The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes

Choi, Kyoung-Hwan; Choi, Byung Hyune; Park, Sora; Kim, Byoung Ju; Min, Byoung Hyun

doi:10.1016/j.biomaterials.2010.03.053

Cited by 116 publications

(84 citation statements)

References 50 publications

(50 reference statements)

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“…heart valves, blood vessels, skin and cartilage [8,[28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]) scaffolds. In the case of articular cartilage, numerous studies have demonstrated that scaffolds derived from devitalized cartilage are chondroinductive and show great promise for regenerating damaged joints [8,[29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells

et al. 2014

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Section: Introductionmentioning

confidence: 99%

Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells

et al. 2014

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“…Previous studies have shown that CD-ECM may serve as a biomaterial scaffold. The CD-ECM scaffold supports chondrogenic differentiation of MSCs and helps maintain the phenotype of these cells in vivo (21,22). This phenomenon is responsible for the major composition of CD-ECM involved in collagen type II and HA.…”

Section: Discussionmentioning

confidence: 99%

“…The composition of the CD-ECM scaffold consists of: 73% collagen type I, 16% GAGs, and 11% water (20 (21,22). The aims of the present study were to determine the wound-healing effects of CD-ECM on corneal injury and to elucidate the cellular mechanism underlying these effects.…”

Section: Introductionmentioning

confidence: 99%

Effects of topical chondrocyte-derived extracellular matrix treatment on corneal wound healing, following an alkali burn injury

Yang

Lee

et al. 2014

Molecular Medicine Reports

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Abstract. Numerous treatments have been used in the management of corneal chemical burns; however, no optimal treatment for corneal chemical burns currently exists. The present study investigated the effects of topical chondrocyte-derived extracellular matrix (CD-ECM) treatment on corneal wound healing, using an alkali burn mouse model. Topical treatment with CD-ECM was shown to reduce corneal opacity following an alkali burn. A histological examination observed the presence of regenerated epithelial cells and a small number of inflammatory cells in the corneas of CD-ECM-treated mice. The majority of the inflammatory cells present in the corneas of the phosphate-buffered saline (PBS)-treated mice were neutrophils that expressed matrix metalloproteinase (MMP)-9. The amount of neutrophils was significantly reduced in the corneas of the CD-ECM-treated mice. Furthermore, the expression levels of interleukin (IL)-8 were significantly reduced in the CD-ECM treatment group, but not in the mice that received the PBS treatment. The results of the present study indicate that CD-ECM treatment may accelerate wound healing in a model of alkali burn-induced corneal injury. The therapeutic mechanism may be associated with accelerated reepithelialization and reduced recruitment of MMP-9-expressing neutrophils, through inhibiting the production of IL-8.

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“…Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. [23][24][25][26][27][28]. One study compared an ECM scaffold derived from porcine chondrocytes with a polyglycolic acid scaffold, demonstrating that MSC chondrogenesis was more rapid and the chondrogenic phenotype was maintained longer with the ECM scaffold [23].…”

Section: Introductionmentioning

confidence: 99%

“…[23][24][25][26][27][28]. One study compared an ECM scaffold derived from porcine chondrocytes with a polyglycolic acid scaffold, demonstrating that MSC chondrogenesis was more rapid and the chondrogenic phenotype was maintained longer with the ECM scaffold [23]. In addition, chondrogenesis of MSCs was successful using 3D collagen microspheres produced by decellularization of porcine chondrocytes [24] and an acellular electrospun poly (ε-caprolactone)/ECM composite scaffold [27].…”

Section: Introductionmentioning

confidence: 99%

Effect of chondrocyte-derived early extracellular matrix on chondrogenesis of placenta-derived mesenchymal stem cells

et al. 2015

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The extracellular matrix (ECM) surrounding cells contains a variety of proteins that provide structural support and regulate cellular functions. Previous studies have shown that decellularized ECM isolated from tissues or cultured cells can be used to improve cell differentiation in tissue engineering applications. In this study we evaluated the effect of decellularized chondrocyte-derived ECM (CDECM) on the chondrogenesis of human placenta-derived mesenchymal stem cells (hPDMSCs) in a pellet culture system. After incubation with or without chondrocyte-derived ECM in chondrogenic medium for 1 or 3 weeks, the sizes and wet masses of the cell pellets were compared with untreated controls (hPDMSCs incubated in chondrogenic medium without chondrocyte-derived ECM). In addition, histologic analysis of the cell pellets (Safranin O and collagen type II staining) and quantitative reverse transcription-PCR analysis of chondrogenic markers (aggrecan, collagen type II, and SOX9) were carried out. Our results showed that the sizes and masses of hPDMSC pellets incubated with chondrocyte-derived ECM were significantly higher than those of untreated controls. Differentiation of hPDMSCs (both with and without chondrocyte-derived ECM) was confirmed by Safranin O and collagen type II staining. Chondrogenic marker expression and glycosaminoglycan (GAG) levels were significantly higher in hPDMSC pellets incubated with chondrocyte-derived ECM compared with untreated controls, especially in cells precultured with chondrocyte-derived ECM for 7 d. Taken together, these results demonstrate that chondrocyte-derived ECM enhances the chondrogenesis of hPDMSCs, and this effect is further increased by preculture with chondrocyte-derived ECM. This preculture method for hPDMSC chondrogenesis represents a promising approach for cartilage tissue engineering.

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The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes

Cited by 116 publications

References 50 publications

Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells

Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells

Effects of topical chondrocyte-derived extracellular matrix treatment on corneal wound healing, following an alkali burn injury

Effect of chondrocyte-derived early extracellular matrix on chondrogenesis of placenta-derived mesenchymal stem cells

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