The cell shedding rate of the corneal epithelium — a comparison of collection methods

Ren, Hongwei; Wilson, Graeme

doi:10.3109/02713689609017655

Cited by 23 publications

(21 citation statements)

References 13 publications

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“…The flattened superficial epithelial cells are shed or sloughed from the corneal surface by mechanical forces associated with blinking. [3][4][5][6] The corneal cell progression requires 7 to 14 days. 6 Notably, the wing cells interdigitate and are bound tightly by numerous desmosomes and gap junctions.…”

Section: Corneal Glu Transporters Ggt and Gsmentioning

confidence: 99%

Glutamate, Excitatory Amino Acid Transporters, Xc⁻Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

Langford¹,

Redmond²,

Chanis³

et al. 2010

Current Eye Research

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The localization of glutamate, multiple EAATs, Xc(-) antiporter proteins, and GGT to columnar and superficial epithelial cell layers suggests uptake of glutamate and cystine from the stroma and tear and supports their importance in regulation of glutamate/cystine and glutathione (GSH; a tripeptide of glutamate, cystine, and glycine) in the human cornea epithelium. In addition, the low glutamate levels in outer wing and flat superficial epithelial cells positive for Xc(-) antiporter and GS are consistent with exchange of glutamate by Xc(-) antiporter for extracellular cystine utilized in GSH synthesis and support coupling of ammonia detoxification with glutamate degradation by GS.

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Section: Corneal Glu Transporters Ggt and Gsmentioning

confidence: 99%

Glutamate, Excitatory Amino Acid Transporters, Xc⁻Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

Langford¹,

Redmond²,

Chanis³

et al. 2010

Current Eye Research

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“…The cornea was then excised and a whole mount cornea was prepared as described by Ren & Wilson (1996b). Corneal surface cells were observed under epi-illumination.…”

Section: Counting Of Shed Epithelial Cellsmentioning

confidence: 99%

“…Corneal surface cells were observed under epi-illumination. The number of non-viable, ethidium positive cells on the corneal surface was counted within a 0.77 mm2 frame on flatmount preparations as described previously (Ren & Wilson 1996b). Non-viable cells on a total of twelve corneas were counted (six paired corneas).…”

Section: Counting Of Shed Epithelial Cellsmentioning

confidence: 99%

The effect of a shear force on the cell shedding rate of the corneal epithelium

Ren¹,

Wilson

1997

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: During blinking the lids apply a shear force to the corneal epithelium. The aim of this study was to determine if a shear force applied to the epithelial surface increases the rate at which cells shed. Methods: The shedding rate was studied in perfused whole rabbit eyes, and the effect of a shear force examined by exposing the corneas to a stirred solution. Control corneas were exposed to a static solution. The shedding rate and size of shed cells were measured, and the number of terminally differentiated cells on the corneal surface determined after 6 h of perfusion using ethidium bromide. Results: Compared with controls, the shear force increased the cell shedding rate from the corneal surface significantly (p < 0.01, paired t-test). The increase was due to small cells with a longest dimension less than 25 pm. The number of terminally differentiated cells on the epithelial surface did not increase. Conclusiom Because of the decrease in size, and the change in appearance of shedding cells, it is proposed that the increase in cell shedding rate was due to an increase in the number of apoptotic cells, and not to an increase in terminally differentiated cells. It is suggested that in the human eye, under adverse conditions, shear forces due to blinking may play a role in creating apoptotic cells.

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“…A szaruhártya hámgyógyulása három egymással össze függő fázisból tevődik össze [11][12][13][14]. Cornealis hámhi ány keletkezésekor a gyógyulási folyamat első 4-6 órájá ban játszódik le a latens fázis, amelynek során a basalis epithelsejtek migrációja zajlik a sebszélről [15].…”

Section: Megbeszélésunclassified

Crosslinking kezelés hatása a szaruhártya hámosodására

et al. 2017

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Bevezetés és célkitűzés:A crosslinking kezelés szaruhártyahámosodásra gyakorolt hatásának vizsgálata. Betegek és mód-szer: Vizsgálatunkba 39 keratoconusos beteget vontunk be crosslinking kezelés után, és 11 pácienst fotorefraktív keratectomiát követően (kontroll) crosslinking kezelést követően 21, 45 és 69 órával. A festődő szaruhártyahámhi ány területét a fotorefraktív keratectomiát követően 16, 37 és 61 órával meghatároztuk. A fotorefraktív keratectomia kezelések utáni kontrollok időpontja nem egyezett a crosslinking kezelést követő kontrollidőpontokkal, így a fotoref raktív keratectomiás csoportnál a hámhiány feltehető méretét a crosslinking kezelési csoport mérési időpontjaira számítottuk, és χ 2 teszt segítségével hasonlítottuk össze a két csoportban a hámhiány méretét. Eredmények: A cornea crosslinking kezelést követően a szaruhártya hámjának záródása a kezeléstől számított három napon belül, fotorefrak tív keratectomiát követően négy napon belül következett be. 45 és 69 óra elteltével a két csoport között szignifikáns különbség igazolódott a hámhiány méretében (p = 0,005 és p<0,001). Következtetés: A crosslinking kezelést követő en a szaruhártya hámosodása gyorsabb, mint fotorefraktív keratectomiát követően. Vizsgálatunk a crosslinking keze lés hámgyógyulásra kifejtett kedvező hatását igazolta. Orv. Hetil., 2017, 158(10), 376-379. Kulcsszavak: crosslinking, fotorefraktív keratectomia, hámgyógyulás Impact of crosslinking on corneal epithelial healingIntroduction and aim: To analyse the effect of crosslinking (CXL) on corneal epithelial healing. Patients and method: 39 keratoconus patients following CXL and 11 patients following PRK were enrolled in this study. 21, 45 and 69 hours after CXL and 16, 37 and 61 hours following PRK slitlamp photographs were taken using fluorescein staining and the size of the epithelial defect and the time of epithelial closure was determined. As the observation timepoints differed in both groups, the size of the epithelial defect following PRK was calculated for the time points used for analysis of CXL patients. These data were compared using chiquadrat test. Results: Following crosslinking, epithe lial closure occured within 3, following PRK within 4 days. In the area of epithelial erosion 45 and 69 hours after CXL or PRK treatment statistically significant difference (p = 0.005 and p<0.001) was verified. Conclusion: Our re sults show beneficial effect of crosslinking on corneal epithelial healing.

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The cell shedding rate of the corneal epithelium — a comparison of collection methods

Cited by 23 publications

References 13 publications

Glutamate, Excitatory Amino Acid Transporters, Xc⁻Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

Glutamate, Excitatory Amino Acid Transporters, Xc⁻Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

The effect of a shear force on the cell shedding rate of the corneal epithelium

Crosslinking kezelés hatása a szaruhártya hámosodására

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The cell shedding rate of the corneal epithelium — a comparison of collection methods

Cited by 23 publications

References 13 publications

Glutamate, Excitatory Amino Acid Transporters, Xc−Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

Glutamate, Excitatory Amino Acid Transporters, Xc−Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

The effect of a shear force on the cell shedding rate of the corneal epithelium

Crosslinking kezelés hatása a szaruhártya hámosodására

Contact Info

Product

Resources

About

Glutamate, Excitatory Amino Acid Transporters, Xc⁻Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium

Glutamate, Excitatory Amino Acid Transporters, Xc⁻Antiporter, Glutamine Synthetase, and γ-Glutamyltranspeptidase in Human Corneal Epithelium