THE CELLULAR TRANSFER OF IMMUNITY TO TRICHOSTRONGYLUS COLUBRIFORMIS IN AN ISOGENIC STRAIN OF GUINEA‐PIG

Abstract

Summary The mechanism of resistance of guinea‐pigs to infection with Trichostrongylus colubrifomis was investigated by transferring serum and mesenteric lymph nodes from resistant guinea‐pigs to susceptible guinea‐pigs of the same inbred strain. Inbred guinea‐pigs were used in these studies to avoid destruc tion of the transferred tissues by a homograft reaction. It was shown that the transfer of mesenteric lymph nodes prevented a primary infection from reaching patency in recipient guinea‐pigs, whereas a single injection of serum from resistant donors did not have any observable effect on the development of primary infection. It is concluded that the failure of susceptible guinea‐pigs to become infected with T. colubriformis was due to an immunological reaction in which cellular factors were more important than humoral antibodies. It is suggested that control of T. colubriformis and other helminth parasites with all stages confined to the alimentary tract may be mediated by a delayed type of hypersensitive reaction.

Immunity to Helminths: Resistance, Regulation, and Susceptibility to Gastrointestinal Nematodes

“…There was also a suggestion that a cellular compartment may be involved in protection, although exactly what cells were important and how they contributed were unclear (16). This idea was extended several decades later through a series of papers from Ogilvie and colleagues (22)(23)(24), again working with N. brasiliensis in the rat, together with studies from Dineen and colleagues (25)(26)(27) using the ovine parasite Trichostrongylus colobriformis, in this case a sheep parasite adapted to the guinea pig. Ogilvie and coworkers developed the idea in the 1960s and 1970s of protective immunity operating through antibody and cells-lymphocytes-and also identified [alongside other groups, e.g., Jarrett (28,29)] the highly elevated IgE levels that were associated with worm infection.…”

Immunity: Host Response

“…Wagland and Dineen (1965) were able to transfer protective immunity to this parasite in guinea pigs with mesenteric lymph node cells but not with immune serum. Studies by Dineen and coworkers on the response to Trichostrongylus colubriformis, a sheep intestinal trichostrongyle, adapted to the guinea pig, have added substantial understanding to the immune mechanisms to parasitic nematodes.…”

Killing Effect of Normal Peritoneal Exudate Cells in Guinea Pigs on Microfilariae of Dirofilaria Immitis Evoked by Passive Transfer of Immune Humoral Factor

“…In in vivo systems, however, successful transfer of immunity against parasites by use of not the immune serum but the lymphoid cells has often been reported in infections with such helminths as Trichinella spiralis (Lash et al, 1966), Trichostrongylus colubri f ormis (Wagland and Dineen, 1965), strongylus brasiliensis (Kelly and Dineen, 1972) and Hymenolepsis nana (Friedberg et al, 1967). In addition, suppression of the acquired immunity or prolongation of the infection course by the treatment with anti-thymocyte serum (Okamoto and Koizumi, 1972), anti-lymphocyte serum (DiNetta et al, 1972) or neonatal thymectomy (Okamoto, 1970) has also been reported.…”