The Chemistry of the Acid-Fast Reaction

Lartigue, Donald J.; Fite, George L.

doi:10.1177/10.5.611

Cited by 7 publications

(2 citation statements)

References 3 publications

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“…It has been reported that ether-hydrochloric acid removes an acid-fast staining lipid fraction from Mycobacterium (22). Lartigue and Fite (13), however, state that exhaustively defatted tubercle bacilli remain acid-fast.…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition of Chlamydospores of Candida albicans

Jansons

Nickerson

1970

J Bacteriol

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A variety of analytical techniques was employed to study the composition of the chlamydospore of Candida albicans. The outer, thin, electron-transparent layer was found to be composed of glucan, together with a small amount of chitin. The inner, thick, electron-dense layer is proteinaceous. The central structure is composed largely of ribonucleic acid and lipid globules. In addition to being acid-fast, the chlamydospore was found to contain glycolipids and to lose the property of acidfastness on extraction with ethanol-ether. Skujins et al. (21). Streptomyces species no. 3 (kindly supplied by M. Alexander, Cornell University) was maintained on a medium consisting of a 0.5% suspension of distiller's solubles (Brown, Forman Co., Louisville, Ky.) in distilled water, adjusted to pH 7.6, and solidified with 3% agar (Difco). For enzyme production, the organism was grown in a basal salts liquid medium (21) to which autoclaved, lyophilized cells of C. albicans were added (5 g/liter). This medium (25 liters) was sterilized in a 40-liter fermentor 922 on July 31, 2020 by guest

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Section: Resultsmentioning

confidence: 99%

Chemical Composition of Chlamydospores of Candida albicans

Jansons

Nickerson

1970

J Bacteriol

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“…Pouco tempo depois Lubinoff (1888 a, b) afirmava ter aperfeiçoado o método, substituindo o ácido fênico por ácido bórico. Posteriormente, vários pesquisadores estudaram e deram novas justificativais para o uso do fenol, entre as quais aumentar a solubilidade da fucsina nos lipídeos da parede bacilar (Hanks, 1961b;Lartigue, 1962;Harada, 1976b;. Com o uso da fucsina e a introdução do álcool, o método passou a ser conhecido como Ziehl-Neelsen, segundo relata Hallberg (l946).…”

Section: Fómula Estruturalunclassified

Comportamento tintorial do Mycobacterium leprae: revisão histórica

1983

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GASMoC method: a phenol-free technique to detect acid-fast bacilli

Gomes

Amaral

Santos

et al. 2019

Histochem Cell Biol

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The genre Mycobacterium includes a series of pathological species, such as M. tuberculosis, which is important for pathology laboratories to detect for a correct diagnosis. The Ziehl-Neelsen technique (ZNT) is the most commonly histochemical method used to detect these bacilli and uses a heated mixture of carbol-fuchsine, which contains basic fuchsine and phenol. Whereas the former component is responsible for the pinkish staining of acid-fast mycobacteria, the role of phenol is not completely understood and it has been suggested that its exclusion does not impact the detection ability of the ZNT. Since phenol is highly toxic and induces several injuries, the goal of this study is to determine the detection capacity of mycobacteria through a method that excludes the use of phenol. Accordingly, the GASMoC method, a modified ZNT that employs a solution of aqueous basic fuchsine heated at 37 °C, was tested on histological samples positive for mycobacteria and the results were compared to that of the ZNT. Data demonstrated that the GASMoC method was able to detect acid-fast bacilli (AFB) in all analyzed cases. Remarkably, microscopic inspection of mycobacteria was easier when the GASMoC method was applied. In conclusion, our study demonstrates that AFB detection does not require phenol and that the GASMoC method, a phenol-free technique, may substitute the ZNT in pathology laboratories.

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The Chemistry of the Acid-Fast Reaction

Cited by 7 publications

References 3 publications

Chemical Composition of Chlamydospores of Candida albicans

Chemical Composition of Chlamydospores of Candida albicans

Comportamento tintorial do Mycobacterium leprae: revisão histórica

GASMoC method: a phenol-free technique to detect acid-fast bacilli

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