The chromosomal localization, expression pattern and polymorphism analysis of porcine FSCN1 gene differently expressed from LongSAGE library

Tang, Zhonglin; Zhang, Xingju; Yang, S. L.; Mu, Yulian; Cui, Wentao; Hong, Ai; Li, K.

doi:10.1007/s11033-009-9742-9

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…The previous studies showed that the establishment of the total number of fibers involves two major waves of fiber generation: primary muscle fiber formation at 35-60 dpc and secondary muscle fibers assembled at 54-90 dpc 37-39, the expression patterns of OLFML3 gene during prenatal skeletal muscle development in our study indicating that it mainly involved in the formation of primary muscle fibers. The differential expression patterns from Landrace to Tongcheng pigs also showed that there are developmental synchronizations in prenatal skeletal muscle development between the two pig breeds, the result was consistent with our previous report 31, 40, which concluded Landrace pigs may have a quicker muscle growth rate than Tongcheng pigs. Prenatal muscle development programs postnatal performance.…”

Section: Discussionsupporting

confidence: 91%

OLFML3 Expression is Decreased during Prenatal Muscle Development and Regulated by MicroRNA-155 in Pigs

Zhao¹,

Zhang²,

Hou³

et al. 2012

Int. J. Biol. Sci.

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The Olfactomedin-like 3 (OLFML3) gene has matrix-related function involved in embryonic development. MicroRNA-155 (miR-155), 21- to 23-nucleotides (nt) noncoding RNA, regulated myogenesis by target mRNA. Our LongSAGE analysis suggested that OLFML3 gene was differently expressed during muscle development in pig. In this study, we cloned the porcine OLFML3 gene and detected its tissues distribution in adult Tongcheng pigs and dynamical expression in developmental skeletal muscle (12 prenatal and 10 postnatal stages) from Landrace (lean-type) and Tongcheng (obese-type) pigs. Subsequently, we analyzed the interaction between OLFML3 and miR-155. The OLFML3 was abundantly expressed in liver and pancreas, moderately in lung, small intestine and placenta, and weakly in other tissues and postnatal muscle. There were different dynamical expression patterns between Landrace and Tongcheng pigs during prenatal skeletal muscle development. The OLFML3 was down-regulated (33-50 days post coitus, dpc), subsequently up-regulated (50-70 dpc), and then down-regulated (70-100 dpc) in Landrace pigs, while in Tongcheng pigs, it was down-regulated (33-50 dpc), subsequently up-regulated (50-55 dpc) and then down-regulated (55-100 dpc). There was higher expression in Tongcheng than Landrace in prenatal muscle from 33 to 60 dpc, and opposite situation from 65 to 100 dpc. Dual luciferase assay and real time PCR documented that OLFML3 expression was regulated by miR-155 at mRNA level. Our research indicated that OLFML3 gene may affect prenatal skeletal muscle development and was regulated by miR-155. These finding will help understanding biological function and expression regulation of OLFML3 gene in mammal animals.

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Section: Discussionsupporting

confidence: 91%

OLFML3 Expression is Decreased during Prenatal Muscle Development and Regulated by MicroRNA-155 in Pigs

Zhao¹,

Zhang²,

Hou³

et al. 2012

Int. J. Biol. Sci.

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“…FSCN1 is an actinbinding protein critical to cell adhesion, cell motility, and cellcell interactions. In normal prenatal pig skeletal muscle, FSCN1 expression increases during major muscle developmental spurts, but postnatally it is expressed highly only in brain tissues during accelerated neural cell development [133] . The CREB pathway is often activated in different metastatic human cancers, causing the upregulation of FSCN1 expression [134] .…”

Section: Cell Factors Deregulated In Cancer By Myomirsmentioning

confidence: 99%

Roles of the canonical myomiRs miR-1, -133 and -206 in cell development and disease

