The cellular slime mold guild and its bacterial prey: growth rate variation at the inter- and intraspecific levels

Eisenberg, Robert M.; Hurd, Lawrence E.; Ketcham, Robert B.

doi:10.1007/bf00378661

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Given their global distribution, it is not surprising that dictyostelids can feed on a wide range of bacteria (Raper 1937; Raper and Smith 1939). Food “preferences” and competitive advantages amongst dictyostelid isolates have been observed (Eisenberg et al 1989; Horn 1971; Ketcham et al 1988; Ketcham and Eisenberg 1989). Experiments profiling gene expression during D. discoideum feeding on Gram positive or Gram negative bacteria suggest prey and predators communicate with each other using chemical cues (Carilla-Latorre et al 2008; Nasser et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species

Sanders

Borys

Kisa

et al. 2017

Protist

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Dictyostelids are free-living phagocytes that feed on bacteria in diverse habitats. When bacterial prey is in short supply or depleted, they undergo multicellular development culminating in the formation of dormant spores. In this work, we tested isolates representing four dictyostelid species from two genera (Dictyostelium and Polysphondylium) for the potential to feed on biofilms preformed on glass and polycarbonate surfaces. The abilities of dictyostelids were monitored for three hallmarks of activity: 1) spore germination on biofilms, 2) predation on biofilm enmeshed bacteria by phagocytic cells and 3) characteristic stages of multicellular development (streaming and fructification). We found that all dictyostelid isolates tested could feed on biofilm enmeshed bacteria produced by human and plant pathogens: Klebsiella oxytoca, Pseudomonas aeruginosa, Pseudomonas syringae, Erwinia amylovora 1189 (biofilm former) and E. amylovora 1189 Δams (biofilm deficient mutant). However, when dictyostelids were fed planktonic E. amylovora Δams the bacterial cells exhibited an increased susceptibility to predation by one of the two dictyostelid strains they were tested against. Taken together, the qualitative and quantitative data presented here suggest that dictyostelids have preferences in bacterial prey which affects their efficiency of feeding on bacterial biofilms.

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Section: Introductionmentioning

confidence: 99%

Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species

Sanders

Borys

Kisa

et al. 2017

Protist

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“…These cells phagocytose bacteria for food, but they are also infected and killed by some bacteria and colonized by others [2,4]. Early work showed that dictyostelids are limited in the soil by bacterial availability, and they show intra-and interspecific variation for growth on co-occurring bacteria [5][6][7]. Other work identified distinct sets of genes that regulate growth on Gram-negative versus Gram-positive bacteria, as well as physiological changes that take place upon phagocytosis of these two types of bacteria [8,9].…”

Section: Introductionmentioning

confidence: 99%

Phagocyte chase behaviours: discrimination between Gram-negative and Gram-positive bacteria by amoebae

Rashidi

Ostrowski

2019

Biol. Lett.

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Phagocytes are cells that pursue, engulf and kill bacteria. They include macrophages and neutrophils of the mammalian immune system, as well as free-living amoebae that hunt and engulf bacteria for food. Phagocytosis can result in diverse outcomes, ranging from sustenance to infection and colonization by either pathogens or beneficial symbionts-and thus, discrimination may be necessary to seek out good bacteria while avoiding bad ones.Here we tested whether the soil amoeba Dictyostelium discoideum can discriminate among different types of bacteria using behavioural assays where amoebae were presented with paired choices of different bacteria. We observed variation in the extent to which the amoebae pursued different types of bacteria, as well as preferential migration towards Gram-negative compared with Gram-positive bacteria. Response profiles were similar for amoebae that originated from different geographical locations, suggesting that chase preference is conserved across much of the species range. While prior work has demonstrated that bacteria use chemotaxis to seek out amoebae they colonize, our work suggests that the opposite also occursamoebae can preferentially direct themselves to particular bacteria in the environment. Preferential sensing and response may help to explain why some amoeba-bacteria associations are more common in nature than others.

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The Ecology and Evolution of Amoeba-Bacterium Interactions

Shi

Queller

Tian

et al. 2021

Appl Environ Microbiol

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Amoebae are protists that have complicated relationships with bacteria, which cover the whole spectrum of symbiosis. Amoeba-bacteria interactions contribute to the study of predation, symbiosis, pathogenesis, and human health. Given the complexity of their relationships, it is necessary to understand the ecology and evolution of their interactions. In this paper, we provide an updated review of the current understanding of amoeba-bacteria interactions. We start by discussing the diversity of amoebae and their bacterial partners. Besides, we define three types of ecological interactions between amoebae and bacteria and discuss their different outcomes. Finally, we focus on the implications of amoeba-bacteria interactions on human health, horizontal gene transfer, drinking water safety, and the evolution of symbiosis. In conclusion, amoeba-bacteria interactions are excellent model systems to investigate a wide range of scientific questions. Future studies should utilize advanced techniques to address research gaps such as detecting hidden diversity, lack of amoebae genome, and the impacts of amoeba predation on the microbiome.

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The cellular slime mold guild and its bacterial prey: growth rate variation at the inter- and intraspecific levels

Cited by 4 publications

References 15 publications

Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species

Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species

Phagocyte chase behaviours: discrimination between Gram-negative and Gram-positive bacteria by amoebae

The Ecology and Evolution of Amoeba-Bacterium Interactions

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