The Chinese medicine Kuan-Sin-Yin improves liver function in patients with chronic hepatitis C: A randomised and placebo-controlled trial

Liu, Chia-Yu; Ko, Pin Hao; Yen, Hung-Rong; Cheng, Chen Hung; Li, Yu Hsien; Liao, Zih Han; Hsu, Cheng Hsiung

doi:10.1016/j.ctim.2016.06.004

Cited by 12 publications

(16 citation statements)

References 48 publications

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“…Dried roots of C. pilosula and A. membranaceus were used as crude drugs, as reported in our previous papers [14–16]. The roots were soaked in water at a ratio of 1:3 (g:mL) and boiled at 100 °C for 4 h. The decoctions were dehydrated using a freeze dryer (Panchum FD DC-3000, Panchum, Kaohsiung, Taiwan), filtered using a 0.22-µm filter (Merck Millipore, Tullagreen, Carrigtwohill, IRL), and re-dissolved in ddH 2 O.…”

Section: Methodsmentioning

confidence: 99%

“…The details of the preparation steps and high-performance liquid chromatography (HPLC) equipment and results have been described previously [14]. Because the same herbs were used in the present study, we only showed the following key settings: The powdered roots of both plants (0.2 g) were suspended in 70% methanol and filtered through a 0.2-μm HPLC membrane.…”

Section: Methodsmentioning

confidence: 99%

“…The internal transcribed spacer (ITS) analysis protocols have been described previously but only ITS2 sequencing results have been published [14, 15]. The ITS1 for all herbs were sequenced using the following primers: (forward) 5′-GGAAGTAAAAGTCGTAACAAGG-3′; (reverse) 5′-TCCTCCTCCGCTTATTGATATGC-3′ (Table 1).…”

Section: Methodsmentioning

confidence: 99%

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Identifying the functions and biomarkers of Codonopsis pilosula and Astragalus membranaceus aqueous extracts in hepatic cells

Huang

Chang

et al. 2019

Chin Med

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BackgroundHomeostasis is a crucial concept used to describe the condition of patients and the roles of herbs in traditional Chinese medicine. Qi-deficiency pattern is one of the conditions when loss of homeostasis and is usually characterized by symptoms including lassitude, spontaneous sweating, and a weak pulse, which are not easy to quantitate. Codonopsis pilosula and Astragalus membranaceus were usually prescribed for carriers with hepatitis and patients with metastatic colon cancer, because these patients tended to experience fatigue. However, crude drugs were prescribed based on the exterior symptoms of patients without controlling clinical setting, such as gender, age, and dietary habits. Limited molecular evidence of using gene expression as the guide for description is available. Therefore, the purpose of this study was to identify potential and objective biomarkers of these two qi-related drugs in a simplified cellular system.MethodsAqueous extracts of crude qi-tonifying herbs, C. pilosula and A. membranaceus, and that of a qi-consuming drug, Citrus reticulata, were prepared. Human liver cancer HepG2 cells were treated with the extracts of qi-tonifying herbs for 24 h. Differentially expressed genes were identified using microarrays and quantitative RT-PCR (qRT-PCR) and validated in two other hepatocellular cell lines, Huh7 and L-02.ResultsA total of 67 differentially expressed probes that responded to both herbs were identified. A pathway analysis revealed that these genes were involved in the development, growth, movement, and viability of the liver cells.ConclusionsAfter qRT-PCR validation and examination of clinical data from public domains, our results showed that two genes, GDF15 and HMOX1, could serve as biomarkers in liver cells for identifying responses after treatment with C. pilosula and A. membranaceus.Electronic supplementary materialThe online version of this article (10.1186/s13020-019-0233-1) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Identifying the functions and biomarkers of Codonopsis pilosula and Astragalus membranaceus aqueous extracts in hepatic cells

Huang

Chang

et al. 2019

Chin Med

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“…The results showed that 6 weeks of treatment with KSY significantly reduced HCV viral load, and ALT and AST levels were obviously decreased. 72 Although KSY is not effective enough to gain SVR, it shows effective hepatoprotection for CHC patients. However, the long-term effects of KSY still remain to be evaluated in the future.…”

Section: Kuan Sin Yin (Ksy)mentioning

confidence: 99%

Herbal Medicines for Hepatitis C Virus Infection: The Exploratory Journey from Bench to Bedside Still Has a Long Way to Go

Xiao-ya¹,

Zhang²,

Xie³

et al. 2019

Journal of Exploratory Research in Pharmacology

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Hepatitis C virus (HCV) infects at least 150 million people chronically worldwide. It is a major risk factor for cirrhosis, hepatocellular carcinoma, and death. Direct-acting antiviral therapy is very efficacious in treating HCV infection but it is inaccessible and unavailable in some developing countries. Therefore, searching for more effective and easily accessible regimens remains an urgent need. The aim of this article is to review the anti-HCV effects of herbal medicines from experimental to clinical evidence, and discuss current issues, hurdles and future perspectives for their application from bench to bedside. Numerous in vitro studies have indicated that many herbs work effectively in exerting anti-HCV activities. Yet, only a few animal experiments have been conducted that demonstrate the anti-HCV effects of these medicines; in addition, these results do not show an ability to eliminate the virus completely from the infected animals. Thus far, clinical trials have produced inconclusive anti-HCV results in terms of efficacy and safety, presumably due to the lack of the quality of methodologies used in the trials. In conclusion, despite apparent anti-HCV activities in vitro, clinical efficacy and safety of herbal medicines for the treatment of HCV infection have not been revealed convincingly. More animal studies using ideal models and more well-designed clinical trials with a larger sample sizes and longer treatment periods, taking the body habitus into consideration, are required to further assess the efficacy and safety of herbal medicines for HCV infection.

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“… 6 Modern pharmacological and clinical studies have demonstrated that TCM induces autophagic cell death, prevents resistance to anticancer drugs, and relieves severe side effects during conventional anticancer treatments. 7 , 8 …”

Section: Introductionmentioning

confidence: 99%

Current development in integrative therapy of traditional Chinese medicine for cancer treatment: A mini-review

Tai

2020

Journal of Traditional and Complementary Medicine

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Cancer is a major public health problem worldwide, and there has been a sustained rise in its incidence in both developing and developed countries. Although there are currently numerous effective therapeutic options for cancer, they sometimes exhibit resistance and obvious side effects. Traditional Chinese medicine (TCM) currently plays a major role in cancer therapy by downregulating the growth of cancer cells through various pathways and by relieving side effects. Studies in cultured human malignant cell lines have demonstrated that Solanum nigrum can control cancer cell proliferation and cancer progression by inducing autophagic and apoptotic cell death. Case–control studies have indicated that TCM can relieve the side effects of cancer therapy. This review provides brief insights into the anticancer effects of TCM, the side effects relieved by TCM, and the role of TCM doctors in cancer treatment.

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The Chinese medicine Kuan-Sin-Yin improves liver function in patients with chronic hepatitis C: A randomised and placebo-controlled trial

Cited by 12 publications

References 48 publications

Identifying the functions and biomarkers of Codonopsis pilosula and Astragalus membranaceus aqueous extracts in hepatic cells

Identifying the functions and biomarkers of Codonopsis pilosula and Astragalus membranaceus aqueous extracts in hepatic cells

Herbal Medicines for Hepatitis C Virus Infection: The Exploratory Journey from Bench to Bedside Still Has a Long Way to Go

Current development in integrative therapy of traditional Chinese medicine for cancer treatment: A mini-review

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