The chemotactic effect of a dermal papilla cell-derived factor on outer root sheath cells

Fujie, Takeshi; Katoh, Shoji; Oura, Hajimu; Urano, Yoshio; Arase, Seiji

doi:10.1016/s0923-1811(00)00130-4

Cited by 43 publications

(37 citation statements)

References 27 publications

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“…22,27,28 We also speculate that paracrine factors from hDPCs may help induce the proliferation of hair matrix cells due to RGE. However, we did not observe an increase in the expression of IGF-1, HGF, or VEGF, which are well-known mitogenic factors secreted by dermal papillae.…”

Section: Figmentioning

confidence: 88%

Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

Park

Cho

et al. 2015

Journal of Medicinal Food

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Ginseng has been shown to promote hair growth in several recent studies. However, its effects on human hair follicles and its mechanisms of action have not been sufficiently elucidated. This study aimed to investigate the hair growthpromoting effects of red ginseng extract (RGE) and its ginsenosides. The proliferative activities of cultured human hair follicles treated with RGE and ginsenoside-Rb1 were assessed using Ki-67 immunostaining. Their effects on isolated human dermal papilla cells (hDPCs) were evaluated using cytotoxicity assays, immunoblot analysis of signaling proteins, and the determination of associated growth factors. We examined the ability of RGE and ginsenosides to protect hair matrix keratinocyte proliferation against dihydrotestosterone (DHT)-induced suppression and their effects on the expression of androgen receptor. The in vivo hair growth-promoting effect of RGE was also investigated in C57BL/6 mice. Both RGE and ginsenoside-Rb1 enhanced the proliferation of hair matrix keratinocytes. hDPCs treated with RGE or ginsenoside-Rb1 exhibited substantial cell proliferation and the associated phosphorylation of ERK and AKT. Moreover, RGE, ginsenoside-Rb1, and ginsenoside-Rg3 abrogated the DHT-induced suppression of hair matrix keratinocyte proliferation and the DHT-induced upregulation of the mRNA expression of androgen receptor in hDPCs. Murine experiments revealed that the subcutaneous injection of 3% RGE resulted in more rapid hair growth than the negative control. In conclusion, RGE and its ginsenosides may enhance hDPC proliferation, activate ERK and AKT signaling pathways in hDPCs, upregulate hair matrix keratinocyte proliferation, and inhibit the DHT-induced androgen receptor transcription. These results suggest that red ginseng may promote hair growth in humans.

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Section: Figmentioning

confidence: 88%

Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

Park

Cho

et al. 2015

Journal of Medicinal Food

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“…Many growth factors have been reported as derived from DPCs [25,[41][42][43]. Some of these factors might contribute to the higher number of epidermis layers and the stem cell population present in the DPC composite skin constructs in vitro.…”

Section: Discussionmentioning

confidence: 99%

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Leirós

Kusinsky

Drago³

et al. 2014

Stem Cells Translational Medicine

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Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs). In vitro, the presence of DPCs induced a more regular and multilayered stratified epidermis with more basal p63-positive cells and invaginations. The DPC-containing constructs more accurately mimicked the skin architecture by properly stratifying the differentiating HFSCs and developing a well-ordered epithelia that contributed to more closely recapitulate an artificial human skin. This acellular dermal matrix previously repopulated in vitro with HFSCs and DFs or DPCs as the dermal component was grafted in nude mice. The presence of DPCs in the composite substitute not only favored early neovascularization, good assimilation and remodeling after grafting but also contributed to the neovascular network maturation, which might reduce the inflammation process, resulting in a better healing process, with less scarring and wound contraction. Interestingly, only DPC-containing constructs showed embryonic hair bud-like structures with cells of human origin, presence of precursor epithelial cells, and expression of a hair differentiation marker. Although preliminary, these findings have demonstrated the importance of the presence of DPCs for proper skin repair.

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“…To promote hair growth and initiate anagen, it is essential that expression of factors maintaining anagen is increased, such as IGF-1, basic fibroblast growth factor (bFGF), KGF, and VEGF, while decreasing expression of cytokines promote apoptosis, such as TGF-β, and IL-1. 32,33) VEGF plays a central role in promoting angiogenesis as well as influencing diverse cell functions including cell survival, proliferation, and generation of nitric oxide and prostacyclin.…”

Section: Discussionmentioning

confidence: 99%

<i>In Vivo</i> Hair Growth-Promoting Effect of Rice Bran Extract Prepared by Supercritical Carbon Dioxide Fluid

Choi

Jeon²,

Moon³

et al. 2014

Biological & Pharmaceutical Bulletin

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The potential hair growth-promoting activity of rice bran supercritical CO 2 extract (RB-SCE) and major components of RB-SCE, linoleic acid, policosanol, γ-oryzanol, and γ-tocotrienol, were evaluated with the histological morphology and mRNA expression levels of cell growth factors using real-time reverse transcriptase-polymerase chain reaction (PCR) in C57BL/6 mice. RB-SCE showed hair growth-promoting potential to a similar extent as 3% minoxidil, showing that the hair follicles were induced to be in the anagen stage. The numbers of the hair follicles were significantly increased. In addition, mRNA expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and keratinocyte growth factor (KGF) were also significantly increased and that of transforming growth factor-β (TGF-β) decreased in RB-SCE-treated groups. Among the major components of RB-SCE, linoleic acid and γ-oryzanol induced the formation of hair follicles according to examination of histological morphology and mRNA expression levels of cell growth factors. In conclusion, our results demonstrate that RB-SCE, particularly linoleic acid and γ-oryzanol, promotes hair growth and suggests RB-SCE can be applied as hair loss treatment. Key words rice bran supercritical CO 2 extract; hair growth-promoting activity; in vivoAlthough hair loss is not a mortal disorder, it has a great impact on a person's self-respect, mental health, and overall quality of life. Approximately 50-60 hairs are normally lost per day, which does not have a noticeable effect on appearance; however, excess loss (>100) results in baldness. Androgenetic alopecia is the most common type of hair loss, affecting millions of both men and women.1) There are many causes of hair loss in men and women, including diseases, nutritional deficiency, aging, hormone imbalance, and stress. Androgenetic alopecia may occur as early as the teenage years, but typically begin in the later decades of life. 2,3) Hair loss affects at least half of all men by the age of 50 and up to 70% of 70-year-old men. 4)

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The chemotactic effect of a dermal papilla cell-derived factor on outer root sheath cells

Cited by 43 publications

References 27 publications

Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

<i>In Vivo</i> Hair Growth-Promoting Effect of Rice Bran Extract Prepared by Supercritical Carbon Dioxide Fluid

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