The cell in the field of gravity and the centrifugal field

Moroz, Pavel E.

doi:10.1016/s0022-5193(84)80030-2

Cited by 16 publications

(1 citation statement)

References 37 publications

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“…Optimization is required especially when working with fragile and sensitive cells. On the other hand, smaller/lighter cells require higher centrifugal forces for their efficient capture (Moroz, 1984). We performed a literature search and found only one example where the optimization of centrifugal force was discussed (10x Genomics, 2017).…”

Section: Discussionmentioning

confidence: 99%

Preparation of single-cell suspension from mouse breast cancer focusing on preservation of original cell state information and cell type composition

Pavel

Sandra

Jaroslav

et al. 2019

Preprint

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Single-cell analysis of gene expression has become a very popular method during the last decade.Unfortunately, appropriate standardization and workflow optimization remain elusive. The first step of the single cell analysis requires that the solid tissue be disassociated into a suspension of individual cells. However, during this step several technical bias can arise which can later result in the misinterpretation of the data. The goal of this study was to identify and quantify the effect of these technical factors on the quality of the single-cell suspension and the subsequent interpretation of the produced expression data. We tested the effects of various enzymes used for dissociation, several centrifugation forces, dissociation temperatures and the addition of Actinomycin D, a gene expression inhibitor. RT-qPCR was used to assess the effect from each parameter alteration, while a single-cell RNA sequencing experiment was used to confirm the optimized factors. Our concluding results provide a complete protocol for the tissue dissociation of mouse mammary tumour from 4T1 cells that preserves the original cell state and is suitable for any single-cell RNA sequencing analysis. Furthermore, our workflow may serve as a guide for the optimization of the dissociation procedure of any other tissue of interest, which would ultimately improve the reproducibility of the reported data.

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Section: Discussionmentioning

confidence: 99%

Preparation of single-cell suspension from mouse breast cancer focusing on preservation of original cell state information and cell type composition

Pavel

Sandra

Jaroslav

et al. 2019

Preprint

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Gravitational Zoology: How Animals Use and Cope with Gravity

Anken¹,

Rahmann²

2002

Astrobiology

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Strategies for “minimal growth maintenance” of cell cultures: A perspective on management for extended duration experimentation in the microgravity environment of a space station

Krikorian

1996

Bot. Rev

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How cells manage without gravity and how they change in the absence of gravity are basic questions that only prolonged life on a Space station will enable us to answer. We know from investigations carried out on various kinds of Space vehicles and stations that profound physiological effects can and often to occur. We need to know more of the basic biochemistry and biophysics both of cells and of whole organisms in conditions of reduced gravity. The unique environment of Space affords plant scientists an unusual opportunity to carry out experiments in microgravity, but some major challenges must be faced before this can be done with confidence. Various laboratory activities that are routine on Earth take on special significance and offer problems that need imaginative resolution before even a relatively simple experiment can be reliably executed on a Space station. For example, scientists might wish to investigate whether adaptive or other changes that have occurred in the environment of Space are retained after return to Earth-normal conditions. Investigators seeking to carry out experiments in the low-gravity environment of Space using cultured cells will need to solve the problem of keeping cultures quiescent for protracted periods before an experiment is initiated, after periodic sampling is carried out, and after the experiment is completed. This review gives an evaluation of a range of strategies that can enable one to manipulate cell physiology and curtail growth dramatically toward this end. These strategies include cryopreservation, chilling, reduced oxygen, gel entrapment strategies, osmotic adjustment, nutrient starvation, pH manipulation, and the use of mitotic inhibitors and growth-retarding chemicals. Cells not only need to be rendered quiescent for protracted periods but they also must be recoverable and further grown if it is so desired. Elaboration of satisfactory procedures for management of cells and tissues at "near zero or minimal growth" will have great value and practical consequences for experimentation on Earth as well as in Space. All of the parameters and conditions and procedural details needed to meet all the specific objectives will be the basis of the design and fabrication of cell culture units for use in the Space environment. It is expected that this will be an evolutionary process.

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The cell in the field of gravity and the centrifugal field

Cited by 16 publications

References 37 publications

Preparation of single-cell suspension from mouse breast cancer focusing on preservation of original cell state information and cell type composition

Preparation of single-cell suspension from mouse breast cancer focusing on preservation of original cell state information and cell type composition

Gravitational Zoology: How Animals Use and Cope with Gravity

Strategies for “minimal growth maintenance” of cell cultures: A perspective on management for extended duration experimentation in the microgravity environment of a space station

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