“…The extracts were subjected to reverse phase HPLC and radiolabeled glycopeptide fraction isolation, pooled and automatically sequenced to confirm the glycopeptide identity, corresponding to one single N‐terminal sequence (Fairhall & Robinson, ). The antisera obtained was tested for binding to radioiodinated glycopeptide, and cross‐reactivity (AVP, k.1.7 rabbit polyclonal, Fairhall & Robinson, , kindly provided by Dr. van Leeuwen, RRID: AB_2732873): anti‐OXT monoclonal antibodies were produced by mouse spleen cell hybridoma and tested for specificity via radiobinding testing, and immunoabsorption with synthetic AVP, pressinamide, OT, (2‐Phe)OT, (3‐Phe)OT, (8‐Ile)OT, (4‐Asn)OT, (7‐Gly)OT, (8‐Val)OT, AVT, and tocinamide (A1–28, mouse monoclonal antibody, generous gift of A. J. Silverman, used in Hou‐Yu, Lamme, Zimmerman, & Silverman, , RRID: AB_2732874). The AVP C‐terminal end is extended by a 39‐peptides glycopeptide, which is not present in the OXT precursor providing a specific target for antibodies (Fairhall & Robinson, ; Gordon‐Weeks, Jones, & Robinson, ; Richards, Morris, & Raisman, ).…”