Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species

Sanders, Dean; Borys, Katarzyna D.; Kisa, Fikrullah; Rakowski, Sheryl A.; Lozano, Marcela; Filutowicz, Marcin

doi:10.1016/j.protis.2017.04.001

Cited by 6 publications

(6 citation statements)

References 107 publications

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“…The strains used in this study belong to species known for their ability to form biofilms: most notably, Hafnia sp, Serratia sp, and Klebsiella oxytoca [30][31][32]. As expected, it was two strains of Hafnia, namely, H. alvei strain 37 and H. paralvei strain 43, that had the best ability to form a biofilm, which is in concordance with the relevant scientific literature concerning genetically close strains [30].…”

Section: Discussionsupporting

confidence: 84%

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Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations

Radulović

Stanković

Uzelac

et al. 2020

Plants

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The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.

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Section: Discussionsupporting

confidence: 84%

“…The fact that both Klebsiella oxytoca strains used in this study (strain 9 and 14) formed biofilms in vitro, but only one (strain 14) formed intimate connections with the root system of duckweed, implied a higher level of biological specificity of the tested bacteria-plant interactions, which is also supported by similar studies [32,34].…”

Section: Discussionsupporting

confidence: 83%

Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations

Radulović

Stanković

Uzelac

et al. 2020

Plants

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“…The observation that D . discoideum secretes factors that mediate the degradation of bacterial biofilms supports and extends these conclusions [43]. We also found evidence for cheating during cooperative predation, which supports our conclusion since cheating is common in cooperative biological systems.…”

Section: Discussionsupporting

confidence: 89%

Cooperative predation in the social amoebae Dictyostelium discoideum

et al. 2019

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The eukaryotic amoeba Dictyostelium discoideum is commonly used to study sociality. The amoebae cooperate during development, exhibiting altruism, cheating, and kin-discrimination, but growth while preying on bacteria has been considered asocial. Here we show that Dictyostelium are cooperative predators. Using mutants that grow poorly on Gram-negative bacteria but grow well on Gram-positive bacteria, we show that growth depends on cell-density and on prey type. We also found synergy, by showing that pairwise mixes of different mutants grow well on live Gram-negative bacteria. Moreover, wild-type amoebae produce diffusible factors that facilitate mutant growth and some mutants exploit the wild type in mixed cultures. Finding cooperative predation in D. discoideum should facilitate studies of this fascinating phenomenon, which has not been amenable to genetic analysis before.

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“…The lipopolysaccharides (complexes of crystalline cellulose with lipids) synthesized by gram negative bacteria (proteobacteria, actinobacteria) and fungi, too, could aid protection of the organism from host immune systems (phagocytosis) while concomitantly establishing an infection ( Cryptococcus neoformans, Pseudomonas spp., Vibrio spp.) or infestation in developing protists and marine invertebrates [ 9 – 14 , 41 , 43 , 48 , 102 – 104 ]. Reciprocally, an interesting utility of GH9 endoglucanases is to facilitate the symbiotic/ parasitic association between some fungi and bacteria of animal and plants hosts (macrophages, leguminous nodules of the rhizomes) by digesting the crystalline cellulose of the host.…”

Section: Discussionmentioning

confidence: 99%

Origin, evolution, and divergence of plant class C GH9 endoglucanases

Kundu

Sharma

2018

BMC Evol Biol

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BackgroundGlycoside hydrolases of the GH9 family encode cellulases that predominantly function as endoglucanases and have wide applications in the food, paper, pharmaceutical, and biofuel industries. The partitioning of plant GH9 endoglucanases, into classes A, B, and C, is based on the differential presence of transmembrane, signal peptide, and the carbohydrate binding module (CBM49). There is considerable debate on the distribution and the functions of these enzymes which may vary in different organisms. In light of these findings we examined the origin, emergence, and subsequent divergence of plant GH9 endoglucanases, with an emphasis on elucidating the role of CBM49 in the digestion of crystalline cellulose by class C members.ResultsSince, the digestion of crystalline cellulose mandates the presence of a well-defined set of aromatic and polar amino acids and/or an attributable domain that can mediate this conversion, we hypothesize a vertical mode of transfer of genes that could favour the emergence of class C like GH9 endoglucanase activity in land plants from potentially ancestral non plant taxa. We demonstrated the concomitant occurrence of a GH9 domain with CBM49 and other homologous carbohydrate binding modules, in putative endoglucanase sequences from several non-plant taxa. In the absence of comparable full length CBMs, we have characterized several low strength patterns that could approximate the CBM49, thereby, extending support for digestion of crystalline cellulose to other segments of the protein. We also provide data suggestive of the ancestral role of putative class C GH9 endoglucanases in land plants, which includes detailed phylogenetics and the presence and subsequent loss of CBM49, transmembrane, and signal peptide regions in certain populations of early land plants. These findings suggest that classes A and B of modern vascular land plants may have emerged by diverging directly from CBM49 encompassing putative class C enzymes.ConclusionOur detailed phylogenetic and bioinformatics analysis of putative GH9 endoglucanase sequences across major taxa suggests that plant class C enzymes, despite their recent discovery, could function as the last common ancestor of classes A and B. Additionally, research into their ability to digest or inter-convert crystalline and amorphous forms of cellulose could make them lucrative candidates for engineering biofuel feedstock.Electronic supplementary materialThe online version of this article (10.1186/s12862-018-1185-2) contains supplementary material, which is available to authorized users.

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Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species

Cited by 6 publications

References 107 publications

Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations

Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations

Cooperative predation in the social amoebae Dictyostelium discoideum

Origin, evolution, and divergence of plant class C GH9 endoglucanases

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