The characterization, selenylation and antidiabetic activity of mycelial polysaccharides from Catathelasma ventricosum

Liu, Yuntao; You, Yuxian; Li, Yiwen; Zhang, Lan; Yin, Leilei; Shen, Yingbin; Cheng, Li; Chen, Hong; Chen, Shujuan; Hu, Bin; Chen, Shuai

doi:10.1016/j.carbpol.2017.06.050

Cited by 66 publications

(27 citation statements)

References 36 publications

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“…The solution was placed at room temperature for 24 h, neutralized to pH 6.0-7.0 with 50% acetic acid, dialyzed as described above, and concentrated to a volume of 10 mL followed by freeze-drying. Subsequent treatments were performed as described in previous study [34].…”

Section: Periodate Oxidation−smith Degradation 25 Mg Of Jp1mentioning

confidence: 99%

“…Method. We determined the tertiary structure of JP1 using Congo Red Method as previous study with some modifications [34]. 1 mg/L solution of JP1 polysaccharide was mixed with 0.1 mmol/L Congo Red solution and then 1 mol/L NaOH solution was added to make the final concentration of 0.05, 0.1, 0.15, 0.20, 0.30, 0.35, and 0.40 mol/L.…”

Section: Determination Of Tertiary Structure Using Congo Redmentioning

confidence: 99%

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Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells

Chen

Peng

et al. 2017

International Journal of Polymer Science

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Polysaccharide is one of the important active ingredients of Cantharellus cibarius. The aims of this work were to analyze preliminary characterization and to investigate immunostimulating activity of a novel water-soluble neutral polysaccharide named JP1, which was purified from the fruiting body of Cantharellus cibarius using DEAE-FF chromatography and Sephadex G-100 chromatography. The characteristics of JP1 were determined by HPGPC, FT-IR spectra, gas chromatography, and Congo Red Method. Immunostimulating activity of JP1 was investigated in RAW264.7 cells. Results indicated that JP1 consisted of L-Arabinose, D-Mannose, D-Glucose, and D-Galactose in a molar ratio of 1 : 1.06 : 1.95 : 1.17 with a molecular weight of 336 kDa. JP1 is nontoxic to RAW264.7 cells at this concentration range (62.5-1000 g/mL). Furthermore, JP1 can promote mouse peritoneal macrophages to secrete NO and enhance the secretion of macrophages' cytokines IL-6 in RAW264.7 cells. These results suggested that JP1 could have potential immunostimulating activity applications as medicine or functional food.

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Section: Periodate Oxidation−smith Degradation 25 Mg Of Jp1mentioning

confidence: 99%

Section: Determination Of Tertiary Structure Using Congo Redmentioning

confidence: 99%

Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells

Chen

Peng

et al. 2017

International Journal of Polymer Science

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“…In the case of SPs, the molecular weight can be determined with high-performance gel permeation (or gel filtration) chromatography [ 149 , 156 , 227 , 228 , 229 ], and the presence and quantification of Se can be confirmed by ICP-OES, ICP-MC, or AAS [ 149 , 182 , 229 ]. More in-depth characterization can be obtained with Fourier-transform infrared spectroscopy (FTIR), UV spectroscopy, and AFM [ 156 ].…”

Section: Analytical Methods For Selenium Detection and Speciationmentioning

confidence: 99%

Selenium Analysis and Speciation in Dietary Supplements Based on Next-Generation Selenium Ingredients

Constantinescu-Aruxandei

Frîncu

Capră

et al. 2018

Nutrients

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Selenium is essential for humans and the deficit of Se requires supplementation. In addition to traditional forms such as Se salts, amino acids, or selenium-enriched yeast supplements, next-generation selenium supplements, with lower risk for excess supplementation, are emerging. These are based on selenium forms with lower toxicity, higher bioavailability, and controlled release, such as zerovalent selenium nanoparticles (SeNPs) and selenized polysaccharides (SPs). This article aims to focus on the existing analytical systems for the next-generation Se dietary supplement, providing, at the same time, an overview of the analytical methods available for the traditional forms. The next-generation dietary supplements are evaluated in comparison with the conventional/traditional ones, as well as the analysis and speciation methods that are suitable to reveal which Se forms and species are present in a dietary supplement. Knowledge gaps and further research potential in this field are highlighted. The review indicates that the methods of analysis of next-generation selenium supplements should include a step related to chemical species separation. Such a step would allow a proper characterization of the selenium forms/species, including molecular mass/dimension, and substantiates the marketing claims related to the main advantages of these new selenium ingredients.

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“…The molecular weight (Mw) distributions were measured using high performance gel permeation chromatography (HPGPC) with an Agilent 1100 HPLC system equipped with a Waters 2410 refractive index detector and a TSK-GEL G5000 PWxL column (7.8 mm × 300 mm, TOSOH Co., Japan) as previously described [20]. The mobile phase consisted of ultrapure water which owed at a rate of 0.8 mL/min and a temperature of 30 °C.…”

Section: Molecular Weight Analysismentioning

confidence: 99%

Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity

Fan

Chen

et al. 2020

Preprint

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BackgroundThis study aimed to verify whether lentinan (LNT) administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs. MethodsTwenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15.ResultsDietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, whose molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05). ConclusionThose results suggest that LNT administration increases the piglets’ resistance to RV-induced stress, likely by supporting intestinal immunity.

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The characterization, selenylation and antidiabetic activity of mycelial polysaccharides from Catathelasma ventricosum

Cited by 66 publications

References 36 publications

Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells

Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells

Selenium Analysis and Speciation in Dietary Supplements Based on Next-Generation Selenium Ingredients

Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity

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