The clinical effect on plaque control of dentifrice containing dextranase

Kitamura, Tyuya; Moriguchi, Saki; Terada, Emiko; Saito, Kunio; Futakami, Katsuyuki

doi:10.5834/jdh.30.114

Cited by 4 publications

(3 citation statements)

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“…The α‐1,6‐glucoside linkage‐degrading enzyme “dextranase” and α‐1,3‐glucoside linkage‐degrading enzyme “mutanase” are promising agents against sucrose‐dependent biofilms because much of the matrix is glucan EPS (Pleszczyńska, Wiater, Janczarek, & Szczodrak, ). Dextranase in toothpaste has been proven to prevent plaque formation (Kitamura, Moriguchi, Terada, Saito, & Futakami, ). However, given the absence of glucan EPS in sucrose‐independent biofilm, we compared the degradative effect of dextranase between biofilm types and found a marked decrease in sucrose‐dependent biofilm but little change in sucrose‐independent biofilm (Figure ).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of a sucrose‐independent in vitro biofilm model of supragingival plaque

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Characterisation of a sucrose‐independent in vitro biofilm model of supragingival plaque

et al. 2017

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“…Despite considerable theoretical potential, studies carried out to evaluate the efficacy of enzymes to inhibit the formation of or reduce extant plaque have given inconclusive results. For example, whilst one early study reported significant anti-plaque activity of a dextranasecontaining dentifrice (Kitamura et al, 1980), others did not (Keyes et al, 1971;Lobene, 1971). Proteolytic formulations have also not been unequivocally successful (Odman, 1992;Robinson et al, 1975).…”

Section: Resultsmentioning

confidence: 99%

An in vitro evaluation of hydrolytic enzymes as dental plaque control agents

Ledder

Madhwani

Sreenivasan

et al. 2009

Journal of Medical Microbiology

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The plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. A quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of Actinomyces naeslundii and Streptococcus oralis (P <0.05) and of Porphyromonas gingivalis and S. oralis. Amylase significantly (P <0.05) increased the coaggregation of A. naeslundii versus Fusobacterium nucleatum and A. naeslundii versus P. gingivalis. Concomitant challenge of constant-depth film fermenter-grown plaques with the enzymes did not result in detectable ecological perturbations (assessed by differential culture and denaturing gradient gel electrophoresis). Similar dosing and analysis of multiple Sorbarod devices did not reveal increases in bacterial dispersion which could result from disaggregation of extant plaques. A short-term hydroxyapatite colonization model was therefore used to investigate possible enzyme effects on early-stage plaque development. Whilst culture did not indicate significant reductions in adhesion or plaque accumulation, a vital visual assay revealed significantly increased aggregation frequency following enzyme exposure. In summary, although hydrolytic enzymes negatively influenced binary coaggregation, they did not cause statistically significant changes in bacterial viability within plaque microcosms. In contrast, enzyme exposure increased aggregation within extant plaques.

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“…In fact, SLS was replaced in one such product by a non-ionic detergent. Such formulation difficulties might explain the variable clinical results from studies using enzyme-containing products (Etemadzadeh et al 1985), although substantial anti-plaque activity has been claimed for a toothpaste containing dextranase (Kitamura et al 1980) while a reduction in gingivitis was found with a dentifrice containing amyloglucosidase and glucose oxidase (Midda & Cooksey 1986). "Essential oils" such as thymol also have plaque-inhibiting properties but their substantivity is low and (Scheie 1989).…”

Section: Antimicrobial Agents Dentifrices and Piaque Controimentioning

confidence: 99%

Dentifrices containing new agents for the control of plaque and gingivitis: microbiological aspects

Marsh

1991

J Clinic Periodontology

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Antimicrobial agents have been proposed as playing an important role in controlling plaque and gingivitis. Unfortunately, a large number of potential compounds are unsuitable for use in dentifrices because they lack "substantivity", produce undesirable side-effects, or are incompatible with toothpaste ingredients. New agents that have been successfully incorporated into dentifrices include plant extracts, phenolic compounds and metal salts. Several products are currently being based on the phenol, Triclosan. Triclosan has a broad spectrum of antimicrobial activity against yeasts and oral bacteria. To enhance its clinical efficacy, Triclosan has been combined either with a co-polymer or with another compatible antimicrobial agent, zinc citrate. The co-polymer acts to increase the oral retention of Triclosan, and has resulted in further reductions in salivary bacterial counts in vivo. Zinc salts also have antimicrobial activity, and at low concentrations, can inhibit glycolysis and bacterial proteases. In mixed culture chemostat studies, Triclosan selectively inhibited Gram-negative periodontopathic bacteria; additive effects were obtained when zinc citrate and Triclosan were combined. In an experimental human gingivitis study, a zinc citrate/Triclosan dentifrice reduced plaque accumulation and gingivitis compared to a placebo paste; the ratio of anaerobic/aerobic bacteria and the proportions of Actinomyces species in plaque were also reduced. The prolonged use of a zinc citrate/Triclosan dentifrice neither significantly altered the ecology of supragingival plaque nor led to the selection of Triclosan-resistant bacteria. The data suggest that dentifrices containing new antimicrobial agents could be of clinical relevance in the prevention and control of plaque and gingivitis.

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The clinical effect on plaque control of dentifrice containing dextranase

Cited by 4 publications

References 10 publications

Characterisation of a sucrose‐independent in vitro biofilm model of supragingival plaque

Characterisation of a sucrose‐independent in vitro biofilm model of supragingival plaque

An in vitro evaluation of hydrolytic enzymes as dental plaque control agents

Dentifrices containing new agents for the control of plaque and gingivitis: microbiological aspects

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