The clinical implications of platelet-rich fibrin on periodontal regeneration: A systematic review

Madi, Marwa; Elakel, Ahmed M.

doi:10.1016/j.sdentj.2020.12.002

Cited by 3 publications

(1 citation statement)

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“…In a clinical setting, PRF represents an autologous source of growth factors, such as PDGF, VEGF, IGF, TGF-B, gradually released over a 10-day period [ 45 ]. In the clinic, its use has been successful in promoting hard and soft tissue regeneration [ 8 , 46 ]. Likewise, in the treatment of controlled periodontal disease, the combination of PRF with autologous osseous tissue reduces the pocket probing depth.…”

Section: Discussionmentioning

confidence: 99%

Viability and Adhesion of Periodontal Ligament Fibroblasts on a Hydroxyapatite Scaffold Combined with Collagen, Polylactic Acid–Polyglycolic Acid Copolymer and Platelet-Rich Fibrin: A Preclinical Pilot Study

et al. 2022

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Background: Conventional periodontal therapy relies on bone regeneration strategies utilizing scaffolds made of diverse materials, among which collagen, to promote cell adhesion and growth. Objective: To evaluate periodontal ligament fibroblast (HPdLF) cell adhesion and viability for periodontal regeneration purposes on hydroxyapatite scaffolds containing collagen (HAp-egg shell) combined with polylactic acid–polyglycolic acid copolymer (PLGA) and Platelet-Rich Fibrin (PRF). Methods: Four variations of the HAp-egg shell were used to seed HPdLF for 24 h and evaluate cell viability through a live/dead assay: (1) (HAp-egg shell/PLGA), (2) (HAp-egg shell/PLGA + collagen), (3) (HAp-egg shell/PLGA + PRF) and (4) (HAp-egg shell/PLGA + PRF + collagen). Cell adhesion and viability were determined using confocal microscopy and quantified using central tendency and dispersion measurements; significant differences were determined using ANOVA (p < 0.05). Results: Group 1 presented low cell viability and adhesion (3.70–10.17%); groups 2 and 3 presented high cell viability and low cell adhesion (group 2, 59.2–11.1%, group 3, 58–4.6%); group 4 presented the highest cell viability (82.8%) and moderate cell adhesion (45%) (p = 0.474). Conclusions: The effect of collagen on the HAp-egg shell/PLGA scaffold combined with PRF favored HPdLF cell adhesion and viability and could clinically have a positive effect on bone defect resolution and the regeneration of periodontal ligament tissue.

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Section: Discussionmentioning

confidence: 99%