Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion

Abstract

Bacillus velezensis 83 was isolated from mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. The assessment of this strain as BCA (biological control agent), as well as PGPB (plant growth-promoting bacteria), were demonstrated through in vivo and in vitro assays. In vivo assays showed that B. velezensis 83 was able to control anthracnose (Kent mangoes) as efficiently as chemical treatment with Captan 50 PH™ or Cupravit hidro™. The inoculation of B. velezensis 83 to the roots of maize seedlings yielded an increase of 12% in height and 45% of root biomass, as compared with uninoculated seedlings. In vitro co-culture assays showed that B. velezensis 83 promoted Arabidopsis thaliana growth (root and shoot biomass) while, under the same experimental conditions, B. velezensis FZB42 (reference strain) had a suppressive effect on plant growth. In order to characterize the isolated strain, the complete genome sequence of B. velezensis 83 is reported. Its circular genome consists of 3,997,902 bp coding to 3949 predicted genes. The assembly and annotation of this genome revealed gene clusters related with plant-bacteria interaction and sporulation, as well as ten secondary metabolites biosynthetic gene clusters implicated in the biological control of phytopathogens. Despite the high genomic identity (> 98%) between B. velezensis 83 and B. velezensis FZB42, they are phenotypically different. Indeed, in vitro production of compounds such as surfactin and bacillomycin D (biocontrol activity) and γ-PGA (biofilm component) is significantly different between both strains.

Characterization of Soil Bacteria with Potential to Degrade Benzoate and Antagonistic to Fungal and Bacterial Phytopathogens

“…B. subtilis ACB-83 produced two antibiotics, iturin and surfactin, and was successfully used to prevent and control citrus black spots caused by the fungus Phyllosticta citricarpa [ 39 ]. Bacillus velezensis 83 was isolated from the mango tree phyllosphere of orchards [ 9 ]. This strain can be listed as an example of the ability of bacilli to carry out a protective function, synthesizing compounds that can inhibit the growth of phytopathogens and acting as producers of compounds that stimulate plant growth and development.…”

“…Among the research fields of soil microbiology, it is necessary to note such areas as the assessment of the general state of microbial systems, seasonal fluctuations in the biomass of the microbiocenosis and the spread of microorganisms of various taxonomic groups in soils of different types [ 7 ], as well as the study of the effect of the anthropogenic load on the change in abundance and the diversity of soil microorganisms [ 8 ]. Special attention is paid to the study of rhizosphere microorganisms, including bacteria that stimulate plant growth (plant growth-promoting rhizosphere bacteria) [ 9 , 10 ]. In this case, not only microbial diversity is investigated, but the features of the interactions of microorganisms with the host plant are also revealed.…”

A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens

“…The ability to produce endospores when facing harsh conditions allowed the members of the operational group to survive in various niches including soil, animal faeces, plants, food, bee products, drugs, air, and the aquatic environments ( Table S1 ). Evidently, the members of OG Ba had been directly isolated from rare dormant volcanic soils [ 19 ], mango orchards [ 20 ] and animal faeces [ 21 , 22 ]. They had also been isolated from plant parts including fruits (such as lemons [ 23 ] and apples [ 24 ]), roots (such as Peruvian ground cherry [ 25 ] and peanut roots [ 26 ]) and leaves (such as lucerne [ 27 ] and camphor leaves [ 28 ]).…”

New Strains of Bacteria that Degrade Aromatic Compounds Act as Antagonists of Highly Active Phytopathogens

“…Among the research fields of soil microbiology, it is necessary to note such areas as the assessment of the general state of microbial systems, the seasonal fluctuations in the biomass of microbiocenosis, the spread of microorganisms of various taxonomic groups in soils of different types [2], as well as the study of the effect of anthropogenic load on the change in abundance and the diversity of soil microorganisms [3]. Special attention is paid to the study of rhizosphere microorganisms, including bacteria that stimulate plant growth (plant-growth-promoting rhizosphere bacteria) [4,5]. In this case, not only microbial diversity is investigated, but also the features of the interaction of microorganisms with the host plant are revealed.…”