A variety of analytical techniques was employed to study the composition of the chlamydospore of Candida albicans. The outer, thin, electron-transparent layer was found to be composed of glucan, together with a small amount of chitin. The inner, thick, electron-dense layer is proteinaceous. The central structure is composed largely of ribonucleic acid and lipid globules. In addition to being acid-fast, the chlamydospore was found to contain glycolipids and to lose the property of acidfastness on extraction with ethanol-ether. Skujins et al. (21). Streptomyces species no. 3 (kindly supplied by M. Alexander, Cornell University) was maintained on a medium consisting of a 0.5% suspension of distiller's solubles (Brown, Forman Co., Louisville, Ky.) in distilled water, adjusted to pH 7.6, and solidified with 3% agar (Difco). For enzyme production, the organism was grown in a basal salts liquid medium (21) to which autoclaved, lyophilized cells of C. albicans were added (5 g/liter). This medium (25 liters) was sterilized in a 40-liter fermentor 922 on July 31, 2020 by guest