The characterization and genome analysis of a novel phage phiA034 targeting multiple species of Aeromonas

Ye, Yingying; Tong, Guixiang; Chen, Gonghao; Huang, Lixing; Huang, Lei; Jiang, Xinglong; Wei, Xinxian; Lin, Min

doi:10.1016/j.virusres.2023.199193

Cited by 3 publications

(4 citation statements)

References 35 publications

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“…Phage phiA051 could only lyse A. hydrophila A009, A013, A019 and A051 out of 68 tested Aeromonas strains (including 24 strains of A. hydrophila ), implying that it has a narrow host range compare with some of phages that have been reported ( 38 , 51–59 ).…”

Section: Resultsmentioning

confidence: 93%

“…Water samples were taken from eel ponds in Fujian, China, aiming to detect phages against A. hydrophila A051, a strain isolated from diseased eels ( 38 ). The host A051 was inoculated into 100 mL of LB broth and incubated at 30°C, 150 rpm for 12 h to logarithmic phase, at which time the concentration of the bacterial suspension was approximately 10 9 CFU/mL.…”

Section: Methodsmentioning

confidence: 99%

“…The supernatant was filtered with a 0.22 μm filter membrane, and spot test method was applied to seek out phages according to the method from Zhang et al ( 39 ). Once a phage plaque had formed, a double-layer agar plate assay was used for confirmation and purification of the phage according to the method from Ye et al ( 38 ). Purification procedure was repeated 5 times.…”

Section: Methodsmentioning

confidence: 99%

“…Spot test was used to verify if the strain could be a host. Both strains source and test method were identical to those used by Ye et al ( 38 ). The formation of phage plaques confirmed the strain as a host.…”

Section: Methodsmentioning

confidence: 99%

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Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages

Wang,

Tong,

Jiang

et al. 2024

Front. Vet. Sci.

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IntroductionAeromonas hydrophila is particularly harmful to freshwater aquaculture, and the search for phage is an effective biological control method, but reports of possible temperate phages and their mutants are rare in this field. In this study, a virulent phage highly homologous to prophage in the genomes of A. hydrophila was collected and preliminary biological characterization was carried out to understand its nature.Materials and methodsWater samples taken from eel ponds in Fujian, China were combined with the strain. Spot test method and double-layer agar plate assay was used for confirmation and purification. Phage virions were observed using transmission electron microscope. A total of 68 strains of Aeromonas spp. were used to determine the host range. MOI groups of 1,000, 100, 10, 1, 0.1, 0.01, 0.001, 0.0001, 0.00001 were prepared to detect the optimal MOI. The conditions of thermal stability assay were set as 30, 40, 50, 60, 70 and 80°C for 1 h, respectively, and conditions of acid and alkali stability assay were set as 2.0, 4.0, 6.0, 8.0, 10.0 and 12.0 of pH. MOI of 0.01 and 0.1, respectively, are set to determine the inhibitory capacity of phage.ResultsA novel virulent A. hydrophila phage designated phiA051 has been isolated from aquaculture water. Electron microscopic observation showed that the phage phiA051 was composed of an icosahedral capsid. The phage phiA051 possesses an optimal multiplicity of infection (MOI) of 0.01, and its burst size was 108 PFU/cell. The phage maintained a high viability at temperatures of 30–50°C or pH 6.0–10.0 for 1 h. Phage phiA051 has certain potentials in rapidly inhibiting the spread of pathogen early in the outbreak, and it has a linear dsDNA with GC content of 60.55% and a total length of 32,212 bp, including 46 ORFs.DiscussionThe phage phiA051 behaved as a virulent phage. However, the BLASTN result showed that 23 of the top 25 hits were genomes of Aeromonas strains. It was suggested that phiA051 was probably derived from some prophage in the chromosome of Aeromonas. Further investigation of the mechanism how phage phiA051 transforms from a temperate phage to a virulent phage will provide a unique perspective and idea to explore the potential of prophages.

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Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages

Wang,

Tong,

Jiang

et al. 2024

Front. Vet. Sci.

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Targeted inactivation of multidrug-resistant Alcaligenes faecalis in pig farm WWTPs by mixed bacteriophages to diminish the risk of pathogenicity and antibiotic resistance dissemination

Cheng,

Zhang,

Liang

et al. 2024

Process Biochemistry

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Whole genome sequence analysis and characterization of lytic bacteriophages against antimicrobial-resistant diarrheagenic Escherichia coli strains isolated from various sources in Addis Ababa, Ethiopia

Sada,

Alemayehu,

Tafese

et al. 2024

Preprint

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The emergence of antibiotic resistance in E. coli strains has sparked a fervent investigation of alternative therapies such as the use of lytic bacteriophages. Phage whole genome sequencing is a novel method for learning more about proteins and other biomolecules encoded by phages, particularly phage lytic enzymes that are crucial to the lysis of bacterial cells. Seven potential lytic E. coli phages; EH-B-A (A1), EP-M-A, EP-B-K (E2), EI-SP-GF, ET-SD-TH, and ST-TK isolated from activated dairy farm sludges, Rivers, and hospital liquid waste were described. For sequencing, an Illumina NextSeq 550 sequencer was used. The virus nucleotide collection (nr/nt) (taxid:10239) was used to evaluate the whole genome sequences. Phylogenetic analysis was done using MEGA11 software. Genome sequencing revealed that each bacteriophage contains a linear double-stranded DNA genome. Phage isolates were taxonomically identified as 4 (57%) Myoviridae and 3 (43%) Siphoviridae phages. Phage genome length varied from 24264 to 143,710 bp, and their GC contents ranged from 43 to 54%. 33–218 CDSs (coding sequences) in total were predicted, with 19–77% of CDSs encoding functional proteins. All phages lacked tRNA in their genomes, except for EI-SP-GF, which possessed five tRNAs. Based on phylogenetic tree analysis, the phage isolates were related to Enterobacteria and E. coli phage sequences in the database. Screening did not show any genes encoding for a CRISPR-like system, virulence, antibiotic resistance, or lysogeny. Because of their stringent lytic nature, these phage isolates may be applied in the future to treat E. coli infections. This study may provide some primary data for the development of phage control techniques and advance our understanding of the genetic composition of E. coli phages.

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The characterization and genome analysis of a novel phage phiA034 targeting multiple species of Aeromonas

Cited by 3 publications

References 35 publications

Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages

Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages

Targeted inactivation of multidrug-resistant Alcaligenes faecalis in pig farm WWTPs by mixed bacteriophages to diminish the risk of pathogenicity and antibiotic resistance dissemination

Whole genome sequence analysis and characterization of lytic bacteriophages against antimicrobial-resistant diarrheagenic Escherichia coli strains isolated from various sources in Addis Ababa, Ethiopia

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