THE CHARACTERISTICS OF BIOACTIVE PEPTIDES AND ANTIBACTERIAL ACTIVITY OF HONEY BEE (Apis nigrocincta) SMITH VENOM, ENDEMIC TO SULAWESI

Semuel, Mokosuli Yermia; Repi, Rudi Alexander

doi:10.20884/1.jm.2015.10.2.4

Cited by 3 publications

(2 citation statements)

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“…Moreover, the role of antibacterial in honey was already published (24,25). Antibacterial content of honey such as the acidity and non-peroxide activity is beneficial to reduce microorganism in wound surface (21,24,26,27). Therefore, honey may prevent the delayed wound healing process due to the unnecessary infection.…”

Section: Discussionmentioning

confidence: 99%

Effectivity of Indonesian Honey on Diabetic Foot Ulcers Healing Process: Observational Case Study

Sukarno

Hidayah²

2019

IJNHS

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Background: Diabetic foot ulcers were common diabetes complication that progressively growth globally. The effectivity of Manuka honey in wound healing process was huge published currently. Modern dressing and Manuka honey may not affordable and cost effective since these dressings were not produced originally in Indonesia. In addition, the published study of Indonesian honey is still lack. Therefore, we conducted this study to investigate the effectivity of Indonesian honey in diabetic foot ulcers healing process. Method: This study design was observational case study. This study was conducted among type 2 diabetes with diabetic foot ulcers in Griya Afiat Homecare and ETN Center in Makassar City, Indonesia from March to June 2014. The Bates Wound Assessment Tool (BWAT) were used to collect the score of diabetic foot ulcer healing process regarding wound size, depth, edges, undermining, necrotic tissue type, necrotic amount, granulation, epithelization, exudate type and amount, surrounding skin color, edema and induration. Descriptive, Independent t-test, Analysis of Variance (ANOVA) and Wilcoxon signed rank test were conducted appropriately. Results: In total, 10 type 2 diabetes patients with diabetic foot ulcers were participated. The factors associated with wound healing process were diabetes treatment (t= 2.44, p= 0.041) and primary dressing (t= -2.76, p= 0.025). The effect of honey primary dressing was in reducing wound size (p= 0.043), improving necrotic tissue type (p= 0.041), reducing necrotic tissue amount (p= 0.042), increasing granulation (p= 0.038) and epithelization (p= 0.042). In the counterpart, the effect of modern dressing was in improving necrotic tissue type (p= 0.046) and increasing granulation (p= 0.042). Conclusion: Indonesian honey is beneficial on diabetic foot ulcers healing process. Recommendation: Therefore, findings suggest that honey should be considered as the alternative, cost effective and beneficial wound dressing on the diabetic foot ulcers.

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Section: Discussionmentioning

confidence: 99%

Effectivity of Indonesian Honey on Diabetic Foot Ulcers Healing Process: Observational Case Study

Sukarno

Hidayah²

2019

IJNHS

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“…Sudah ada laporan penelitian pemanfaatan bagian sarang lebah madu Trigona spp sebagai antibakteri Streptococcus mutans (Mursalim & Jamaluddin, 2019) Prevelensi infeksi bakteri patogen terus meningkat dalam beberapa dekade ini diseluruh dunia. Perubahan iklim, transmisi antar hewan, resistensi antibiotik menyebabkan munculnya banyak species bakteri patogen baru pada manusia yang resisten terhadap antibiotik (Semuel et al, 2015) ; (Kanan et al, 2020); (Przybyłek & Karpiński, 2019). Oleh karena itu penelitian untuk mendapatkan sumber bioaktif antibiotik baru perlu terus menerus dilakukan.…”

Section: Pendahuluanunclassified

Antagonistic and Antibacterial Activity of Stapylocossus aureus and Isolates of Oral Bacteria from the Endogenous Fungus Apis dorsata Binghami Nest

Semuel

Wurarah

Tuegeh

2022

JPBN

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Apis Dorsata Binghami honey bee is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The research consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six isolates of fungi from the nest of Apis dorsata Binghami, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB2 and FAB3 had the best antagonist properties, while the FAB6 isolates had the weakest antagonist properties. The FAB2 isolate showed the best bacterial growth inhibition zone average for the isolates of oral bacteria and Staphylococcus aureus. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.

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The bioactive contents and antioxidant activity of honey bee nest extract of Apis dorsata Binghami from the North Sulawesi

Semuel

Kaunang²,

Manopo³

2019

Molekul

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Apis dorsata Binghami is a Sulawesi endemic honey bee. Apis dorsata Binghami cannot be bred, so it still lives wildlyin the forests of Sulawesi. However, Apis dorsata Binghami produces more honey, compared to all honey bee species. Furthermore, the diversity of plants as a source of nectar, pollen and plant resin, which is used in the formation of nests and honey is more, than all types of honey bees in the world. Ethnomedically, the Minahasa community has long used honeynest for degenerative diseases such as hyperlipidemia and cancer. Nevertheless, there have been no research reports on bioactive content and bioactivity of Apis dorsataBinghami nest extract. This research wasaimed to determinethe bioactive content of honey bee nest and to obtain the inhibitory concentration 50 (IC50) antioxidant activity of honey bee nest extractofApis dorsata Binghami. Honey bee nest was obtained directly from the forest of Minahasa peninsula, North Sulawesi, Indonesia. Extraction of fresh honey bee nest was conductedusingmaceration method. Bioactive content analysis was carried out by the Harborne method, followed by analysis using UV Vis spectrophotometer and High performance liquid chromatography. IC50antioxidant activity of honey bee nest extract was obtained using DPPH free radical reduction method. The results showed that Apis dorsata Binghami honey bee nest extract containedalkaloids, flavonoids, saponins, tannins, steroids and triterpenoids. Identified flavonoids displayed the highest phytochemical content. Based on the results of HPLC and UV Vis spectrophotometer analysis, there were 20 flavonoid derivatives found in honey bee nest samples in Minahasa. Ethanol extract and n-hexane extract showed high free radical reduction activity compared to vitamin C as a control treatment. However, ethanol extract produced the highest DPPH free radical reduction activity.

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THE CHARACTERISTICS OF BIOACTIVE PEPTIDES AND ANTIBACTERIAL ACTIVITY OF HONEY BEE (Apis nigrocincta) SMITH VENOM, ENDEMIC TO SULAWESI

Cited by 3 publications

References 1 publication

Effectivity of Indonesian Honey on Diabetic Foot Ulcers Healing Process: Observational Case Study

Effectivity of Indonesian Honey on Diabetic Foot Ulcers Healing Process: Observational Case Study

Antagonistic and Antibacterial Activity of Stapylocossus aureus and Isolates of Oral Bacteria from the Endogenous Fungus Apis dorsata Binghami Nest

The bioactive contents and antioxidant activity of honey bee nest extract of Apis dorsata Binghami from the North Sulawesi

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