The CD69 receptor: a multipurpose cell-surface trigger for hematopoietic cells

Testi, Roberto; D’Ambrosio, Daniele; Maria, Ruggero De; Santoni, Angela

doi:10.1016/0167-5699(94)90193-7

Cited by 423 publications

(327 citation statements)

References 35 publications

Supporting

Mentioning

314

Contrasting

Unclassified

Order By: Relevance

“…CD69 can be up-regulated as early as 2 h after Ag encounter (our unpublished findings; Ref. 37). Ag-reactive cells demonstrated enhanced CD69 expression before undergoing cell division, as evident from the high level of expression by undivided cells on day 2 after injection of peptide-pulsed DCs (Fig.…”

Section: Phenotype and Recirculation Pattern Of T Cells Activated In mentioning

confidence: 67%

Induction of Rapid T Cell Activation, Division, and Recirculation by Intratracheal Injection of Dendritic Cells in a TCR Transgenic Model

Lambrecht

Pauwels

Groth

2000

The Journal of Immunology

167

173

View full text Add to dashboard Cite

Dendritic cells (DCs) are thought to be responsible for sensitization to inhaled Ag and induction of adaptive immunity in the lung. The characteristics of T cell activation in the lung were studied after transfer of Ag-pulsed bone marrow-derived DCs into the airways of naive mice. Cell division of Ag-specific T cells in vivo was followed in a carboxyfluorescein diacetate succinimidyl ester-labeled cohort of naive moth cytochrome c-reactive TCR transgenic T cells. Our adoptive transfer system was such that transferred DCs were the only cells expressing the MHC molecule required for presentation of cytochrome c to transgenic T cells. Ag-specific T cell activation and proliferation occurred rapidly in the draining lymph nodes of the lung, but not in nondraining lymph nodes or spleen. No bystander activation of non-Ag-specific T cells was induced. Division of Ag-specific T cells was accompanied by transient expression of CD69, while up-regulation of CD44 increased with each cell division. Divided cells had recirculated to nondraining lymph nodes and spleen by day 4 of the response. In vitro restimulation with specific Ag revealed that T cells were primed to proliferate more strongly and to produce higher amounts of cytokines per cell. These data are consistent with the notion that DCs in the lung are extremely efficient in selecting Ag-reactive T cells from a diverse repertoire. The response is initially localized in the mediastinal lymph nodes, but subsequently spreads systemically. This system should allow us to study the early events leading to sensitization to inhaled Ag.

show abstract

Section: Phenotype and Recirculation Pattern Of T Cells Activated In mentioning

confidence: 67%

Induction of Rapid T Cell Activation, Division, and Recirculation by Intratracheal Injection of Dendritic Cells in a TCR Transgenic Model

Lambrecht

Pauwels

Groth

2000

The Journal of Immunology

167

173

View full text Add to dashboard Cite

show abstract

“…In conclusion, sequence analysis and chromosomal assignment identify AICL as a new protein encoded by the human NK gene complex whose expression kinetics resemble CD69 (Testi et al 1994). These findings imply that the NK gene complex, next to NK cell receptors, encodes activation-induced molecules expressed by different types of hematopoietic cells.…”

Section: Aicl Is Encoded By a Single Gene Within The Human Nk Gene Comentioning

confidence: 76%

“…Localization of the CD69 gene in the NK gene complex has been surprising, since function of this molecule is not restricted to NK cells. Cross-linking of the early activation antigen CD69 triggers cell type-specific activation steps in T cells, B cells, NK cells, granulocytes, monocytes, and platelets (Testi et al 1994). The variety of the induced processes indicates that CD69 functions as a central molecule in cellular activation cascades.…”

mentioning

confidence: 99%

AICL: a new activation-induced antigen encoded by the human NK gene complex

et al. 1997

View full text Add to dashboard Cite

show abstract

“…However, T and/or B cell deficiency had no significant effect on the acquisition of CD43 and CD11b by BM or spleen NK cells (data not shown). Further, CD69 is a marker for recently activated lymphocytes [34], and CD62L down-regulation upon activation allows the extravasation of immune effector cells to sites of inflammation or injury [35]. The percentages of CD69 + and CD62L low NK cells showed considerable variation between individual mice.…”

Section: Markers Of Activation or Maturation On Nk Cells In Lymphopenmentioning

confidence: 99%

T and B lymphocytes exert distinct effects on the homeostasis of NK cells

2006

View full text Add to dashboard Cite

There is growing evidence that lymphocytes impact the development and/or function of other lymphocyte populations. Based on such observations we have tested whether the NK cell compartment was phenotypically and functionally altered in the absence of B and/or T cells. Here we show that T cell deficiency significantly accelerates BM NK cell production and the subsequent seeding of splenic and liver NK cell compartments. In contrast, B cell deficiency reduces splenic NK cell survival. In the absence of T and B cells, the size of the NK cell compartments is determined by the combination of these positive and negative effects. Even though NK cell homeostasis is significantly altered, NK cells from T and/or B cell-deficient mice show a normal capacity to kill a susceptible target cell line and to produce IFN. Nevertheless, we noted that the usage of MHC class Ispecific Ly49 family receptors was significantly altered in the absence of T and/or B cells. In general, B cell deficiency expanded Ly49 receptor usage, while T cell deficiency exerted both positive and negative effects. These findings show that B and T cells significantly and differentially influence the homeostasis and the phenotype of NK cells.

show abstract

The CD69 receptor: a multipurpose cell-surface trigger for hematopoietic cells

Cited by 423 publications

References 35 publications

Induction of Rapid T Cell Activation, Division, and Recirculation by Intratracheal Injection of Dendritic Cells in a TCR Transgenic Model

Induction of Rapid T Cell Activation, Division, and Recirculation by Intratracheal Injection of Dendritic Cells in a TCR Transgenic Model

AICL: a new activation-induced antigen encoded by the human NK gene complex

T and B lymphocytes exert distinct effects on the homeostasis of NK cells

Contact Info

Product

Resources

About