Viability and Adhesion of Periodontal Ligament Fibroblasts on a Hydroxyapatite Scaffold Combined with Collagen, Polylactic Acid–Polyglycolic Acid Copolymer and Platelet-Rich Fibrin: A Preclinical Pilot Study

Espitia-Quiroz, Leonor C.; Fernández-Orjuela, Andrés L.; Anaya-Sampayo, Lina M.; Acosta-Gómez, Adriana P.; Sequeda-Castañeda, Luis Gonzalo; Gutiérrez, Sandra; Molina, Nelly S. Roa; Robayo, Dabeiba Adriana García

doi:10.3390/dj10090167

Cited by 6 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(105) In the construction of dental and periodontal pulp for the preservation of periodontal ligament fibroblasts (PDLF), the use of PLGA scaffold combined with PRF has shown the ability to sustain fibroblast viability. (74,127) Various signaling molecules, including growth factors and cytokines have been recognized to enhance the proliferation, migration and differentiation of dental stem cells. These molecules are naturally contained in the pulpal cells and dentin matrix, and involved in modulating dentin-pulp complex homeostasis.…”

Section: Synthetic Biomaterials Scaffoldsmentioning

confidence: 99%

Crucial Triad in Pulp-Dentin Complex Regeneration: Dental Stem Cells, Scaffolds, and Signaling Molecules

et al. 2023

View full text Add to dashboard Cite

BACKGROUND: Pulp damage can lead to dentinogenesis impairment, irreversible pulpitis, or pulp necrosis. Despite being the most used endodontic procedure to treat damaged pulp, root canal therapy only results in nonvital teeth which are prone to fractures and secondary infection. Pulp-dentin regeneration has a potential to regenerate structure similar to normal pulp-dentin complex, and can be achieved by combining dental stem cells, scaffold, and signaling molecules. This article reviews the role of various types of dental stem cells, scaffolds, signaling molecules, and their combinations in regenerating pulp-dentin complex.CONTENT: Dental pulp stem cell (DPSC), stem cell from human exfoliated deciduous teeth (SHED), and dental follicle stem cell (DFSC) were reported to regenerate pulp-dentin complex in situ. SHED might be more promising than DPSCs and DFSCs for regenerating pulp-dentin complex, since SHED have a higher proliferation potential and higher expression levels of signaling molecules. Scaffolds have characteristics resembling extracellular matrix, thus providing a suitable microenvironment for transplanted dental stem cells. To accelerate the regeneration process, exogenous signaling molecules are often delivered together with dental stem cells. Scaffolds and signaling molecules have different regenerative potential, including induction of cell proliferation and migration, formation of pulp- and/or dentin-like tissue, as well as angiogenesis and neurogenesis promotion.SUMMARY: Combinations of dental stem cells, scaffold, and signaling molecules are important to achieve the functional pulp-dentin complex formation. Current trends and future directions on regenerative endodontics should be explored. The right combination of dental stem cells, scaffold, and signaling molecules could be determined based on the patients’ characteristics. Incomplete pulp-dentin regeneration could be overcome by applying dental stem cells, scaffold, and/or signaling molecules in multiple visits.KEYWORDS: pulp-dentin regeneration, regenerative endodontics, dental stem cells, scaffold, signaling molecules

show abstract

Section: Synthetic Biomaterials Scaffoldsmentioning

confidence: 99%

Crucial Triad in Pulp-Dentin Complex Regeneration: Dental Stem Cells, Scaffolds, and Signaling Molecules

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Surprisingly, the application of nanocarriers represents a promising means to selectively deliver and concentrate drugs in ocular. Besides, well-designed nanocarriers could contribute to enhancing the ability of corneal targeting for increasing drug concentration in the cornea surface, thus improving the therapeutic efficiency of loaded drugs [21, 22]. Polylactic/polyglycolic acid (PLGA) is a biodegradable functional polymer organic compound that is randomly polymerized from two monomers – lactic acid and glycolic acid.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Efficacy of Polyglycolic Acid-Loading Tetrandrine Nanoparticles in the Treatment of Dry Eye