Mitchelson¹

2015

WJBC

136

132

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MicroRNAs are small non-coding RNAs that participate in different biological processes, providing subtle combinational regulation of cellular pathways, often by regulating components of signalling pathways. Aberrant expression of miRNAs is an important factor in the development and progression of disease. The canonical myomiRs (miR-1, -133 and -206) are central to the development and health of mammalian skeletal and cardiac muscles, but new findings show they have regulatory roles in the development of other mammalian non-muscle tissues, including nerve, brain structures, adipose and some specialised immunological cells. Moreover, the deregulation of myomiR expression is associated with a variety of different cancers, where typically they have tumor suppressor functions, although examples of an oncogenic role illustrate their diverse function in different cell environments. This review examines the involvement of the related myomiRs at the crossroads between cell development/ tissue regeneration/tissue inflammation responses, and cancer development. Core tip: The roles of the canonical muscle-associated microRNAs are reviewed, including microRNA families miR-1 and miR-133, and single miR-206, which are collectively known as the "myomiRs". The myomiRs act at the crossroads of the molecular regulation of muscle cells, linking between pathways for cell differentiation, development and maintenance, but also potentiate aberrant cell growth in numerous non-muscle cancers. Typically myomiRs are downregulated in cancers, but some myomiRs are upregulated in a few cancers, yet each dysregulation event advances tumor progression. The review examines normal and disease-linked molecular changes associated with the myomiRs, and collates the extensive literature into accessible tables. REVIEW

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“…Fascin-1 is abundant early in development, especially in the central nervous system (CNS) and migrating cells, and is then downregulated as cells mature (De Arcangelis et al, 2004; Zhang et al, 2008; Zanet et al, 2009; Tang et al, 2010). In this paper, ‘fascin’ refers to the product of the Fascin-1 genes ( FSCN1 in humans, MIM#602689; Fscn1 in mouse; and singed in Drosophila , FBgn0003447).…”

Section: Introductionmentioning

confidence: 99%

A cell-based fascin bioassay identifies compounds with potential anti-metastasis or cognition-enhancing functions

Kraft

Kahn

Medina-Franco

et al. 2012

Disease Models &Amp; Mechanisms

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SUMMARYThe actin-bundling protein fascin is a key mediator of tumor invasion and metastasis and its activity drives filopodia formation, cell-shape changes and cell migration. Small-molecule inhibitors of fascin block tumor metastasis in animal models. Conversely, fascin deficiency might underlie the pathogenesis of some developmental brain disorders. To identify fascin-pathway modulators we devised a cell-based assay for fascin function and used it in a bidirectional drug screen. The screen utilized cultured fascin-deficient mutant Drosophila neurons, whose neurite arbors manifest the ‘filagree’ phenotype. Taking a repurposing approach, we screened a library of 1040 known compounds, many of them FDA-approved drugs, for filagree modifiers. Based on scaffold distribution, molecular-fingerprint similarities, and chemical-space distribution, this library has high structural diversity, supporting its utility as a screening tool. We identified 34 fascin-pathway blockers (with potential anti-metastasis activity) and 48 fascin-pathway enhancers (with potential cognitive-enhancer activity). The structural diversity of the active compounds suggests multiple molecular targets. Comparisons of active and inactive compounds provided preliminary structure-activity relationship information. The screen also revealed diverse neurotoxic effects of other drugs, notably the ‘beads-on-a-string’ defect, which is induced solely by statins. Statin-induced neurotoxicity is enhanced by fascin deficiency. In summary, we provide evidence that primary neuron culture using a genetic model organism can be valuable for early-stage drug discovery and developmental neurotoxicity testing. Furthermore, we propose that, given an appropriate assay for target-pathway function, bidirectional screening for brain-development disorders and invasive cancers represents an efficient, multipurpose strategy for drug discovery.

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The chromosomal localization, expression pattern and polymorphism analysis of porcine FSCN1 gene differently expressed from LongSAGE library

Cited by 8 publications

References 23 publications

OLFML3 Expression is Decreased during Prenatal Muscle Development and Regulated by MicroRNA-155 in Pigs

OLFML3 Expression is Decreased during Prenatal Muscle Development and Regulated by MicroRNA-155 in Pigs

Roles of the canonical myomiRs miR-1, -133 and -206 in cell development and disease

A cell-based fascin bioassay identifies compounds with potential anti-metastasis or cognition-enhancing functions

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