The calcium‐dependent kinase OsCPK24 functions in cold stress responses in rice

Liu, Yu; Xu, Chunjue; Zhu, Yanfen; Zhang, Lina; Chen, Taiyu; Zhou, Fei; Chen, Hao

doi:10.1111/jipb.12614

Cited by 80 publications

(55 citation statements)

References 56 publications

(68 reference statements)

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“…Cold tolerance is a complex trait in plants. In rice, many genes and QTLs associated with cold stress have been identified, providing valuable genetic resources for enhancing cold tolerance through molecular breeding (Andaya and Mackill, ; Fujino et al ., ; Liu et al ., ,b; Lu et al ., ; Ma et al ., ; Mao et al ., ; Saito et al ., ; Zhang et al ., ; Zhao et al ., , ). The QTL qLTG3‐1 controls low‐temperature germinability, and a 71‐bp deletion in the coding region of qLTG3‐1 causes a decrease in low‐temperature germinability in rice variety Hayamasari (Fujino et al ., ).…”

Section: Discussionmentioning

confidence: 99%

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The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

Zhao

Wang

Qin

et al. 2019

Plant Biotechnology Journal

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Summary Nonspecific lipid transfer proteins (nsLTPs) play critical roles in plant development and response to abiotic stresses. Here, we found that a rice lipid transfer protein, OsLTPL159, was associated with cold tolerance at the early seedling stage. Overexpression of an OsLTPL159IL112 allele from the cold‐tolerant introgression line IL112 in either the japonica variety Zhonghua17 (ZH17) or the indica variety Teqing background dramatically enhanced cold tolerance. In addition, down‐regulation of the expression of OsLTPL159 in the japonica variety ZH17 by RNA interference (RNAi) significantly decreased cold tolerance. Further transcriptomic, physiological and histological analysis showed that the OsLTPL159IL112 allele likely enhanced the cold tolerance of rice at the early seedling stage by decreasing the toxic effect of reactive oxygen species, enhancing cellulose deposition in the cell wall and promoting osmolyte accumulation, thereby maintaining the integrity of the chloroplasts. Notably, overexpression of another allele, OsLTPL159GC2, from the recipient parent Guichao 2 (GC2), an indica variety, did not improve cold tolerance, indicating that the variations in the OsLTPL159 coding region of GC2 might disrupt its function for cold tolerance. Further sequence comparison found that all 22 japonica varieties surveyed had an OsLTPL159 haplotype identical to IL112 and were more cold‐tolerant than the surveyed indica varieties, implying that the variations in OsLTPL159 might be associated with differential cold tolerance of japonica and indica rice. Therefore, our findings suggest that the OsLTPL159 allele of japonica rice could be used to improve cold tolerance of indica rice through a molecular breeding strategy.

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Section: Discussionmentioning

confidence: 99%

“…Free proline concentration was measured as described previously (Liu et al ., ). Approximately 200 mg early seedling tissue was homogenized in 2 mL sulphosalicylic acid (3%), followed by centrifugation at 13 000 g for 15 min at 4 °C.…”

Section: Methodsmentioning

confidence: 97%

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

Zhao

Wang

Qin

et al. 2019

Plant Biotechnology Journal

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“…Proline is an essential proteinogenic amino acid and plays important roles in plant abiotic-stress tolerance (Nanjo et al 1999 ; Székely et al 2008 ; Zhang et al 2017 ; Liu et al 2018 ). To date, much is known about proline synthesis and metabolism in higher plants.…”

Section: Resultsmentioning

confidence: 99%

OsProDH Negatively Regulates Thermotolerance in Rice by Modulating Proline Metabolism and Reactive Oxygen Species Scavenging

Guo

Zhang

et al. 2020

Rice

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Background Global warming threatens rice growth and reduces yields. Proline plays important roles in plant abiotic stress tolerance. Previous research demonstrated that engineering proline metabolism-related genes can enhance tolerance to freezing and salinity in Arabidopsis . OsProDH encodes a putative proline dehydrogenase and is a single copy gene in rice. However, whether OsProDH plays roles in abiotic stress in rice remains unknown. Findings Quantitative RT-PCR analysis revealed that OsProDH transcript contents were relatively higher in leaf blade and root tissues and the high temperature treatment repressed expression of OsProDH . The predicted OsProDH protein localized in mitochondria. Using the Oryza sativa ssp. japonica cultivar KY131, we generated OsProDH overexpression (OE) lines and knockout mutant lines using the CRISPR/Cas9 (CRI) system. Overexpression of OsProDH decreased proline content, while mutation of OsProDH increased proline content compared with that of KY131. The CRI and OE lines were respectively more resistant and sensitive to heat stress than KY131. Heat stress induced proline accumulation and mutation of OsProDH led to proline overproduction which reduced H 2 O 2 accumulation in the seedlings. Conclusions OsProDH negatively regulates thermotolerance in rice. Our study provides a strategy to improve heat tolerance in rice via manipulating proline metabolism.

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“…OsGSK1 ( GLYCOGEN SYNTHASE KINASE3‐LIKE GENE 1), a GSK3/SHAGGY ‐like protein kinase gene and an ortholog of Arabidopsis BRASSINOSTEROID INSENSITIVE 2 ( BIN2 ), negatively regulates chilling tolerance (Koh et al 2007). Whereas, OsCIPK03 (a calcineurin B‐like protein‐interacting kinase encoding gene), OsCPK24 (CALCIUM‐DEPENDENT PROTEIN KINASE 24) improves rice tolerance to chilling stress (Xiang et al 2007; Liu et al 2018c). Additionally, CDPK13 (CALCIUM‐DEPENDENT PROTEIN KINASE 13) and CRTintP1 (CALRETICULIN‐INTERACTING PROTEIN 1) may be important signaling components for rice response to cold stress (Komatsu et al 2007).…”

Section: Chilling Signal Transduction In Ricementioning

confidence: 99%

Coordination of light, circadian clock with temperature: The potential mechanisms regulating chilling tolerance in rice

Zhou

Fan

et al. 2019

JIPB

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Rice (Oryza sativa L.) is a major staple food crop for over half of the world's population. As a crop species originated from the subtropics, rice production is hampered by chilling stress. The genetic mechanisms of rice responses to chilling stress have attracted much attention, focusing on chilling-related gene mining and functional analyses. Plants have evolved sophisticated regulatory systems to respond to chilling stress in coordination with light signaling pathway and internal circadian clock. However, in rice, information about light-signaling pathways and circadian clock regulation and their roles in chilling tolerance remains elusive. Further investigation into the regulatory network of chilling tolerance in rice is needed, as knowledge of the interaction between temperature, light, and circadian clock dynamics is limited. Here, based on phenotypic analysis of transgenic and mutant rice lines, we delineate the relevant genes with important regulatory roles in chilling tolerance. In addition, we discuss the potential coordination mechanism among temperature, light, and circadian clock in regulating chilling response and tolerance of rice, and provide perspectives for the ongoing chilling signaling network research in rice.

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The calcium‐dependent kinase OsCPK24 functions in cold stress responses in rice

Cited by 80 publications

References 56 publications

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

OsProDH Negatively Regulates Thermotolerance in Rice by Modulating Proline Metabolism and Reactive Oxygen Species Scavenging

Coordination of light, circadian clock with temperature: The potential mechanisms regulating chilling tolerance in rice

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