The challenges of introducing a new biofungicide to the market: A case study

Galindo, Enrique; Serrano‐Carreón, Leobardo; Gutiérrez, Carlos Roberto; Allende, Raúl; Balderas, Karina; Patino, M.E.; Trejo, Mario Hernández; Wong, Miguel Angel; Rayo, Emma; Isauro, Darío; Jurado, Carlos Del Valle

doi:10.2225/vol16-issue3-fulltext-6

Cited by 30 publications

(15 citation statements)

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“…Based on phylogenetic analysis of the 16S ribosomal genes, Bacillus strain 83 was initially classified as Bacillus subtilis (Galindo et al 2013 ). To review this classification in the light of genome sequence presented here, we performed pairwise whole-genome comparisons between this strain and other Bacillus species using the Average Nucleotide Identity (ANI) (Richter et al 2016 ).…”

Section: Resultsmentioning

confidence: 99%

“…B. velezensis 83, is an aerobic, gram positive, rod shaped and spore forming bacteria isolated by our research team from the mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. This strain is the biological component of Fungifree AB™ marketed in México since 2012, as a foliar fungicide, very effective for the biocontrol of five different genera of phytopathogenic fungi ( Colletotrichum, Erysiphe, Botrytis, Sphaerotheca and Leveillula) in crops of high agricultural importance such as mango, avocado, papaya, citrus, tomato, blueberry, blackberry, raspberry, zucchini, melon, cucumber, watermelon and others (Galindo et al 2013 ). Here, we reported in vivo assays to demonstrate the activity of this strain as BCA (mango) as well as PGPB (maize).…”

Section: Introductionmentioning

confidence: 99%

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Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

et al. 2020

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Bacillus velezensis 83 was isolated from mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. The assessment of this strain as BCA (biological control agent), as well as PGPB (plant growth-promoting bacteria), were demonstrated through in vivo and in vitro assays. In vivo assays showed that B. velezensis 83 was able to control anthracnose (Kent mangoes) as efficiently as chemical treatment with Captan 50 PH™ or Cupravit hidro™. The inoculation of B. velezensis 83 to the roots of maize seedlings yielded an increase of 12% in height and 45% of root biomass, as compared with uninoculated seedlings. In vitro co-culture assays showed that B. velezensis 83 promoted Arabidopsis thaliana growth (root and shoot biomass) while, under the same experimental conditions, B. velezensis FZB42 (reference strain) had a suppressive effect on plant growth. In order to characterize the isolated strain, the complete genome sequence of B. velezensis 83 is reported. Its circular genome consists of 3,997,902 bp coding to 3949 predicted genes. The assembly and annotation of this genome revealed gene clusters related with plant-bacteria interaction and sporulation, as well as ten secondary metabolites biosynthetic gene clusters implicated in the biological control of phytopathogens. Despite the high genomic identity (> 98%) between B. velezensis 83 and B. velezensis FZB42, they are phenotypically different. Indeed, in vitro production of compounds such as surfactin and bacillomycin D (biocontrol activity) and γ-PGA (biofilm component) is significantly different between both strains.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

et al. 2020

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“…Interestingly, some biological antagonists can survive on the leaves of plants, which allows spray application against foliar pathogens. As such, Bacillus subtilis is a rhizobacterium that has been widely tested for its production of antibiotics that affect the cell wall of plant pathogens [52]. In multiple greenhouse trials, the foliar application of B. subtilis with azoxystrobin provided the highest yield and the best disease control against powdery mildew (caused by Podosphaera xanthii) on zucchini, compared to both treatments alone [53].…”

Section: Combinations Of Fungicides With Biological Antagonistsmentioning

confidence: 99%

Combining Biocontrol Agents with Chemical Fungicides for Integrated Plant Fungal Disease Control

et al. 2020

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Feeding a rising population of currently 7.8 billion people globally requires efficient agriculture, which is preferably sustainable. Today, farmers are largely dependent on synthetic fungicides to avoid food losses caused by fungal diseases. However, the extensive use of these has resulted in the emergence of fungicide-resistant pathogens and concerns have been raised over the residual effects on the environment and human health. In this regard, biocontrol agents (BCAs) have been proposed as an alternative to standard fungicides but their disease management capacity is usually incomplete and heavily relies on uncontrollable environmental conditions. An integrated approach combining BCAs with fungicides, which is the focus of this review, is put forward as a way to reduce the fungicide doses to manage plant diseases and thereby their residue on harvested crops. In addition, such a strategy of combining antifungal treatments with different modes of action reduces the selection pressure on pathogens and thereby the chances of resistance development. However, to allow its large-scale implementation, further knowledge is needed, comprising timing, number and interval of repeated BCA applications and their compatibility with fungicides. The compatibility of BCAs with fungicides might differ when applied in a mixture or when used in alternation.

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“…In this regard, to strengthen the scientific research and enrich the functional range of bioproducts registered and sold in Mexico, it is essential to incorporate new cutting-edge and robust tools into scientific studies of BCA-M, especially about their polyphasic taxonomic affiliation, action mode in vitro, and under greenhouse and field conditions, as well as their interaction with biotic and abiotic factors. This would lead to the establishment of cost-effective production, formulation, scaling and innovation systems (Galindo et al, 2013;Serrano-Carreón et al, 2010), which, in turn, would help governmental agencies and professionals involved in agricultural production make an efficient, safe, and rigorous technology transfer of these bioproducts to farmers. Taxonomic affiliation of BCA-M.…”

Section: Fully Bilingualmentioning

confidence: 99%

Omics sciences potential on bioprospecting of biological control microbial agents: the case of the Mexican agro-biotechnology

Córdova-Albores¹,

Zelaya-Molina²,

Ávila-Alistac³

et al. 2020

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Actualmente, los estudios sobre agentes de control biológico de origen microbiano (ACB-M) generalmente están enfocados en la caracterización taxonómica mediante el uso de marcadores moleculares convencionales y en evaluar la capacidad antagónica/mecanismos de acción in vitro, en invernadero y eventualmente bajo condiciones de campo. Los ACB-M se centran principalmente en cepas de Trichoderma, Paecilomyces, Beauveria, Pseudomonas y Bacillus. Aunque la investigación en México en este campo ha sido muy activa en los últimos años, el desarrollo e innovación de una mayor diversidad de bioplaguicidas registrados y comercializados puede ser potenciada. En este contexto, el uso de técnicas vanguardistas en la era de las ciencias ómicas (genómica, transcriptómica, y metabolómica) enfocadas a la correcta afiliación taxonómica de los ACB-M y en el estudio de mecanismos de acción y comportamiento agroecológico es determinante para la bioprospección y uso extensivo de estos ACB-M de manera eficaz, biosegura y costo-efectiva. En el marco de la celebración internacional de la sanidad vegetal, la presente revisión analiza críticamente el estado del conocimiento y de aquellos aspectos que limitan la bioprospección y el uso extensivo de ACB-M con énfasis en México, desde la aplicación de las ciencias ómicas para la identificación, selección y estudio de los mecanismos de acción de dichos agentes hasta la difusión y socialización del conocimiento científico generado. Se pretende promover la reflexión sobre este campo del conocimiento e incentivar la nueva generación ACB-M con una visión holística y sistémica en beneficio de una agricultura sustentable y resiliente.

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The challenges of introducing a new biofungicide to the market: A case study

Cited by 30 publications

References 14 publications

Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

Combining Biocontrol Agents with Chemical Fungicides for Integrated Plant Fungal Disease Control

Omics sciences potential on bioprospecting of biological control microbial agents: the case of the Mexican agro-biotechnology

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