The Charcot–Leyden crystal protein galectin‐10 is not a major determinant of human regulatory T‐cell function

Arroyo-Hornero, Rebeca; Aegerter, Helena; Hamad, Ibrahim; Côrte-Real, Beatriz F.; Staes, Katrien; Woning, Bas van der; Verstraete, Kenneth; Savvides, Savvas N.; Lambrecht, Bart; Kleinewietfeld, Markus

doi:10.1111/all.15332

Cited by 6 publications

(9 citation statements)

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“…In patients with atopic dermatitis, an elevation in galectin-10 expression is found in interleukin (IL)-22-producing T cells [ 39 ]. Although CD25+ regulatory T cells were shown to express galectin-10 [ 40 ], a recent report showed no significant galectin-10 mRNA and protein expression using multiple experimental modalities [ 41 ]. Galectin-10 and CLCs are also found in basophils [ 42 , 43 ], whereas the presence of CLCs in clinical samples is almost consistently associated with eosinophilic diseases [ 44 ].…”

Section: Abundance and Distribution Of Galectin-10 In Human Eosinophilsmentioning

confidence: 99%

“…Galectin-10 in CD25+ regulatory T cells contributes to their proliferative capacity and suppressive effects [ 40 ]. However, a recent rebuttal report indicated that the number of galectin-10 regulatory T cells was low, and galectin-10 had no effect on regulatory T cell viability, proliferation, or expression of their master transcription factor FOXP3 [ 41 ].…”

Section: Biological Functions Of Clcs and Galectin-10mentioning

confidence: 99%

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Galectin-10 as a Potential Biomarker for Eosinophilic Diseases

et al. 2022

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Galectin-10 is a member of the lectin family and one of the most abundant cytoplasmic proteins in human eosinophils. Except for some myeloid leukemia cells, basophils, and minor T cell populations, galectin-10 is exclusively present in eosinophils in the human body. Galectin-10 forms Charcot–Leyden crystals, which are observed in various eosinophilic diseases. Accumulating studies have indicated that galectin-10 acts as a new biomarker for disease activity, diagnosis, and treatment effectiveness in asthma, eosinophilic esophagitis, rhinitis, sinusitis, atopic dermatitis, and eosinophilic granulomatosis with polyangiitis. The extracellular release of galectin-10 is not mediated through conventional secretory processes (piecemeal degranulation or exocytosis), but rather by extracellular trap cell death (ETosis), which is an active cell death program. Eosinophils undergoing ETosis rapidly disintegrate their plasma membranes to release the majority of galectin-10. Therefore, elevated galectin-10 levels in serum and tissue suggest a high degree of eosinophil ETosis. To date, several studies have shown that galectin-10/Charcot–Leyden crystals are more than just markers for eosinophilic inflammation, but play functional roles in immunity. In this review, we focus on the close relationship between eosinophils and galectin-10, highlighting this protein as a potential new biomarker in eosinophilic diseases.

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Section: Abundance and Distribution Of Galectin-10 In Human Eosinophilsmentioning

confidence: 99%

Section: Biological Functions Of Clcs and Galectin-10mentioning

confidence: 99%

Galectin-10 as a Potential Biomarker for Eosinophilic Diseases

et al. 2022

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“…Histone epigenetic marks and chromHMM data from the Roadmap Epigenomics Project (26) were downloaded from WashU EpiGenome Browser (v52.1.0) (27). Data were extracted from human CD4 + CD25 -Th and CD4 + CD25 + CD127 -Treg primary cells isolated from blood of healthy donors (donors 62 and 332) as described before (28).…”

Section: Epigenetic Datamentioning

confidence: 99%

“…Reads were aligned to the hg19 genome reference using STAR (2.5.0e) and a maximum of five mismatches were allowed. Gene counts were retrieved using htseq-count using the "union" option as described before (28).…”

Section: Reanalysis Of Published Transcriptomic Datasetsmentioning

confidence: 99%

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Dissecting the role of CSF2RB expression in human regulatory T cells

et al. 2022

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Colony stimulating factor 2 receptor subunit beta (CSF2RB; CD131) is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. Interestingly, FOXP3+ regulatory T cells (Tregs), which play a pivotal role in prevention of autoimmunity have been demonstrated to highly overexpress CSF2RB and genome-wide association studies (GWAS) identified CSF2RB as being linked to autoimmune diseases like multiple sclerosis (MS). However, the exact biological role of CD131 in human Tregs has not been defined yet. Here we investigated CD131 importance on Treg phenotype and function in a broad range of in vitro studies. Although we could not recognize a specific function of CSF2RB; CD131 in human Tregs, our data show that CD131 expression is vastly restricted to Tregs even under stimulatory conditions, indicating that CD131 could aid as a potential marker to identify Treg subpopulations from pools of activated CD4+ T cells. Importantly, our analysis further demonstrate the overexpression of CSF2RB in Tregs of patients with autoimmune diseases like MS and systemic lupus erythematosus (SLE) in comparison to healthy controls, thereby indicating that CSF2RB expression in Tregs could serve as a potential novel biomarker for disease.

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