Li,

Tang,

et al. 2023

Ophthalmic Res

View full text Add to dashboard Cite

Introduction: Dry eye disease (DED) is a multifactor-induced disease accompanied by increased osmolarity of the tear film and inflammation of the ocular surface. Traditional anti-inflammation agent corticosteroids applied in DED treatment could result in high intraocular pressure, especially in long-term treatment. Therefore, we explored a nano drug that aimed to block the formation pathway of DED which had anti-inflammatory, sustained release, and good biocompatibility characteristics in this study. Methods: We prepared a novel nanomedicine (Tet-ATS@PLGA) by the thin film dispersion-hydration ultrasonic method and detected its nanostructure, particle size, and zeta potential. Flow cytometry was used to detect the cell survival rate of each group after 24 h of drug treatment on inflammed Statens Seruminstitut Rabbit Corneal (SIRC) cells. Observed and recorded corneal epithelial staining, tear film rupture time, and Schirmer test to detect tear secretion on the ocular surface of rabbits. The corneal epithelial thickness, morphology, and number of bulbar conjunctival goblet cells were recorded by H&E staining. Finally, we detected the expression of VEGF, IL-1β, PGE2, and TNF-α by cellular immunofluorescence staining and enzyme-linked immunosorbent assay (ELISA). Results: The encapsulation efficiency and drug loading of Tet-ATS@PLGA were 79.85% and 32.47%, respectively. At eye surface temperature, Tet can easily release from Tet-ATS@PLGA while that it was difficult to release at storage temperature and room temperature. After 2 weeks medication, Tet-ATS@PLGA can effectively improve the tear film rupture time and tear secretion time in a DED model (p <0.05). Compared with the normal group (62.34 ± 4.86 mm), the thickness of corneal epithelium in ATS (29.47 ± 3.21 mm), Tet-ATS (46.23 ± 2.87 mm), and Tet-ATS@PLGA (55.76 ± 3.95 mm) gradually increased. Furthermore, the flow cytometry indicated that Tet-ATS@PLGA can effectively promote the apoptosis of inflammatory SIRC cells, and the cellular immunofluorescence and ELISA experiments showed that the expression intensity of inflammatory factors such as VEGF, IL-1β, PGE2, and TNF-α decreased in this process. Interestingly, Tet also had the effect of reducing intraocular pressure. Conclusion: Tet-ATS@PLGA can effectively promote the apoptosis of inflammatory corneal epithelial cells, thus inhibiting the expression of inflammatory factors to block the formation of DED and improve the secretion of tear on the ocular surface.

show abstract

“…Osseous flaws that are connected to periradicular lesions can show similar phenomena. Dental implants can only be used to replace lost teeth if there is enough bone to support them sufficiently [5]. As a result, bone augmentation may substantially help implant treatment, which would otherwise not be a therapeutic option [6].…”

Section: Introductionmentioning

confidence: 99%

"Comparing and Examining the Treatment of Intraosseous Defects, Bone Grafting in Periodontal Surgery"

Nabaha

2022

ADOH

View full text Add to dashboard Cite

Treatment of periodontal osseous defects; however, the clinical benefits of this therapeutic practice require further clarification through a systematic review of randomized controlled studies. The purpose of this systematic review is to access the efficacy of bone replacement grafts in proving demonstrable clinical improvements in periodontal osseous defects compared to surgical debridement alone. Several bone graft materials have been used in the treatment of infrasonic defects. Demineralized freeze-dried bone allograft (DFDBA) has been histologically proven the material of choice for regeneration. However, platelet-rich fibrin (PRF) has been said to have several properties that aid in healing and regeneration. Hence, this study focuses on the regenerative capacity of PRF when compared with DFDBA. This study was conducted as a case study in Russia (2021Russia ( -2022 and was presented at the Japan Dental Conference 2022.

show abstract

Viability and Adhesion of Periodontal Ligament Fibroblasts on a Hydroxyapatite Scaffold Combined with Collagen, Polylactic Acid–Polyglycolic Acid Copolymer and Platelet-Rich Fibrin: A Preclinical Pilot Study

Cited by 6 publications

References 44 publications

Crucial Triad in Pulp-Dentin Complex Regeneration: Dental Stem Cells, Scaffolds, and Signaling Molecules

Crucial Triad in Pulp-Dentin Complex Regeneration: Dental Stem Cells, Scaffolds, and Signaling Molecules

Evaluating the Efficacy of Polyglycolic Acid-Loading Tetrandrine Nanoparticles in the Treatment of Dry Eye

"Comparing and Examining the Treatment of Intraosseous Defects, Bone Grafting in Periodontal Surgery"

Contact Info

Product

Resources

About