The cellular level of prostatic acid phosphatase and the growth of human prostate carcinoma cells

Lin, Ming Fong; Garcia-Arenas, Renee; Xia, Xing; Biela, Barbara H.; Lin, Fen Fen

doi:10.1046/j.1432-0436.1994.5720143.x

Cited by 44 publications

(71 citation statements)

References 26 publications

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“…The antibody against the first phosphatase domain of human RPTPa was from BD Transduction Laboratories (San Diego, CA, USA). Anti-prostalic acid phosphatase (PAcP) antibody has been described previously (Lin et al 1992). Anti-extracellular-signalregulated kinase (ERK)1/2 (K-23), anti-CgA (C-20), anti-CgB (C-19), anti-parathyroid hormone-related peptide (PTHrP) (N-19), anti-Neurotensin (NT) (C-19), anti-keratin 8/18 (C51), anti-Bcl-2 (100), anti-AR (C-19) and anti-PSA (C-19) antibodies were all from Santa Cruz Biotechnology (Santa Cruz, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…LNCaP and PC-3 cells were routinely maintained in the regular medium (Phenol Red-positive RPMI 1640 medium supplemented with 5% FBS, 1% glutamine, and 0.5% gentamicin) as described previously (Lin et al 1992, Zelivianski et al 2001. NE-1.3, NE-1.8, and NE-1.9 cells were maintained in an SR medium (Phenol Redfree RPMI 1640 medium supplemented with 5% charcoal/dextran-treated, heat-inactivated FBS, 1% glutamine, and 0.5% gentamicin; Zelivianski et al 2001, Zhang et al 2003.…”

Section: Cell Culture and Cdna Transfectionmentioning

confidence: 99%

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Androgen deprivation induces human prostate epithelial neuroendocrine differentiation of androgen-sensitive LNCaP cells

Yuan¹,

Veeramani²,

Lin³

et al. 2006

Endocr Relat Cancer

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145

137

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Neuroendocrine (NE) cells are the minor cell populations in normal prostate epithelial compartments. During prostate carcinogenesis, the number of NE cells in malignant lesions increases, correlating with its tumorigenicity and hormone-refractory growth. It is thus proposed that cancerous NE cells promote prostate cancer (PCa) cell progression and its androgen-independent proliferation, although the origin of the cancerous NE cells is not clear. To investigate the role of cancerous NE cells in prostate carcinogenesis, we characterized three NE subclone cell lines-NE-1.3, NE-1.8 and NE-1.9, which were transdifferentiated from androgen-sensitive human PCa LNCaP cells by culturing in an androgen-depleted environment, resembling clinical androgenablation therapy. These subclone cells acquire many features of NE cells seen in clinical prostate carcinomas, for example exhibiting a neuronal morphology and expressing multiple NE markers, including neuron-specific enolase, chromogranin B, neurotensin, parathyroid hormone-related peptide, and to a lesser degree for chromogranin A, while lacking androgen receptor (AR) or prostate specific antigen (PSA) expression. These cells represent terminally differentiated stable cells because after 3 months of re-culturing in a medium containing androgenic activity, they still retained the NE phenotype and expressed NE markers. Despite these NE cells having a slow growth rate, they readily developed xenograft tumors. Furthermore, media conditioned by these NE cells exhibited a stimulatory effect on proliferation and PSA secretion by LNCaP cells in androgendeprived conditions. Additionally, we found that receptor protein tyrosine phosphatase a plays a role in upregulating multiple NE markers and acquiring the NE phenotype. These NE cells thus represent cancerous NE cells and could serve as a useful cell model system for investigating the role of cancerous NE cells in hormone-refractory proliferation of PCa cells.

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Section: Methodsmentioning

confidence: 99%

Section: Cell Culture and Cdna Transfectionmentioning

confidence: 99%

Androgen deprivation induces human prostate epithelial neuroendocrine differentiation of androgen-sensitive LNCaP cells

Yuan¹,

Veeramani²,

Lin³

et al. 2006

Endocr Relat Cancer

Self Cite

145

137

View full text Add to dashboard Cite

show abstract

“…Furthermore, point mutations are found in PAcP mRNA isolated from cancerous tissues, although the biological significance remains to be clarified (Vihko et al, 1988). Several lines of evidence support the notion that cPAcP functions as a neutral PTPase in prostate epithelial cells Clinton, 1986, 1988;Vihko et al, 1993;Landry et al, 1996) and downregulates cell growth by dephosphorylating phosphotyrosine (p-Tyr) of ErbB-2 protein (Lin et al, 1994;Lin and Meng, 1996;Meng and Lin, 1998;Zhang et al, 2001). It is further proposed that the interaction of cPAcP and ErbB-2 is involved in regulating androgen stimulation of human prostate cancer cell proliferation Meng et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Tyrosine-317 of p52Shc mediates androgen-stimulated proliferation signals in human prostate cancer cells

2004

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“…Several lines of evidence indicate that cellular PAcP functions as a neutral PTP (40 -42) and is involved in the regulation of proliferation and androgen responsiveness of human prostate cancer cells (36,37,39). The intrinsic PTP activity of the enzyme is apparently critical for its functional role in the cancer cells (43,44,55).…”

Section: Discussionmentioning

confidence: 99%

“…In human prostate cancer cells, the cellular level of the enzyme is inversely corre-lated with the cell growth rate. For instance, LNCaP cells that express cellular PAcP have a slow growth rate, compared with PC-3 and DU-145 cells that lack the endogenous PAcP expression (36,37). Introduction of cellular PAcP into prostate cancer cells results in a decreased growth rate (36 -38).…”

mentioning

confidence: 99%

Characterization of a Prostate-specific Tyrosine Phosphatase by Mutagenesis and Expression in Human Prostate Cancer Cells

Zhang¹,

Lee²,

Zelivianski³

et al. 2001

Journal of Biological Chemistry

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The cellular form of human prostatic acid phosphatase (PAcP) is a neutral protein-tyrosine phosphatase (PTP) and may play a key role in regulating the growth and androgen responsiveness of prostate cancer cells. The functional role of the enzyme is at least due in part to its dephosphorylation of c-ErbB-2, an in vivo substrate of the enzyme. In this study, we investigated the molecular mechanism of phosphotyrosine dephosphorylation by cellular PAcP. We mutated several amino acid residues including one cysteine residue that was proposed to be involved in the PTP activity of the enzyme by serving as the phosphate acceptor.

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The cellular level of prostatic acid phosphatase and the growth of human prostate carcinoma cells

Cited by 44 publications

References 26 publications

Androgen deprivation induces human prostate epithelial neuroendocrine differentiation of androgen-sensitive LNCaP cells

Androgen deprivation induces human prostate epithelial neuroendocrine differentiation of androgen-sensitive LNCaP cells

Tyrosine-317 of p52Shc mediates androgen-stimulated proliferation signals in human prostate cancer cells

Characterization of a Prostate-specific Tyrosine Phosphatase by Mutagenesis and Expression in Human Prostate Cancer Cells

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