The Chicken-Embryo-Lethal-Orphan (CELO) Virus as a Tissue-Culture Contaminant

Yates, Vance J.; Ablashi, Dharam V.; Chang, P. W.; Fry, D. E.

doi:10.2307/1587916

Cited by 11 publications

(4 citation statements)

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“…These signs were reduced if avian encephalomyelitis was present (Yates and Fry, 1957). However, chicks hatched from eggs inoculated with the same strain did not show nervous signs (Yates et al, 1962). …”

Section: Association With Nervous Diseasementioning

confidence: 99%

Avian adenoviruses ‐ a review

1977

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Section: Association With Nervous Diseasementioning

confidence: 99%

Avian adenoviruses ‐ a review

1977

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“…Three-times plaque-purified type 1 AvAV (CELO strain) was propagated in chicken embryo kidney (CEK) cells prepared from specific-pathogen-free (SPF) embryonated eggs (SPAFAS, Norwich, Conn.) in Hanks' or Earles' modified basal medium (Eagle) supplemented with 7.5 or 2.0% fetal bovine serum without antibiotics [9]. Stocks were maintained free of AvA-AV contamination.…”

Section: Virus Propagation and Purificationmentioning

confidence: 99%

Populations of Infectious Virus Produced during Avian Adenovirus-Associated Virus Infection

1980

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Multiple rounds of infection in vitro or in vivo with avian adenovirus-associated virus (AvA-AV) and avian adenovirus (AvAV) result in production of both heavy (H) and light (L) infectious forms. In this study, the infectious AvA-AV progeny produced at different hours during recombined dual infection of cells with either H or L AvA-AV and AvAV was determined by equilibrium CsCl centrifugation and infectivity assay. In both types of infection, H virions were found early, both intra- and extracellularly, whereas L virions were found late. The data indicate an H to L particle density shift during infection. Virus-specified cell-dependent factors mediated the process extracellularly, as activity was detected in infected cell-conditioned medium and in lysates of infected cells but not in medium or components of uninfected cells. The shift in density was accompanied by a conversion in particle type. H and L virions differed in size and conformation as evidenced by differences in serological cross-reactivity and physical-chemical stability during heat inactivation, ultrasonic disruption and DNA extraction.

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“…chicken-embryo-lethal-orphan (CELO) virus exists as a latent virus in eggs and produces subclinical infections in chickens (Yates and Fry, 1957;Yates et al, 1962). When inoculated intracranially in wet-chicks that do not carry CELO antibody, neurological symptoms become evident within 12 days and the chicks eventually succumb (Yates, 1960).…”

Section: Thementioning

confidence: 99%

Morphology of Tumours Induced in Hamsters by CELO Virus, Tumour Tissue, and Tumour Cells Grown in Culture

Mancini¹,

Jasty²,

Anderson³

et al. 1972

Br J Cancer

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Summary.-Tumours in hamsters, induced by the chicken-embryo-lethal-orphan (CELO) virus, by tumour tissue transplants, or by tumour cells grown in culture, were well circumscribed solid tumours and covered by a thin capsule -like structure. All were fibrosarcomata. However, tumours produced by the 3 inocula exhibited the following histological differences. Neoplasms induced by CELO virus were generally less differentiated and were composed of cells with polygonal or oval nuclei and indistinct cytoplasmic boundaries. Numerous multinucleated bizarre giant cells were found. Those produced by tumour tissue transplants were more differentiated and were composed of spindle shaped cells with abundant collagen fibre formation. Neoplasms induced by tumour cells grown in culture were generally undifferentiated with many mitotic figures and contained numerous giant cells.Cells from tumours induced by CELO virus or tumour transplants produced similar morphologies when cultured in vitro. The cell cultures consisted of large cells with oval or rounded large nuclei and prominent nucleoli. Multinucleated giant cells, cells in mitosis, and a disorganized growth pattern were also characteristic of the cell cultures. However, mitosis and a piling-up of cells occurred more frequently with cell cultures derived from the CELO virus-induced tumour.

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The Chicken-Embryo-Lethal-Orphan (CELO) Virus as a Tissue-Culture Contaminant

Cited by 11 publications

References 0 publications

Avian adenoviruses ‐ a review

Avian adenoviruses ‐ a review

Populations of Infectious Virus Produced during Avian Adenovirus-Associated Virus Infection

Morphology of Tumours Induced in Hamsters by CELO Virus, Tumour Tissue, and Tumour Cells Grown in Culture

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