The Characterization of a Novel Phage, pPa_SNUABM_DT01, Infecting Pseudomonas aeruginosa

Abstract

The bacterial genus Pseudomonas is a common causative agent of infections in veterinary medicine. In this study, we focused on Pseudomonas aeruginosa canine otitis externa isolates. Due to prolonged antibiotic treatment of otitis externa, antibiotic resistance is common and has become a major complication. Many alternatives to antibiotics have been studied, with bacteriophages emerging as the most promising alternatives. Here, we isolated and characterized a novel phage, pPa_SNUABM_DT01, by investigating its morphology, growth, lysis kinetics, and genomic characteristics. Phages have a vigorous capacity to eliminate bacterial cells through bacterial lysis. This capacity is dependent on the multiplicity of infection (MOI), but even at low MOIs, the phage successfully inhibited bacterial regrowth. The phage genome was 265,520 bp in size and comprised 312 putative open reading frames (ORFs). Comparative genome analysis demonstrated that the phage is a novel species in Myoviridae. The nucleotide similarity was moderately high compared with the Pseudomonas virus, Noxifer. However, a phylogenetic analysis and a dot plot indicated that pPa_SNUABM_DT01 is not closely related to the Phikzvirus or Noxifervirus genus but, instead, belongs to a novel one. The genome comparisons also indicate that the phage, pPa_SNUABM_DT01, could be a novel genus.

The characterization and genome analysis of a novel phage phiA034 targeting multiple species of Aeromonas

“…Among the 82 ORFs annotated from the phage phiA034 genome, only 31 ORFs (37.8%) had known functions, and the rest were hypothetical proteins or unknown functions. Similar findings were reported by Hao (2016 ), Chanjuan (2013 ), Kwon et al. (2021 ), Deyuan et al.…”

The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism

“…In the TEM imaging of Phage K (Figure 1C), which belongs to the Myoviridae family genomically and morphologically; confirmed by the presence of an icosahedral shaped head, contractile sheathed tail, and a complex baseplate with knob‐like structures at the end of the tail. [ 51,73,74 ] In Figure 1D, the tail is contracted and the tail tube is clearly visible.…”

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

“…The experiment was performed similarly to those described elsewhere [ 38 ]. The lytic efficiency of the two new bacteriophages was determined using suspensions with different phage and bacteria ratios (at the most effective MOI determined in the previous experiment: 0.001, 0.01, 0.1, and 1.0).…”