The Changes of Rat Blood-Brain Barrier Permeability and Expression of Heat Shock Protein 70 after Infrasonic Damage

Fei, Zhou; Zhang, Xiang; Li, Zhigang; Lu, Pei-Ling; Liu, Xianzhen; Chen, Jingzao; Jia, Keyong

doi:10.1260/0263092001492840

Cited by 11 publications

(10 citation statements)

References 10 publications

(6 reference statements)

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“…Further evidence indicated that disrupted intracellular Ca 2? regulation may serve as a critical player responsible for the undesired biological outcomes following infrasound exposure [17]. The tightly controlled intracellular Ca 2?…”

Section: Discussionmentioning

confidence: 99%

“…For example, the inherent sound frequency of rat cardiac tissues is 5 Hz [1,16] similar to the infrasound frequency used in our experimental setting. Upon exposure to infrasound, sympathetic vibration may develop in internal organs, leading to direct physical damage and a series of secondary chemical injuries [1,17]. Recent finding has speculated that [Ca 2? ]…”

Section: Discussionmentioning

confidence: 99%

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Influence of Infrasound Exposure on the Whole L-type Calcium Currents in Rat Ventricular Myocytes

Pei¹,

Zhang²,

Xiao

et al. 2009

Cardiovasc Toxicol

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This study was designed to examine the effect of infrasound exposure (5 Hz at 130 dB) on whole-cell L-type Ca2+ currents (WLCC) in rat ventricular myocytes and the underlying mechanism(s) involved. Thirty-two adult Sprague-Dawley rats were randomly assigned to infrasound exposure and control groups. [Ca2+](i), WLCC, mRNA expression of the a(1c) subunit of L-type Ca2+ channels (LCC), and SERCA2 protein were examined on day 1, 7, and 14 after initiation of infrasound exposure. Fluo-3/AM fluorescence and the laser scanning confocal microscope techniques were used to measure [Ca2+](i) in freshly isolated ventricular myocytes. The Ca2+ fluorescence intensity (FI), denoting [Ca2+](i) in cardiomyocytes, was significantly elevated in a time-dependent manner in the exposure groups. There was a significant increase in WLCC in the 1-day group and a further significant increase in the 7- and 14-day groups. LCC mRNA expression measured by RT-PCR revealed a significant rise in the 1-day group and a significant additional rise in the 7- and 14-day groups compared with control group. SERCA2 expression was significantly upregulated in the 1-day group followed by an overt decrease in the 7- and 14-day groups. Prolonged exposure of infrasound altered WLCC in rat cardiomyocytes by shifting the steady-state inactivation curves to the right (more depolarized direction) without altering the slope and biophysical properties of I (Ca,L). Taken together, our data suggest that changes in [Ca2+](I) levels as well as expression of LCC and SERCA2 may contribute to the infrasound exposure-elicited cardiac response.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of Infrasound Exposure on the Whole L-type Calcium Currents in Rat Ventricular Myocytes

Pei¹,

Zhang²,

Xiao

et al. 2009

Cardiovasc Toxicol

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“…There are numerous studies using infrasound or ultrasound for transient BBB opening. 10,[34][35][36][37][38] At present, infrasound (8 Hz, 90-120 dB, duration -2h) effectively opens BBB but induces significant brain injuries due to increased intracellular calcium concentration, induction of apoptosis and high expression of heat shock protein 70. 34,35 The currently focused ultrasound is one of the most promising methods to enhance drug penetration into the brain by disruption the TJ between capillary endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…10,[34][35][36][37][38] At present, infrasound (8 Hz, 90-120 dB, duration -2h) effectively opens BBB but induces significant brain injuries due to increased intracellular calcium concentration, induction of apoptosis and high expression of heat shock protein 70. 34,35 The currently focused ultrasound is one of the most promising methods to enhance drug penetration into the brain by disruption the TJ between capillary endothelial cells. 10,[36][37][38] However, this method remains largely experimental and continues to make progress toward becoming a clinical tool.…”

