The Chemistry of Glomerular Basement Membrane and Its Relation to Collagen^*

Abstract

The basement membrane from glomeruli of normal dogs was isolated by a procedure involving sonication and differential centrifugation. The amino acid composition of the isolated material in several

Cytochemical Studies of Ameloblasts and the Surface Layer of Enamel of the Rat Incisor at the Maturation Stage

“…The biochemical analyses of the basal lamina revealed that it partially consisted of collagenous components (MIZRA and BERMAN, 1966;KEFALIDES and WINZLER, 1966;KEFALIDES, 1967;DANIELS and CHU, 1975;CARLSON et al, 1978). KAHL and PEARSON (1967) and EVAN et al (1976), in fact, succeeded in digesting the basal lamina with collagenase.…”

Detection of Anti-Glomercular Basement Membrane Antibodies by a Radioimmunological Technique CLINICAL APPLICATION IN HUMAN NEPHROPATHIES

“…In four of these "positive" patients, IgG fractions were isolated from serum and were shown to contain a significant binding activity. DISCUSSION It is well known (24,25) that GBM are mostly glycoproteins containing two different oligosaccharidic chains; one is a disaccharide formed of equimolecular amounts of glucose and galactose, while the other is a heteropolysaccharide composed of 'mannose, fucose, galactose, sialic acid, and hexosamines (13,14). However, the basement membrane structure responsible for the production of anti-GBM antibodies remains a matter of controversy.…”

Study on the Porcine Tubular Basement Membrane

“…The renal glomerular basement membrane (GBM) and tubular basement membrane (TBM) have been shown to be glycoprotein containing approximately 10% of carbohydrates (Kefalides and Winzler 1966;Spiro 1967a; Mahieu and Winand 1970;Westberg and Michael 1973;Ferwerda et al 1974a, b;Sato et al 1975a, b;Ferwerda and van Dijek 1975). As reviewed by Spiro (1972), GBM appeared to play a vital physiological role by acting as a selective filter, while TBM may play an important role in the highly selective reabsorption function of the tubule.…”

“…It may therefore show a more precise figure of the chemical changes under the pathological conditions. Although GBM has been studied extensively (Kefalides and Winzler 1966;Spiro 1967a;Mahieu and Winand 1970;Westberg and Michael 1973), there has been few reports concerning TBM (Mahieu and Winand 1970;Ferwerda et al 1947a, b;Spiro 1972). Mahieu and Winand (1970) obtained TBM from human kidney.…”