Composition of the lipopolysaccharide of Neisseria gonorrhoeae

Abstract

Analysis of glycose and fatty acid content of lipopolysaccharide extracted from 38 strains of Neisseria gonorrhoeae indicated that glycoses common to colonial types 1 to 5 were glucose, mannose, and galactose. N-acetylneuraminic acid, 2keto-3-deoxyoctulosonic acid (KDO), glucosamine, and galactosamine were also invariably present. Virulent colonial types 1 and 2 contained no rhamnose, in contrast to avirulent types 3 to 5 and several strains ofthe nonpathogenic species N. sicca and N. lactamica. Fucose, characteristic of these nonpathogenic species, was not present in the gonococci. Variation in the concentration of individual glycoses in different strains was also noted. Mannose-KDO, galactose-KDO, and glucose-KDO ratios of virulent gonococci exceeded those of avirulent organisms, except that the correlation for glucose was not quite so striking. This relationship was not found in N. sicca and N. lactamica strains. Fatty acid analyses of lipid A from gonococci showed that 10-, 12-, 14-, 16-, and 18-carbon acids, as well as 3-hydroxytetradecanoic acid, were present, but differences in concentration between colonial types, although evident in some cases, appeared less significant than glycose content.

Effects of human neutrophil granule extracts on macromolecular synthesis in Neisseria gonorrhoeae

“…Mo-xted to, grame drzakowski et al found numerous bactericidal ted to, gram-fractions from chromatographically fractionated ainca previous granule contents and went on to more comien amous pletely describe a fraction (valley AB from a cient amounts Sephadex G-100 column) that contained a po-T isedkibitegd tent bactericidal cationic protein with an appar-P5 M) present ent molecular weight of approximately 36,500 (6, ion by CCP of 7) Gram-negative bacteria, possessing lipopolyhio cOPacofi sacchande with the Ra chemotype, appeared to cherechla osli have similar sensitivities to Modrzakowski's valthereas loss of ley AB protein and Weiss's B/PI. The gonococci ilrty in gono-used in this report apparently have the Ra chemcurs only after d decrease in otype lipopolysaccharide (12,24) and appear to (iv) The bac-react to partially purified B/PI protein with the ted by heating same sensitivity as Ra mutants of E. coli (mangonococcicidal uscript in preparation). -0.179 (0.05) 0.580 (0.02) a At 0 min, the spectrophotometer was zeroed at 550 nm to the PBS control containing gonococci and was used as the blank for the other assays.…”

Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae

“…It has been shown that T1 variants may contain S type lipopolysaccharide (21), in addition to R type lipopolysaccharide (22,28), the only type found in T4 variants. Furthermore, T1 variants have a larger amount of acidic glycoses, N-acetylneuraminic acid and 2-ketodeoxyoctulosonic acid than T4 variants (30).…”

Lipooligosaccharides (LOS) of Neisseria gonorrhoeae and Neisseria meningitidis have components that are immunochemically similar to precursors of human blood group antigens. Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human erythrocytes.

“…minal tetrasaccharide (lacto-N-neotetraose, LNnT) of paragloboside (nLc4Cer), a glycosphingolipid precursor ofthe major human blood group antigens (10), and terminates in the disaccharide N-acetyllactosamine (LacNAc) . Although complete chemical structures have not been reported for the oligosaccharides of any gonococcal LOS, several laboratories have reported that the LOS of various strains were composed ofdifferent ratios ofthe same monosaccharides (Gal, Glc, G1cNAc, Hep, dOclA) as meningococcal LOSS (11)(12)(13)(14)(15)(16) .…”