The changes in pyroptosis-related inflammatory factors in the peripheral blood of patients with Henoch-Schonlein purpura

Abstract

Background: Henoch-Schonlein purpura (HSP) is a common capillary allergic bleeding disease. To explore the variation of pyroptosis-related inflammatory factors level in the peripheral blood of patients with HSP.Methods: A total of 87 HSP patients treated in our hospital from June 2020 to March 2021 were selected and divided into the renal impairment group (n=29) and the non-renal impairment group (n=58) according to the presence of hematuria and proteinuria. A total of 50 healthy individuals from the hospital were selected as the control group. The renal impairment and non-renal impairment groups were treated with a regular regimen of compound glycyrrhizin tablets and glucocorticoids, respectively. Serum interleukin (IL)-18, IL-1β, and peripheral caspase-1-positive cells were compared pre-and post-treatment among the three groups.Results: The pre-treatment serum IL-1β levels in the renal impairment and non-renal impairment groups were significantly higher than that in the control group (P<0.01). After treatment, the IL-1β level in the nonrenal impairment group was not significantly different from that in the control group (P>0.05). However, the IL-1β level in the renal impairment group post-treatment was significantly higher than that in the other two groups (P<0.01). The positive rate of caspase-1 expression in peripheral blood before treatment in the renal impairment group and non-renal impairment group was significantly higher than that in the control group (P<0.01). After treatment, the positive rate of caspase-1 expression in the non-renal impairment group was comparable to that in the control group (P>0.05), whereas the rate in the renal impairment group was significantly higher than that in the other two groups (P<0.01). After treatment, the serum IL-1β levels and caspase-1 positive rate in HSP patients who were responsive to treatment (as assessed by hematuria or proteinuria levels after treatment) were lower than that in patients who were unresponsive to treatment P<0.001), but not significantly different to the control group (P>0.05). Conclusions:The levels of serum IL-1β and caspase-1 changed in response to alterations in the disease condition and treatment response in HSP patients, which suggested that pyroptosis-related inflammatory factors may have potential application value in predicting disease progression and efficacy of hormone therapy.

Clinical application of compound Glycyrrhizin tablets in the treatment of patients with Simplex Henoch-Schonlein Purpura and its effect on immune function

“…Previous studies have also shown that the treatment regimen of compound glycyrrhizin tablets combined with glucocorticoids can significantly reduce the levels of febrile inflammatory factors in peripheral blood of patients with HSP without renal injury. 23 At the same time, 3 ineffective patients in the treatment group had no renal damage, while three of nine had no improvement in the control group had positive urine protein. Of course, owing to a limited sample size of the present study, it still remains unclear whether it is suggested that Compound Glycyrrhizin can reduce the incidence of renal damage in HSP.…”

Integrated analysis of immune- and apoptosis-related lncRNA-miRNA-mRNA regulatory network in children with Henoch Schönlein purpura nephritis

“…During the progression of HSPN, IgA deposition may induce apoptosis of human umbilical vein endothelial cells, thereby causing HSP vascular endothelial damage ( 35 ). On the other hand, apoptosis may promote the removal of inflammatory cells, contributing to the control of early inflammatory response and repair self-limiting vasculitis ( 36 ). Hence, there is a complex regulatory link between immune and apoptosis-associated signaling pathways in children with HSPN.…”

IgA vasculitis nephritis

“…This hypothesis is supported by a recent study that provided evidence that Il-1ß serum levels and the number of peripheral caspase-1-positive cells are increased in IgAV children. After prednisone treatment, Il-1ß and peripheral caspase-1-positive cells remained only elevated in the subgroup of patients who developed nephritis [48]. Pathophysiologically, amongst other functions, IL-1β upregulates the IL-2 receptor, prolongs T-cell survival and has a key role in B-cell proliferation, antibody production and the differentiation of Th17 cells into pro-inflammatory cells [49,50].…”