Characterisation of a sucrose‐independent <i>in vitro</i> biofilm model of supragingival plaque

Tsutsumi, Kimiko; Maruyama, Masato; Uchiyama, Akira; Shibasaki, K

doi:10.1111/odi.12779

Cited by 14 publications

(26 citation statements)

References 37 publications

(41 reference statements)

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“…The prevailing ecological plaque hypothesis [44,45] describes an ecological shift (collapse) towards the enrichment of acidogenic and acid-tolerant species in low pH environments, such as after sugar consumption [13,16,46]. The present findings are consistent with this hypothesis, as several such species were found to be enriched in two of the three cluster groups with the highest sucrose intake.…”

Section: Discussionsupporting

confidence: 89%

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Oral Microbiota Profile Associates with Sugar Intake and Taste Preference Genes

et al. 2020

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Oral microbiota ecology is influenced by environmental and host conditions, but few studies have evaluated associations between untargeted measures of the entire oral microbiome and potentially relevant environmental and host factors. This study aimed to identify salivary microbiota cluster groups using hierarchical cluster analyses (Wards method) based on 16S rRNA gene amplicon sequencing, and identify lifestyle and host factors which were associated with these groups. Group members (n = 175) were distinctly separated by microbiota profiles and differed in reported sucrose intake and allelic variation in the taste-preference-associated genes TAS1R1 (rs731024) and GNAT3 (rs2074673). Groups with higher sucrose intake were either characterized by a wide panel of species or phylotypes with fewer aciduric species, or by a narrower profile that included documented aciduric- and caries-associated species. The inferred functional profiles of the latter type were dominated by metabolic pathways associated with the carbohydrate metabolism with enrichment of glycosidase functions. In conclusion, this study supported in vivo associations between sugar intake and oral microbiota ecology, but it also found evidence for a variable microbiota response to sugar, highlighting the importance of modifying host factors and microbes beyond the commonly targeted acidogenic and acid-tolerant species. The results should be confirmed under controlled settings with comprehensive phenotypic and genotypic data.

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Section: Discussionsupporting

confidence: 89%

“…This shift mirrors the tooth biofilm dysbiosis in dental caries [14]. Sucrose exposure in vivo and assessments of targeted species support these experimental findings [15][16][17]. However, associations between sugar intake and untargeted characterization of the oral microbiota remain sparsely studied [4].…”

Section: Introductionmentioning

confidence: 79%

Oral Microbiota Profile Associates with Sugar Intake and Taste Preference Genes

et al. 2020

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“…Recently, interactions of fusobacteria with streptococci have been described; however, the genes involved in the formation of biofilms of different species have not been identified in most of them [19][20][21][22][23][24][25]. This study aimed to identify the proteins associated with the adhesion and coaggregation of F. nucleatum and S. gordonii.…”

Section: Introductionmentioning

confidence: 99%

Identification of Proteins Associated with the Formation of Oral Biofilms

Gómez

Amaranto

Torres

et al. 2021

Pesqui. Bras. Odontopediatria Clín. Integr.

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Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.

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“…The physical attachment between the interacting species is mediated by speci c cellular adhesion proteins in their outer membranes [15]. For example, proteins of the SrtA family that anchor to the cell wall have been identi ed, such as SspA/SspB of S. gordonii, which allow interactions with other streptococci and other oral species, including Porphyromonas gingivalis, Actinomyces, and Candida [16][17][18][19]. Egland et al [20] showed that the SspA/SspB homologue of S. mutans binds to the host's saliva proteins; the host's matrix proteins, including type I collagen, bronectin, laminin, and keratin; and serum components such as brinogen.…”

Section: Introductionmentioning

confidence: 99%

Identification and localization of proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms

Gómez¹,

Bacilio-Amaranto²,

Torres³

et al. 2020

Preprint

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Background:To successfully colonize the oral cavity, bacteria must adhere directly or indirectly to the oral surfaces available. Fusobacterium nucleatum plays an important role in the development of the oral biofilm community due to its broad adhesion capabilities, serving as a bridge between the members of the oral biofilm community that cannot be directly joined together. The purpose of this study was to identify and localize the proteins associated with the formation of biofilms of Streptococcus gordonii and F. nucleatum. Methods: Multispecies biofilms were identified by amplification of the srtA and radD genes by real-time PCR. Biofilm cells cultured with sucrose were counted. The protein concentrations in the membrane and cytoplasmic fractions were quantified by western blot. Results: The proteins HSP40 and GAPDH were detected in the cytoplasmic fraction of biofilm and F. nucleatum, respectively. The available anti-GAPDH antibody is specific for GAPDH produced by F. nucleatum, which indicated the coaggregation of F. nucleatum on S. gordonii. Conclusions: HSP40 was only detected in the cytoplasmic fraction of the biofilms, making it one of the essential proteins for adherence. This complex set of interactions could have critical implications for the formation and maturation of oral biofilms in vivo and could provide clues to the mechanism behind the distribution of organisms within the human oral cavity.

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Characterisation of a sucrose‐independent in vitro biofilm model of supragingival plaque

Abstract: Our findings suggest that sucrose-independent biofilm may have cariogenicity as with sucrose-dependent biofilm. These in vitro models can help further elucidate plaque-induced caries aetiology and develop new anticaries agents.

Cited by 14 publications

References 37 publications

Oral Microbiota Profile Associates with Sugar Intake and Taste Preference Genes

Oral Microbiota Profile Associates with Sugar Intake and Taste Preference Genes

Identification of Proteins Associated with the Formation of Oral Biofilms

Identification and localization of proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms

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