The canine mast cell activation via CRP

Fujimoto, Takahiro; Sato, Yoshitaka; Sasaki, Nobuo; Teshima, Reiko; Hanaoka, Kazuo; Kitani, Seiichi

doi:10.1016/s0006-291x(02)03009-7

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…It was reported that CRP induced a transient calcium mobilization dose dependently in canine mast cells [27]. However, it has not been known yet whether CRP increased intracellular calcium in human mesangial cells and also whether CRP-induced intracellular calcium was involved in the CRP-induced NF-ĸB activation.…”

Section: Discussionmentioning

confidence: 99%

C-Reactive Protein Induces NF-κB Activation through Intracellular Calcium and ROS in Human Mesangial Cells

Chang

Kim²,

Kim³

et al. 2005

Nephron Exp Nephrol

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Background: C-reactive protein (CRP) is known to have a direct proinflammatory effect in endothelial cells. However, little is known about the effect of CRP in intrinsic renal cells. We investigated the effects of CRP on the nuclear factor-ĸB (NF-ĸB) activation and monocyte chemoattractant protein-1 (MCP-1) gene expression in human mesangial cells and also examined whether intracellular calcium and reactive oxygen species (ROS) were involved in the CRP- induced NF-ĸB activation. Methods: NF-ĸB binding activity and MCP-1 mRNA expression were measured by electrophoretic mobility shift assay and Northern blot analysis, respectively.Intracellular calcium was monitored by confocal microscopy using calcium sensitive dye, Fluo-3 and intracellular ROS production was determined, using 2′,7′-dichlorofluorescin diacetate. Results: CRP increased NF-ĸB binding activity in a dose-dependent manner (12.5–100 µg/ml), which was induced within 1 h after incubation and peaked around 3 h. CRP also increased the MCP-1 mRNA expression via activation of NF-ĸB. Both intracellular calcium and ROS was induced by CRP. Calcium chelator, BAPTA-AM and anti-oxidants such as N-acetylcysteine and tiron suppressed CRP-induced NF-ĸB activation. Conclusion: CRP exerted a proinflammatory effect in human mesangial cells by inducing MCP-1 gene expression via NF-ĸB activation, which was mediated, at least in part, through intracellular calcium and ROS.

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Section: Discussionmentioning

confidence: 99%

C-Reactive Protein Induces NF-κB Activation through Intracellular Calcium and ROS in Human Mesangial Cells

Chang

Kim²,

Kim³

et al. 2005

Nephron Exp Nephrol

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“…There are very few reports of a useful marker that reflects the status of the local immune response. Fujimoto et al [36] reported that CRP-mediated recruitment of MCs might play an important role in inflammatory processes in a canine model. The negative correlation between them is possibly due to CRP being consumed in the recruitment of MCs to tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of peritumoral mast cells in esophageal squamous cell carcinoma with neoadjuvant chemoradiotherapy

et al. 2012

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Background Neoadjuvant chemoradiotherapy (CRT) followed by surgery is the standard approach for locally advanced esophageal cancer. Recently, the microenvironment (including the host immune response) after conventional chemo-and/or radiotherapy has been highlighted. The aim of this preliminary study was to evaluate peritumoral mast cells (MCs) in esophageal squamous cell carcinoma (ESCC) after neoadjuvant CRT. Methods We obtained a total of twenty specimens from patients with ESCC who underwent neoadjuvant CRT (30-40 Gy; 5-fluorouracil plus cisplatin) followed by surgery. We evaluated the expression of tryptase by MCs, Foxp3 by regulatory T cells, CD8 by cytotoxic T cells, and microvessel density (MVD) using immunohistochemistry. We investigated the correlation between the sizes of these marker-positive cell populations surrounding residual tumor nests, MVD and CRP, and clinical outcome. Results Patients with a low number of peritumoral MCs more frequently had lymphatic invasion (P = 0.0191). There was no significant correlation among the sizes of the marker-positive cell populations. We observed a significant negative correlation between the number of MCs and preoperative CRP levels (P = 0.009). Low numbers of peritumoral MCs, high MVD, and a preoperative C-reactive protein of [0.5 mg/dL were significantly associated with poor overall survival (MCs: P = 0.0239; MVD: P = 0.0317; CRP: P = 0.0395). Conclusion Our results suggest that peritumoral MCs may be associated with prognosis in patients with ESCC after neoadjuvant CRT.

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“…Serum amyloid A (SAA), an acute-phase protein, has been shown to induce mast cell migraton through a pertussis toxin-sensitive signal transduction pathway (226). Canine mast cells can be activated by C Reative Protein (CRP) in a G-protein mediated activation (227). The human cathelicidin-derived antibacterial peptide, LL-37 has been shown to induce mast cell chemotaxis through specific receptors coupled to the G protein-phospholipase C (PLC) signalling pathway (228).…”

Section: Mast Cell Recruitmentmentioning

confidence: 99%

Origin maturation and recruitment of mast cell precursors

Jamur¹

2011

Front Biosci

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Introduction 3. Origin of mast cell committed precursors 3.1. Mouse bone marrow derived mast cell committed precursor 3.2. Human bone marrow derived mast cell committed precursor 4. Mast cell maturation 4.1. Maturation of bone marrow derived mast cells 4.2. Maturation of mast cells at peripheral sites 4.3. Major factors affecting mast cell proliferation and maturation 5. Mast cell recruitment 6. Conclusions 7. References

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The canine mast cell activation via CRP

Cited by 11 publications

References 23 publications

C-Reactive Protein Induces NF-κB Activation through Intracellular Calcium and ROS in Human Mesangial Cells

C-Reactive Protein Induces NF-κB Activation through Intracellular Calcium and ROS in Human Mesangial Cells

Clinical significance of peritumoral mast cells in esophageal squamous cell carcinoma with neoadjuvant chemoradiotherapy

Origin maturation and recruitment of mast cell precursors

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