The chemical composition of regenerating mouse liver

Tsuboi, Kenneth K.; Yokoyama, Hisako O.; Stowell, Robert E.; Wilson, Margaret E.

doi:10.1016/0003-9861(54)90342-2

Cited by 36 publications

(16 citation statements)

References 14 publications

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“…Thus the results ot Price and Laird (5) show increases on a cellular basis in most of the liver cytoplasmic fractions within the first 24 hours after hepatectomy. However, in contradistinction to uterine tissue the activity of the succinoxidase system in regenerating liver increases less rapidly than the total protein (4,12) although there is an increase in total cellular activity in both organs (12).…”

Section: Discussionmentioning

confidence: 99%

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Effect of Estradiol Injection on the Cytoplasmic Fractions of the Myometrium in the Ovariectomized Rat

Wakid

Needham

1961

The Journal of Cell Biology

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In earlier work (14, 15) some aspects of the constitution and enzymic activities of cell fractions from rat myometrium were studied at the end of pregnancy and after ovariectomy. In the present work a study is made of the effect on these same properties of estradiol injection into ovariectomized rats. MATERIALS AND METHODSUnless otherwise stated all materials and methods were as described previously (14,15). Experiments consisted in studying with respect to time the effects of a single dose of estradiol on the myofilament and nucleus fraction (N), the mitochondrial fraction (M), the microsomal fraction (P), and the soluble fraction (S) of the ovariectomized rat myometrium. The separation between these fractions, which was effected by differential centrifugation, is not clear cut, there being unavoidable overlapping. Thus fraction N contains some unbroken cells, and although fraction M is essentially devoid of myofilaments it is contaminated with collagen fibres and microsomal material. Fraction P is also contaminated with mitochondrial material as evidenced by its relatively high succinic dehydrogenase activity in the ovariectomized state.Estradiol monobenzoate (supplied in 1 mg. ampoules by the British Drug Houses, Ltd.) was diluted with arachis oil immediately before use. Each rat was injected subcutaneously with 10 #g. of estradiol contained in 0.5 ml. of oil. Depending on the material available, batches of 6 to 12 uteri were pooled for each experiment. All uteri were slit open with fine scissors, and the endometrium was removed by scraping with a scalpel.Succinic dehydrogenase was assayed colourimetritally with KaFe(CN)6 as electron acceptor and was determined only in fractions M and P, the activity in fi-actions N and S being negligible. ATPasO activity was determined by measuring the amount of inorganic phosphate liberated after 6 minutes of incubation with 5 mM ATP and 5 mM MgSO4.In the extraction of phospholipids the waterethanol mixture was omitted for fear of dissolving low molecular weight RNA. Lipids were therefore separated with two 95 per cent ethanol extractions in the 1Abbreviations: ATPase, adenosinetriphosphatase; DNA, desoxyribonucleic acid; RNA, ribonucleic acid.cold followed by one ethanol-chloroform (3:1) and one ether extraction at room temperature. Following extraction of the phospholipids from the various fractions, the RNA and DNA were determined by phosphorus estimation after alkaline hydrolysis according to the Schmidt-Thannhauser and Schneider technique.Total nitrogen was determined by the Kjeldahl method.All values for the ovariectomized and pregnant states were compiled from data in our previous work (15). Table I shows a slight increase in the DNA P content of the uterus starting 18 hours after injection which is in agreement with earlier microscopic observations that some cell division does occur under the influence of estradiol (9). On the assumption that the DNA content of the cell is constant (11, 13) all other values are given per rag. DNA P in order to express the changes pe...

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Section: Discussionmentioning

confidence: 99%

“…In fraction N some part of the RNA increase probably occurs in the nuclei, as in regenerating liver (12). In the latter Jardetsky et al (1) report that the highest rate of p~2 incorporation is in the nuclear RNA.…”

Section: Ribonucleic Acids and Phospholipidsmentioning

confidence: 95%

Effect of Estradiol Injection on the Cytoplasmic Fractions of the Myometrium in the Ovariectomized Rat

Wakid

Needham

1961

The Journal of Cell Biology

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“…The cytology and biochemistry of liver regenerating after partial hepatectomy has been studied extensively (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13), and much information is available concerning the behavior of the various cell constituents during regeneration. Among the investigators of regenerating liver, there seems to be universal agreement concerning an early accumulation of fat following partial hepatectomy.…”

Section: Introductionmentioning

confidence: 99%

A Fine Structure Study of Lipid in Mouse Liver Regenerating After Partial Hepatectomy

Trotter

1964

The Journal of Cell Biology

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The fine structure of liver 3½ to 72 hours after partial hepatectomy has been compared with that of liver from sham-operated animals; all animals were 60- to 90-day old male mice of the C3H strain. Numerous small bodies with diameters ranging from 300 to 1,000 A have been observed distributed randomly throughout the cytoplasm of the hepatic parenchymal cells at early intervals after partial hepatectomy. In material fixed in osmium tetroxide and embedded in methacrylate, they appear as uniformly electron-opaque bodies, but in permanganate-fixed liver, they display only a peripheral rim of electron-opaque material surrounding a clear core. Each of these cytoplasmic bodies appears to be located within a vesicle. A few of the opaque bodies are also present in sinusoids and in the spaces of Disse; these bodies are not located within vesicular structures. Fat droplets of various sizes are easily distinguished in regenerating liver; with the increase in number of these fat droplets at later postoperative intervals, there occurs a concomitant decrease in the number of cytoplasmic bodies. It is suggested that the cytoplasmic bodies contain some lipid component. Possible explanations of the origin, nature, and fate of the cytoplasmic bodies are discussed.

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“…The liver in hypertrophy after partial hepatectomy has been the organ of choice for these studies, the most comprehensive being those of Price & Laird (1950) and Tsuboi, Yokoyama, Stowell & Wilson (1954). Some investigations have also been made on the kidney in compensatory hypertrophy after unilateral nephrectomy (Mandel, Mandel & Jacob, 1950;Vegni, 1953;Dianzani & Biaggini, 1954).…”

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confidence: 99%