The Cellular Uptake, Distribution and Toxicity of Poly (lactic-co-glycolic) Acid Nanoparticles in Medicago sativa Suspension Culture

Ulusoy, Esma; Derman, Serap; Erişen, Semiha

doi:10.25083/rbl/25.3/1572.1580

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Particularly, in the primary wall, pectins are known to regulate porosity through the galactan and arabinan side chains of rhamnogalacturonan [32]. TEM analyses conducted on M. sativa suspension cultures showed that PLGA NPs passed through the cell wall and accumulated into the cytoplasm and nucleus [33]. After crossing the cell wall, NPs then cross the membrane by endocytosis [34], a process involving the internalization of extracellular materials or plasma membrane proteins into the cell through a series of vesicular compartments [35].…”

Section: Discussionmentioning

confidence: 99%

Confocal Microscopy Investigations of Biopolymeric PLGA Nanoparticle Uptake in Arabidopsis thaliana L. Cultured Cells and Plantlet Roots

Angelis

Badiali

Chronopoulou

et al. 2023

Plants

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To date, most endocytosis studies in plant cells have focused on clathrin-dependent endocytosis, while limited evidence is available on clathrin-independent pathways. Since dynamin a is a key protein both in clathrin-mediated endocytosis and in clathrin-independent endocytic processes, this study investigated its role in the uptake of poly-(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs). The experiments were performed on cultured cells and roots of Arabidopsis thaliana. Dynasore was used to inhibit the activity of dynamin-like proteins to investigate whether PLGA NPs enter plant cells through a dynamin-like-dependent or dynamin-like-independent endocytic pathway. Observations were performed by confocal microscopy using a fluorescent probe, coumarin 6, loaded in PLGA NPs. The results showed that both cells and roots of A. thaliana rapidly take up PLGA NPs. Dynasore was administered at different concentrations and exposure times in order to identify the effective ones for inhibitory activity. Treatments with dynasore did not prevent the NPs uptake, as revealed by the presence of fluorescence emission detected in the cytoplasm. At the highest concentration and the longest exposure time to dynasore, the fluorescence of NPs was not visible due to cell death. Thus, the results suggest that, because the NPs’ uptake is unaffected by dynasore exposure, NPs can enter cells and roots by following a dynamin-like-independent endocytic pathway.

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Section: Discussionmentioning

confidence: 99%

Confocal Microscopy Investigations of Biopolymeric PLGA Nanoparticle Uptake in Arabidopsis thaliana L. Cultured Cells and Plantlet Roots

Angelis

Badiali

Chronopoulou

et al. 2023

Plants

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“…Likewise, in vitro tests proved that PLGA nanocarriers are non‐phytotoxic for plant cells (but were not tested herein). [ 45 ]…”

Section: Simulation Of In Planta Conditions By Wood Sapsmentioning

confidence: 99%

Targeted Drug Delivery for Sustainable Crop Protection: Transport and Stability of Polymeric Nanocarriers in Plants

et al. 2021

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Spraying of agrochemicals (pesticides, fertilizers) causes environmental pollution on a million‐ton scale. A sustainable alternative is target‐specific, on‐demand drug delivery by polymeric nanocarriers. Trunk injections of aqueous nanocarrier dispersions can overcome the biological size barriers of roots and leaves and allow distributing the nanocarriers through the plant. To date, the fate of polymeric nanocarriers inside a plant is widely unknown. Here, the in planta conditions in grapevine plants are simulated and the colloidal stability of a systematic series of nanocarriers composed of polystyrene (well‐defined model) and biodegradable lignin and polylactic‐co‐glycolic acid by a combination of different techniques is studied. Despite the adsorption of carbohydrates and other biomolecules onto the nanocarriers’ surface, they remain colloidally stable after incubation in biological fluids (wood sap), suggesting a potential transport via the xylem. The transport is tracked by fluorine‐ and ruthenium‐labeled nanocarriers inside of grapevines by 19F‐magnetic resonance imaging or induced coupled plasma – optical emission spectroscopy. Both methods show that the nanocarriers are transported inside of the plant and proved to be powerful tools to localize nanomaterials in plants. This study provides essential information to design nanocarriers for agrochemical delivery in plants to sustainable crop protection.

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The Cellular Uptake, Distribution and Toxicity of Poly (lactic-co-glycolic) Acid Nanoparticles in Medicago sativa Suspension Culture

Cited by 2 publications

References 28 publications

Confocal Microscopy Investigations of Biopolymeric PLGA Nanoparticle Uptake in Arabidopsis thaliana L. Cultured Cells and Plantlet Roots

Confocal Microscopy Investigations of Biopolymeric PLGA Nanoparticle Uptake in Arabidopsis thaliana L. Cultured Cells and Plantlet Roots

Targeted Drug Delivery for Sustainable Crop Protection: Transport and Stability of Polymeric Nanocarriers in Plants

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