The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes

Meng, Ying‐Ying; Jiang, Junpeng; Wang, Haitao; Cao, Xupeng; Xue, Song; Yang, Qing; Wang, Weiliang

doi:10.1016/j.biortech.2014.12.012

Cited by 114 publications

(64 citation statements)

References 33 publications

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“…Nitrogen-induced changes in FA composition (high levels of C16:0 and C18:1 FAs with concomitantly reduced levels of PUFAs such as C20:5) support the proposal that de novo synthesised FAs (rather than converted membrane lipids) are directly incorporated into TAGs via the Kennedy pathway 42 . Similarly, in N-stressed N. gaditana , enhanced lipid biosynthesis was attributed to the transcriptional activation of cytosolic FA biosynthesis 16, 43, 44 .…”

Section: Discussionmentioning

confidence: 96%

“…Consistently, we observed weaker resistance to mechanical stress in cells with thickened cell walls compared with control cells, strongly suggesting that metabolic perturbations induced by N− could be a method to change the cell wall mechanical properties. Increasing the light intensity 45, 46 and medium salinity 11, 16, 43 during culture also enhanced cytosolic TAG accumulation in N. gaditana , most likely via transcriptional activation of the FAS1/PKS-like protein Kennedy pathway if the CO 2 level was not limiting 47–49 . Future work should evaluate whether light intensity and medium salinity, or a combination of these stress factors, also increases cell wall biosynthesis and results in cell wall thickening.…”

Section: Discussionmentioning

confidence: 97%

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Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

Jeong

Nam

Hwangbo

et al. 2017

Sci Rep

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Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N−), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N− stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis. However, few studies have characterised microalgal cell wall metabolism, including oleaginous Nannochloropsis sp. microalgae subjected to nitrogen deficiency. Here, we investigated N-induced changes in cellulose biosynthesis in N. salina. We observed that N− induced cell wall thickening, concurrently increased the transcript levels of genes coding for UDPG pyrophosphorylase and cellulose synthases, and increased cellulose content. Nannochloropsis salina cells with thickened cell wall were more susceptible to mechanical stress such as bead-beating and sonication, implicating cellulose metabolism as a potential target for cost-effective microalgal cell disruption.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

Jeong

Nam

Hwangbo

et al. 2017

Sci Rep

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“…The unicellular photosynthetic Nannochloropsis species (family Eustigmatophyceae) are considered promising oleaginous microalgae due to their rapid growth, high photosynthetic efficiency, and ability to produce large amounts of TAG (Rodolfi et al, 2009;Meng et al, 2015;Ma et al, 2016). The recently sequenced genomes and deep transcriptional profiling of several Nannochloropsis species aided by advances in genetic transformation methods have enabled increasing efforts to investigate and ultimately engineer Nannochloropsis metabolism (Radakovits et al, 2012;Vieler et al, 2012b;Li et al, 2014;Wang et al, 2014;Iwai et al, 2015;Poliner et al, 2015Poliner et al, , 2018aPoliner et al, , 2018bPoliner et al, , 2018cZienkiewicz et al, 2017).…”

mentioning

confidence: 99%

The Microalga Nannochloropsis during Transition from Quiescence to Autotrophy in Response to Nitrogen Availability

Zienkiewicz

Poliner

et al. 2019

Plant Physiol.

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“…Some of these microalgae species can accumulate lipids up to 60% of their dry weight (Hu et al, 2008), and for this reason, they have attracted attention as a possible feedstock for the production of biofuels. In this context, particular interest is raised by species of the Nannochloropsis genus, unicellular photosynthetic microalgae belonging to the Eustigmatophyceae and distributed worldwide in marine, fresh, and brackish waters, capable of massive lipid accumulation (Meng et al, 2015;Slocombe et al, 2015), especially under nutrient deprivation (Boussiba et al, 1987;Rodolfi et al, 2009).…”

mentioning

confidence: 99%

Light Remodels Lipid Biosynthesis in Nannochloropsis gaditana by Modulating Carbon Partitioning between Organelles

et al. 2016

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The seawater microalga Nannochloropsis gaditana is capable of accumulating a large fraction of reduced carbon as lipids. To clarify the molecular bases of this metabolic feature, we investigated light-driven lipid biosynthesis in Nannochloropsis gaditana cultures combining the analysis of photosynthetic functionality with transcriptomic, lipidomic and metabolomic approaches. Light-dependent alterations are observed in amino acid, isoprenoid, nucleic acid, and vitamin biosynthesis, suggesting a deep remodeling in the microalgal metabolism triggered by photoadaptation. In particular, high light intensity is shown to affect lipid biosynthesis, inducing the accumulation of diacylglyceryl-N,N,N-trimethylhomo-Ser and triacylglycerols, together with the up-regulation of genes involved in their biosynthesis. Chloroplast polar lipids are instead decreased. This situation correlates with the induction of genes coding for a putative cytosolic fatty acid synthase of type 1 (FAS1) and polyketide synthase (PKS) and the down-regulation of the chloroplast fatty acid synthase of type 2 (FAS2). Lipid accumulation is accompanied by the regulation of triose phosphate/inorganic phosphate transport across the chloroplast membranes, tuning the carbon metabolic allocation between cell compartments, favoring the cytoplasm, mitochondrion, and endoplasmic reticulum at the expense of the chloroplast. These results highlight the high flexibility of lipid biosynthesis in N. gaditana and lay the foundations for a hypothetical mechanism of regulation of primary carbon partitioning by controlling metabolite allocation at the subcellular level.

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The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes

Cited by 114 publications

References 33 publications

Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

The Microalga Nannochloropsis during Transition from Quiescence to Autotrophy in Response to Nitrogen Availability

Light Remodels Lipid Biosynthesis in Nannochloropsis gaditana by Modulating Carbon Partitioning between Organelles

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