The Characterization of Clinically Important Gram Negative Anaerobic Bacilli by Conventional Bacteriological Tests

Duerden, B. I.; Holbrook, W. P.; Collee, J. G.; Watt, B.

doi:10.1111/j.1365-2672.1976.tb04164.x

Cited by 74 publications

(39 citation statements)

References 28 publications

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“…Most strains can be identified by a relatively simple set of conventional bacteriological tests adapted to their specific requirements. The identification scheme used in these studies (Duerden et al 1976 and in the Press) allowed prompt and accurate identification of most of the Bacteroides spp. encountered.…”

Section: Discussionmentioning

confidence: 99%

“…isolated from clinical specimens in the routine diagnostic laboratory were identified by a combined set of tolerance tests, antibiotic disk resistance tests, morphological and biochemical tests. This investigation was part of the evaluation of a scheme for the identification of clinically important gram-negative anaerobic bacilli by conventional bacteriological tests (Duerden et al 1976; in the Press).…”

Section: Introductionmentioning

confidence: 99%

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The identification of gram-negative anaerobic bacilli isolated from clinical infections

Duerden

1980

J. Hyg.

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SUMMARYGram-negative anaerobic bacilli isolated from specimens submitted to the routine diagnostic bacteriology laboratory and regarded as significant pathogens were identified by conventional bacteriological tests; 399 strains isolated from 356 specimens submitted from 332 patients were studied and most were readily identified by the results of a combined set of morphological, biochemical, tolerance and antibiotic disk resistance tests. B. fragilis has particular pathogenic potential and was the commonest species isolated, accounting for > 50 % of strains. The next commonest was B. asaccharolyticus with 55 strains, and 16 other species or groups were represented by smaller numbers. Many (68 %) were from infections related to the gastro-intestinal tract, but there were significant numbers from infections of the male and female genito-urinary tracts, the head, neck and central nervous system and from a variety of soft tissue infections. Most infections were mixed, and a pure culture of a Bacteroides sp. was obtained from only 26 % of infections; two or more strains of Bacteroides were recovered from 55 infections. The specific identification of Bacteroides may help the bacteriologist to judge the significance of laboratory findings, influence the patient's management and prognosis and help determine the source of infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The identification of gram-negative anaerobic bacilli isolated from clinical infections

Duerden

1980

J. Hyg.

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“…Disk resistance tests (Duerden et al, 1976(Duerden et al, , 1980 were performed with metronidazole (5 pug), neomycin ( 1000 pg), kanamycin (1 000 pg), penicillin (2 units) and rifampicin (15 pg) disks on blood agar; the inoculum was taken directly from a 48-h culture on blood agar. Plates were examined after anaerobic incubation for 48 h; strains with zones of inhibition < 15 mm diameter were recorded as resistant in terms of this test.…”

Section: Antibiotic Susceptibility Testsmentioning

confidence: 99%

“…Strains were tested for production of indole and lipase, hydrolysis of aesculin, digestion of charcoal-gelatin disks, and production of acid from glucose, lactose, sucrose, rhamnose, cellibiose, salicin, galactose and xylan by the methods of Duerden et al (1976Duerden et al ( , 1980 as modified for rapid tests by Rotimi et al (1980). Oxidase test.…”

Section: Biochemical Testsmentioning

confidence: 99%

A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Duerden

Eley

Goodwin

et al. 1989

Journal of Medical Microbiology

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Summary. Clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as Bacteroides ureolyticus) from superficial ulcers and soft tissue infections (1 9, non-gonococcal, non-chlamydia1 urethritis (1 2) and adult periodontal disease (14) were compared with reference strains of B. ureolyticus, B. gracilis and Wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatographic analysis of metabolic products, and in whole-cell analysis by pyrolysis-mass spectrometry (Py-MS). A numerical taxonomic approach was used with the results of conventional tests and the grouping obtained was compared with that obtained by Py-MS. All the ulcer and soft-tissue isolates and the urethritis isolates were oxidaseand urease-positive and formed a homogeneous set consistent with the reference strain of B. ureolyticus. The dental isolates differed from B. ureolyticus strains and were heterogeneous amongst themselves. None corresponded with the reference strains of B. gracilis or W . recta. The conventional and Py-MS approaches to c haracterisation produced similar groupings and each distinguished between a single cluster of ulcer-urethritis strains and several clusters of dental strains, although the dendrograms derived from the two approaches differed in the order of the clusters; in the Py-MS dendrogram one subcluster of four dental strains came within the main ulcer-urethritis cluster and a cluster of five ulcer strains was separated as a distinct group.

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“…Current schemes of classification are based mainly on the biochemical activities of the organisms (Holdeman and Moore, 1974;Holdeman, Cat0 and Moore, 1977). The strains used in the present study have been characterised carefully by the methods used in this laboratory, including gas-liquid chromatography (Duerden et al, 1976;Deacon et al, 1978;Duerden et al, 1980). The former subspecies of B. fragilis are now given separate species status, and B. thetaiotaomicron is further subdivided into the species B. thetaiotaomicron, B. eggerthii, and B. uniformis.…”

Section: Discussionmentioning

confidence: 99%

Neuraminidase production by Bacteroidaceae

Fraser¹,

Brown²

1981

Journal of Medical Microbiology

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SUMMARY. The production of neuraminidase (EC 3.2.1.18) by 77 strains of Bacteroidaceae was investigated by techniques previously used to study neuraminidase production by clostridia. Conditions for culture and assay of Bacteroides fragilis neuraminidase were characterised. The enzyme is predominantly cell associated; it is not calcium dependent and the pH optimum for its production is c. 4-5.Most neuraminidase-positive Bacteroides strains produced the enzyme well in the test media but a few strains failed to'produce it consistently in one or other of the media. Because of these occasional variations, strains were grown and tested in at least two media before being defined as neuraminidase negative.Within the B. fragilis group of species, B. fragilis, B. vulgatus, B. distasonis, B. ovatus, B. thetaiotaomicron and B. variabilis were neuraminidase positive while B. eggerthii, B. uniformis and B. splanchnicus were negative. Two subspecies of B. melaninogenicus (ss. melaninogenicus and ss. levii) were positive but the other (ss. intermedius) was negative. Strains of B. oralis and B. bivius produced the enzyme while B. ruminicola, B. disiens, B. asaccharolyticus and B. corrodens did not. The microaerophilic B. ochraceus were also positive. None of the Fusobacterium or Leptotrichia species tested produced neuraminidase.Our results for neuraminidase production are consistent for all strains of each species examined and we suggest that tests for neuraminidase production would be a valuable addition to biochemical tests currently used in taxonomic studies of the Bacteroidaceae.

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The Characterization of Clinically Important Gram Negative Anaerobic Bacilli by Conventional Bacteriological Tests

Cited by 74 publications

References 28 publications

The identification of gram-negative anaerobic bacilli isolated from clinical infections

The identification of gram-negative anaerobic bacilli isolated from clinical infections

A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Neuraminidase production by Bacteroidaceae

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