyl-CoA synthase or fumarate hydratase. C. concisus was inferred to use fewer amino acids and have fewer candidate substrates as electron donors and acceptors compared to C. jejuni. The addition of DMSO or fumarate to media resulted in significantly increased growth of C. concisus in the presence of H 2 as an electron donor, demonstrating that both can be used as electron acceptors. Catalase, an essential enzyme for oxidative stress defence in C. jejuni, and various nitrosative stress enzymes, were not found in the C. concisus genome. Overall, C. concisus is inferred to have a non-saccharolytic metabolism in which H 2 is central to energy conservation, and a narrow selection of carboxylic acids and amino acids can be utilised as organic substrates. In conclusion, this study provides a molecular basis for the non-saccharolytic and hydrogen-dependent nature of C. concisus energy metabolism pathways, which provides insights into the growth requirements and pathogenicity of this species.

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Section: Supplementary Informationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analyses of energy metabolism and stress defence provide insights into Campylobacter concisus growth and pathogenicity

Yeow

Liu

et al. 2020

Gut Pathog

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“…Bacterial reads in aqueous humor and CSF, two fluids with very low endogenous RNA content that were collected in a sterile setting (and thus presumed to be sterile), most likely reflect background contamination during the workflow 29 . To illustrate the biological relevance of the bacterial signal, we looked into reads mapping to Campylobacter concisus, a gram-negative bacterium that is known to primarily colonize the human oral cavity, with some strains translocated to the intestinal tract 30 . We confirm the selective presence of reads mapping to Campylobacter concisus in saliva in both the small RNA and the mRNA data( Fig.…”

Section: Fig 4 Circrnas Are Enriched In Biofluids Compared To Tissuesmentioning

confidence: 99%

Charting extracellular transcriptomes in The Human Biofluid RNA Atlas

Hulstaert

Morlion

Cobos

et al. 2019

Preprint

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Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease.Where most studies focus on plasma or serum, other biofluids may contain more informative RNA molecules, depending on the type of disease. Here, we present an unprecedented atlas of messenger, circular and small RNA transcriptomes of a comprehensive collection of 20 different human biofluids. By means of synthetic spike-in controls, we compared RNA content across biofluids, revealing a more than 10 000-fold difference in RNA concentration. The circular RNA fraction is increased in nearly all biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. In addition, a subset of biofluids, including stool, sweat, saliva and sputum, contains high levels of bacterial RNAs. Our atlas enables a more informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Taken together, our results reveal novel insights in the RNA content of human biofluids and may serve as a valuable resource for future biomarker studies.

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“…The emerging Campylobacter species have been neglected but the integration of molecular techniques and suitable culture media in current diagnostic tests has helped in promoting the awareness of atypical species as relevant human and animal pathogens [5,22]. Campylobacter concisus has been associated with gastroenteritis, brain abscess, Crohn's disease, arthritis, and ulcerative colitis [14,[23][24]. Campylobacter hominis has been isolated in a blood sample of a septicemic patient [24], while C. gracilis has been associated with bacteremia, head infections, periodontitis, and empyema [23,26].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania

Gahamanyi

Mboera

Matee

et al. 2020

Preprint

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Background: A growing number of Campylobacter species other than C. jejuni and C. coli have been considered as emerging human and animal pathogens. However, the contribution of these species to human gastroenteritis is poorly documented. This study aimed at detecting Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania using Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene, and Sanger sequencing . Methods: A total number of 100 faecal samples (70 from human and 30 from cattle) were collected from diarrheic and non-diarrheic patients and healthy cattle in Kilosa district, Tanzania from July to October 2019. Species identification was conducted by PCR and 16S rRNA sequencing. The phylogenetic analysis was carried out by comparison of the 16S rRNA gene sequences to reference strains by the Neighbor-Joining method in MEGA X. Results: Campylobacter species detection rate by PCR was 65.7% (46/70) and 20% (6/30) in humans and cattle, respectively. There were five human diarrheic cases, four showed Campylobacter presence and two were from children ≤15 years of age. In humans, the 16S rRNA sequencing revealed that C. concisus was the most predominant species occurring at a frequency of 37.8% (14/37), followed by uncultured Campylobacter spp. 24.3% (9/37) and C. hominis 21.6% (8/37). The least represented species were C. jejuni and C. lanienae all occurring at 2.7% (1/37). In cattle, five (100%) sequenced PCR products matched with C. lanienae . Phylogenetic analysis revealed that Campylobacter 16S rRNA sequences were closely related to C. concisus , uncultured Campylobacter sp., C. hominis , and C. gracilis . Conclusion: The non- C. jejuni / C. coli species are present in human and cattle faecal samples and their true occurrence is probably under-reported due to shortcomings of conventional techniques used in most diagnostic microbiology laboratories. Based on our findings, we recommend that molecular techniques be adopted for direct detection of Campylobacter species during routine laboratory screening and surveillance studies. Keywords: Campylobacter , molecular diagnostics, polymerase chain reaction, sequencing, gastroenteritis, Tanzania

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The Clinical Importance of Campylobacter concisus and Other Human Hosted Campylobacter Species

Cited by 92 publications

References 239 publications

Analyses of energy metabolism and stress defence provide insights into Campylobacter concisus growth and pathogenicity

Analyses of energy metabolism and stress defence provide insights into Campylobacter concisus growth and pathogenicity

Charting extracellular transcriptomes in The Human Biofluid RNA Atlas

Molecular detection of Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania

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