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Mucor rouxii may follow either one of two patterns of morphogenesis (mold-yeast dimorphism), depending on the atmosphere of incubation. Under air or N2, a filamentous (moldlike) growth

Mucor rouxii was grown under three different atmospheres of incubation: air, N2, and C02 in parallel cultures. The atmosphere of incubation markedly affected nutritional requirements, growth, and morphogenesis. Absence of MATERIALS AND METHODS M. rouxii strain IM 80, of the culture collection of the Institute of Microbiology, and M. subtilissimus NRRL 1909, obtained through the courtesy of C. W. Hesseltine, Northern Utilization Research and Development Division, Peoria, Ill., were employed. A defined medium was prepared with the following ingredients dissolved in distilled water to make 1 liter: glucose, 20 g; KH2PO4, 3.0 g; MgS04.7H20, 0.5 g; ZnSO4.7H20, 1.8 mg; FeSO4-7H20, 1.0 mg; MnS04-H20, 0.3 mg; CuS04.5H20, 0.4 mg; thiamine, 1 mg; and nicotinic acid, 1 mg; pH was adjusted to 4.5 with 841

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