The Chemical Composition and Serological Reactions of Lipopolysaccharides from Serogroups A, B, X, and Y <i>Neisseria meningitidis</i>

Jennings, Harold J.; Hawes, Gordon; Adams, G. A.; Kenny, C. P.

doi:10.1139/o73-178

Cited by 41 publications

(23 citation statements)

References 9 publications

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“…Although the presence of sialic acid in meningococcal glycolipids has been reported by numerous investigators (19,23,27,28,41), it should be noted that the results of these and other studies (10,20,42,45,46,49,51,58) were derived from material cleaved under acidic conditions that could degrade possible constituents of LOS (e.g., sialic acid, fucose, and phosphate) (Fig. 6B).…”

Section: Resultsmentioning

confidence: 54%

Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis

et al. 1991

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Monoclonal antibodies (MAb) 3F11 and 06B4 recognize epitopes that are conserved on gonococcal lipooligosaccharides (LOS), present on some meningococcal LOS, and conserved on human erythrocytes. LOS of some group B and C prototype meningococcal LOS strains (LOS serotypes Ll to L8) treated with neuraminidase showed increased expression of the 3F11 and 06B4 MAb-defined epitopes. Neuraminidasetreated LOS separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver stained showed a shift in migration from a component with a mass of approximately 4.8 kDa to a component with a mass of between 4.5 and 4.6 kDa. The same strains grown in medium with excess CMP-N-acetylneuraminic acid had LOS that shifted in migration to a slightly higher component (mass, approximately 4.8 kDa). Chemical analysis of the neuraminidase-digested products from one LOS indicated it contained approximately 1.5% sialic acid. Covalent linkage between sialic acid and the LOS was confirmed by analysis of de-O-acylated and dephosphorylated LOS by liquid secondary ion mass spectrometry. These studies show that some meningococci contain sialic acid in their LOS, that the sialic acid is cleaved and lost in conventional acetic acid hydrolysis, and that the sialic acid alters the expression of MAb-defined epitopes. Recent studies have shown that when gonococci are grown in the presence of CMP-N-acetylneuraminic acid (CMP-NANA), sialic acid is incorporated into the LOS component of 4.5 kDa that binds MAbs 3F11 and 06B4 (36, 44). The sialylated component no longer binds the MAbs (4, 36). Treatment of the LOS with neuraminidase restores these epitopes, both on organisms grown in vitro (36) and on organisms in vivo in urethral exudates (4). Although the 3F11 and 06B4 epitopes are of similar specificity and are both expressed on gonococcal LOS (37), the 06B4 epitope is expressed much better than is the 3F11 epitope on meningococcal LOS of group B and C strains (30, 35 Bacterial strains. The prototype group B and C meningococcal LOS strains (38, 62) have been described previously. The surface glycolipids (lipooligosaccharides [LOS]) of

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Section: Resultsmentioning

confidence: 54%

Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis

et al. 1991

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“…Lipopolysaccharide (LPS) was visualised as the typical "smeared" appearance distal to this band. All meningococcal LPS, except that of group A strains, has been shown to consist of the same tetrasaccharide backbone bound to 2-keto-3-deoxyoctonate (KDO) (Jennings et al, 1973), making immunological cross-reactivity possible at this site. The structure of LPS of commensal Neisseria spp.…”

Section: Discussionmentioning

confidence: 99%

Detection of antibodies to common antigens of pathogenic and commensal Neisseria species

Cann

Rogers

1989

Journal of Medical Microbiology

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Summary. Sera from 29 children and six adults were used to investigate the nature of antigenic cross-reactivity between Neisseria polysaccharea, N . lactamica and N . meningitidis B, 15P1.16 by immunoblotting. Major common antigens of 68-70 Kda, 60-65 Kda and 15-20 Kda were detected. Antibody directed against them uniformly decreased after absorption of the sera with the three different Neisseria species. Antigens of 55 Kda and 35 Kda specific to N . meningitidis, and one of 43 Kda specific to N . lactamica, were also demonstrated. Antibody against all antigens was more prevalent in bactericidal than in non-bactericidal sera, although these differences were statistically not significant. Differences in antibody prevalence between carriers of Neisseria spp. and non-carriers of these organisms were even less marked. Examination of sera by whole-cell enzyme-linked immunosorbent assay against N . meningitidis B, 15P 1.16 and N . lactamica gave an absorbance ratio of 1 : 1. Only four sera from children showed no reactivity against the meningococcal strain. These common antigens are likely to be important in vaccine development.

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“…Preparation of lipid A from LPS. Lipid A was prepared as described by Jennings et al (4). About 60 mg of LPS was heated at 1000C with 5 ml of 0.1 N acetic acid for 2 h. The lipid precipitate was removed by centrifugation and washed with water, dissolved in chloroform, and reprecipitated in acetone (1 part lipid to 3 parts acetone).…”

Section: Methodsmentioning

confidence: 99%

“…Colonial types identified by Kellogg et al (8), of importance since it was shown that type 1 and 2 gonococci are virulent for humans whereas types 3 and 4 are not, are currently being investigated in regard to immunological and chemical differences. Perry et al (15) examined the LPS of type 1 and 4 gonococci and found that type 1 "smooth" preparations possessed characteristic sugars which were absent or different in concentration from those in "rough" type 4 LPS. Common to both were lipid A and a core fragment composed of n-glucose, Dgalactose, heptose, and 2-keto-3-deoxyoctulosonic acid (KDO).…”

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confidence: 99%

Composition of the lipopolysaccharide of Neisseria gonorrhoeae

Wiseman¹,

Caird²

1977

Infect Immun

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Analysis of glycose and fatty acid content of lipopolysaccharide extracted from 38 strains of Neisseria gonorrhoeae indicated that glycoses common to colonial types 1 to 5 were glucose, mannose, and galactose. N-acetylneuraminic acid, 2keto-3-deoxyoctulosonic acid (KDO), glucosamine, and galactosamine were also invariably present. Virulent colonial types 1 and 2 contained no rhamnose, in contrast to avirulent types 3 to 5 and several strains ofthe nonpathogenic species N. sicca and N. lactamica. Fucose, characteristic of these nonpathogenic species, was not present in the gonococci. Variation in the concentration of individual glycoses in different strains was also noted. Mannose-KDO, galactose-KDO, and glucose-KDO ratios of virulent gonococci exceeded those of avirulent organisms, except that the correlation for glucose was not quite so striking. This relationship was not found in N. sicca and N. lactamica strains. Fatty acid analyses of lipid A from gonococci showed that 10-, 12-, 14-, 16-, and 18-carbon acids, as well as 3-hydroxytetradecanoic acid, were present, but differences in concentration between colonial types, although evident in some cases, appeared less significant than glycose content.

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The Chemical Composition and Serological Reactions of Lipopolysaccharides from Serogroups A, B, X, and Y Neisseria meningitidis

Cited by 41 publications

References 9 publications

Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis

Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis

Detection of antibodies to common antigens of pathogenic and commensal Neisseria species

Composition of the lipopolysaccharide of Neisseria gonorrhoeae

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