Killing Effect of Normal Peritoneal Exudate Cells in Guinea Pigs on Microfilariae of Dirofilaria Immitis Evoked by Passive Transfer of Immune Humoral Factor

Kobayakawa, Takatoshi; Shinbo, Shun; Ishiyama, Hiroshi; Masuda, Kazushige

doi:10.7883/yoken1952.29.351

JJMSB

1976

DOI: 10.7883/yoken1952.29.351

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Killing Effect of Normal Peritoneal Exudate Cells in Guinea Pigs on Microfilariae of Dirofilaria Immitis Evoked by Passive Transfer of Immune Humoral Factor

Takatoshi Kobayakawa¹,

Shun Shinbo²,

Hiroshi Ishiyama

et al.

Abstract: SUMMARY : Parasiticidal activity of normal peritoneal exudate cells on microfilariae of Diro fclaria immitis in diffusion chambers implanted into normal guinea pigs was evoked by intraperitoneal passive transfer of anti-D. immitis serum. The immune serum was fractionated into supernatant and sediment by ammonium sulfate precipitation.The parasiticidal effect was reproduced with the supernatant and, to a less extent, with the sediment. Anti-D. immitis serum from the animals sensitized 5 days before was also eff… Show more

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1987

1988

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Cited by 2 publications

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Active and Passive Immunization of Mice against Larval Dirofilaria immitis

Abraham

Grieve²,

Mika-Grieve³

et al. 1988

The Journal of Parasitology

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The objective of this study was to determine if Dirofilaria immitis larvae would survive in diffusion chambers implanted in dogs and mice and secondly to determine if mice could be immunized against infection with D. immitis. Dirofilaria immitis third-stage larvae (L3) survived and grew in diffusion chambers implanted in dogs and mice for at least 3 wk. BALB/c mice, which were repeatedly infected with live L3, showed resistance to challenge infections. Dead L3, with or without adjuvants elicited no protective immunity. A correlation was found between the degree of immune protection seen in mice and antibody levels to soluble larval antigen but not to antibody levels to surface antigens. A monoclonal antibody was prepared that reacted with the surface of D. immitis and Onchocerca lienalis L3, but not to the surfaces of other stages and species of various filarial worms. When this antibody was administered to mice prior to challenge no significant reduction in larval survival was observed.

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Active and Passive Immunization of Mice against Larval Dirofilaria immitis

Abraham

Grieve²,

Mika-Grieve³

et al. 1988

The Journal of Parasitology

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show abstract

Passive transfer of immunity against Clonorchis sinensis by peritoneal exudate cells in mice

Kwon

Kang

Choi³

1987

Korean J Parasitol

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This study was undertaken to evaluate the role of peritoneal exudate cells in the transfer of immunity against the liver fluke, Clonorchis sinensis in the inbred BALB/c mice. Ten donor mice were divided into 2 groups. One group consisted of 5 mice was infected orally with 20 metacercariae of C. sinensis, and the other group was injected intraperitoneally with 20 excysted larvae. Thirty days after immunization, the peritoneal exudate cells were obtained from the donor mice. Twenty recipient mice were divided into 4 equal groups for the purpose of primary immunization. The mice of Group I were injected intraperitoneally with 2 x 10(6) peritoneal exudate cells of the donor mice infected orally, those of Group III were injected intraperitoneally with 2 x 10(6) peritoneal exudate cells of the donor mice injected intraperitoneally. Those of Group II were injected orally with 20 metacercariae of C. sinensis. The group IV mice served as controls. Four days after the primary immunization all recipient mice were challenged orally with 20 metacercariae of C. sinensis, and then killed 30 days after the challenging infection. When the peritoneal exudate cells were injected into the recipient mice, pronounced reduction in eggs per gram of the feces was found in the mice of Group I and Group II, but no reduction in those of Group III. In the worm burdens of C. sinensis, the number of flukes found in the mice of Group II was only significantly less than those in the control group(IV). In addition the number of plaque forming cells per spleen in the mice of Group II was found larger than those in Group I. It is likely that donor peritoneal exudate cells transferred to the recipients might result in the production of relative immunity.

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Killing Effect of Normal Peritoneal Exudate Cells in Guinea Pigs on Microfilariae of Dirofilaria Immitis Evoked by Passive Transfer of Immune Humoral Factor

Cited by 2 publications

References 10 publications

Active and Passive Immunization of Mice against Larval Dirofilaria immitis

Active and Passive Immunization of Mice against Larval Dirofilaria immitis

Passive transfer of immunity against Clonorchis sinensis by peritoneal exudate cells in mice

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