Section: Discussionmentioning

confidence: 99%

Loud music and the specific sound stress open the blood-brain barrier: new fundamental, biomedical, and social aspects

Semyachkina-Glushkovskaya

Чехонин

Bragin³

et al. 2018

Preprint

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The blood-brain barrier (BBB) poses a significant challenge for drug brain delivery. The limitation of our knowledge about the nature of BBB explains the slow progress in the therapy of brain diseases and absence of methods for drug brain delivery in the clinical practice.Here we show that BBB opens for low/high weight molecules and nanocarriers after exposure of loud music/sound of 90 dB and 100 dB (regardless its frequency) as being easily produced by MP3/MP4 players, kitchen appliances, loudspeakers at concerts. The role of sound, sound-induced stress and molecular mechanisms behind is discussed in the framework of BBB opening as an informative platform for a novel fundamental knowledge about the nature of BBB and for the development of a non-invasive brain drug delivery technology.Social aspects of music/sound-induced opening of BBB provide completely new information about noise and healthy life conditions that will stimulate new research in this field.

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“…Accumulating evidence has demonstrated that infrasound of a certain sound pressure level (SPL), usually given in decibels (dB), may lead to dysfunction of the human body (2). Moreover, exposure to infrasound has been shown to induce damage to the central nervous system (CNS) in animal models (3)(4)(5)(6). However, the underlying mechanism remains to be clarified.…”

Section: Introductionmentioning

confidence: 99%

Infrasound increases intracellular calcium concentration and induces apoptosis in hippocampi of adult rats

Liu

2011

Mol Med Report

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Abstract. In the present study, we determined the effect of infrasonic exposure on apoptosis and intracellular free Ca 2+ ([Ca 2+ ] i ) levels in the hippocampus of adult rats. Adult rats were randomly divided into the control and infrasound exposure groups. For infrasound treatment, animals received infrasonic exposure at 90 (8 Hz) or 130 dB (8 Hz) for 2 h per day. Hippocampi were dissected, and isolated hippocampal neurons were cultured. The [Ca 2+ ] i levels in hippocampal neurons from adult rat brains were determined by Fluo-3/ AM staining with a confocal microscope system on days 1, 7, 14, 21 and 28 following infrasonic exposure. Apoptosis was evaluated by Annexin V-FITC and propidium iodide double staining. Positive cells were sorted and analyzed by flow cytometry. Elevated [Ca 2+ ] i levels were observed on days 14 and 21 after rats received daily treatment with 90 or 130 dB sound pressure level (SPL) infrasonic exposure (p<0.01 vs. control). The highest levels of [Ca 2+ ] i were detected in the 130 dB SPL infrasonic exposure group. Meanwhile, apoptosis in hippocampal neurons was found to increase on day 7 following 90 dB SPL infrasound exposure, and significantly increased on day 14. Upon 130 dB infrasound treatment, apoptosis was first observed on day 14, whereas the number of apoptotic cells gradually decreased thereafter. Additionally, a marked correlation between cell apoptosis and [Ca 2+ ] i levels was found on day 14 and 21 following daily treatment with 90 and 130 dB SPL, respectively. These results demonstrate that a period of infrasonic exposure induced apoptosis and upregulated [Ca 2+ ] i levels in hippocampal neurons, suggesting that infrasound may cause damage to the central nervous system (CNS) through the Ca 2+ -mediated apoptotic pathway in hippocampal neurons.

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The Changes of Rat Blood-Brain Barrier Permeability and Expression of Heat Shock Protein 70 after Infrasonic Damage

Abstract: To study the changes of blood-brain barrier (BBB)

Cited by 11 publications

References 10 publications

Influence of Infrasound Exposure on the Whole L-type Calcium Currents in Rat Ventricular Myocytes

Influence of Infrasound Exposure on the Whole L-type Calcium Currents in Rat Ventricular Myocytes

Loud music and the specific sound stress open the blood-brain barrier: new fundamental, biomedical, and social aspects

Infrasound increases intracellular calcium concentration and induces apoptosis in hippocampi of adult rats

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